Enrichment of deleterious variants of mitochondrial DNA polymerase gene (POLG1) in bipolar disorder

Takaoki Kasahara, Mizuho Ishiwata, Chihiro Kakiuchi, Satoshi Fuke, Nakao Iwata, Norio Ozaki, Hiroshi Kunugi, Yoshio Minabe, Kazuhiko Nakamura, Yasuhide Iwata, Kumiko Fujii, Shigenobu Kanba, Hiroshi Ujike, Ichiro Kusumi, Muneko Kataoka, Nana Matoba, Atsushi Takata, Kazuya Iwamoto, Takeo Yoshikawa, Tadafumi Kato

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Aim: Rare missense variants, which likely account for a substantial portion of the genetic ‘dark matter’ for a common complex disease, are challenging because the impacts of variants on disease development are difficult to substantiate. This study aimed to examine the impacts of amino acid substitution variants in the POLG1 found in bipolar disorder, as an example and proof of concept, in three different modalities of assessment: in silico predictions, in vitro biochemical assays, and clinical evaluation. We then tested whether deleterious variants in POLG1 contributed to the genetics of bipolar disorder. Methods: We searched for variants in the POLG1 gene in 796 Japanese patients with bipolar disorder and 767 controls and comprehensively investigated all 23 identified variants in the three modalities of assessment. POLG1 encodes mitochondrial DNA polymerase and is one of the causative genes for a Mendelian-inheritance mitochondrial disease, which is occasionally accompanied by mood disorders. The healthy control data from the Tohoku Medical Megabank Organization were also employed. Results: Although the frequency of carriers of deleterious variants varied from one method to another, every assessment achieved the same conclusion that deleterious POLG1 variants were significantly enriched in the variants identified in patients with bipolar disorder compared to those in controls. Conclusion: Together with mitochondrial dysfunction in bipolar disorder, the present results suggested deleterious POLG1 variants as a credible risk for the multifactorial disease.

Original languageEnglish
Pages (from-to)518-529
Number of pages12
JournalPsychiatry and clinical neurosciences
Volume71
Issue number8
DOIs
Publication statusPublished - 08-2017

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DNA-Directed DNA Polymerase
Mitochondrial DNA
Bipolar Disorder
Genes
Mitochondrial Diseases
Inborn Genetic Diseases
Amino Acid Substitution
Mood Disorders
Computer Simulation
Organizations

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)
  • Neurology
  • Clinical Neurology
  • Psychiatry and Mental health

Cite this

Kasahara, Takaoki ; Ishiwata, Mizuho ; Kakiuchi, Chihiro ; Fuke, Satoshi ; Iwata, Nakao ; Ozaki, Norio ; Kunugi, Hiroshi ; Minabe, Yoshio ; Nakamura, Kazuhiko ; Iwata, Yasuhide ; Fujii, Kumiko ; Kanba, Shigenobu ; Ujike, Hiroshi ; Kusumi, Ichiro ; Kataoka, Muneko ; Matoba, Nana ; Takata, Atsushi ; Iwamoto, Kazuya ; Yoshikawa, Takeo ; Kato, Tadafumi. / Enrichment of deleterious variants of mitochondrial DNA polymerase gene (POLG1) in bipolar disorder. In: Psychiatry and clinical neurosciences. 2017 ; Vol. 71, No. 8. pp. 518-529.
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abstract = "Aim: Rare missense variants, which likely account for a substantial portion of the genetic ‘dark matter’ for a common complex disease, are challenging because the impacts of variants on disease development are difficult to substantiate. This study aimed to examine the impacts of amino acid substitution variants in the POLG1 found in bipolar disorder, as an example and proof of concept, in three different modalities of assessment: in silico predictions, in vitro biochemical assays, and clinical evaluation. We then tested whether deleterious variants in POLG1 contributed to the genetics of bipolar disorder. Methods: We searched for variants in the POLG1 gene in 796 Japanese patients with bipolar disorder and 767 controls and comprehensively investigated all 23 identified variants in the three modalities of assessment. POLG1 encodes mitochondrial DNA polymerase and is one of the causative genes for a Mendelian-inheritance mitochondrial disease, which is occasionally accompanied by mood disorders. The healthy control data from the Tohoku Medical Megabank Organization were also employed. Results: Although the frequency of carriers of deleterious variants varied from one method to another, every assessment achieved the same conclusion that deleterious POLG1 variants were significantly enriched in the variants identified in patients with bipolar disorder compared to those in controls. Conclusion: Together with mitochondrial dysfunction in bipolar disorder, the present results suggested deleterious POLG1 variants as a credible risk for the multifactorial disease.",
author = "Takaoki Kasahara and Mizuho Ishiwata and Chihiro Kakiuchi and Satoshi Fuke and Nakao Iwata and Norio Ozaki and Hiroshi Kunugi and Yoshio Minabe and Kazuhiko Nakamura and Yasuhide Iwata and Kumiko Fujii and Shigenobu Kanba and Hiroshi Ujike and Ichiro Kusumi and Muneko Kataoka and Nana Matoba and Atsushi Takata and Kazuya Iwamoto and Takeo Yoshikawa and Tadafumi Kato",
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Kasahara, T, Ishiwata, M, Kakiuchi, C, Fuke, S, Iwata, N, Ozaki, N, Kunugi, H, Minabe, Y, Nakamura, K, Iwata, Y, Fujii, K, Kanba, S, Ujike, H, Kusumi, I, Kataoka, M, Matoba, N, Takata, A, Iwamoto, K, Yoshikawa, T & Kato, T 2017, 'Enrichment of deleterious variants of mitochondrial DNA polymerase gene (POLG1) in bipolar disorder', Psychiatry and clinical neurosciences, vol. 71, no. 8, pp. 518-529. https://doi.org/10.1111/pcn.12496

Enrichment of deleterious variants of mitochondrial DNA polymerase gene (POLG1) in bipolar disorder. / Kasahara, Takaoki; Ishiwata, Mizuho; Kakiuchi, Chihiro; Fuke, Satoshi; Iwata, Nakao; Ozaki, Norio; Kunugi, Hiroshi; Minabe, Yoshio; Nakamura, Kazuhiko; Iwata, Yasuhide; Fujii, Kumiko; Kanba, Shigenobu; Ujike, Hiroshi; Kusumi, Ichiro; Kataoka, Muneko; Matoba, Nana; Takata, Atsushi; Iwamoto, Kazuya; Yoshikawa, Takeo; Kato, Tadafumi.

In: Psychiatry and clinical neurosciences, Vol. 71, No. 8, 08.2017, p. 518-529.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Enrichment of deleterious variants of mitochondrial DNA polymerase gene (POLG1) in bipolar disorder

AU - Kasahara, Takaoki

AU - Ishiwata, Mizuho

AU - Kakiuchi, Chihiro

AU - Fuke, Satoshi

AU - Iwata, Nakao

AU - Ozaki, Norio

AU - Kunugi, Hiroshi

AU - Minabe, Yoshio

AU - Nakamura, Kazuhiko

AU - Iwata, Yasuhide

AU - Fujii, Kumiko

AU - Kanba, Shigenobu

AU - Ujike, Hiroshi

AU - Kusumi, Ichiro

AU - Kataoka, Muneko

AU - Matoba, Nana

AU - Takata, Atsushi

AU - Iwamoto, Kazuya

AU - Yoshikawa, Takeo

AU - Kato, Tadafumi

PY - 2017/8

Y1 - 2017/8

N2 - Aim: Rare missense variants, which likely account for a substantial portion of the genetic ‘dark matter’ for a common complex disease, are challenging because the impacts of variants on disease development are difficult to substantiate. This study aimed to examine the impacts of amino acid substitution variants in the POLG1 found in bipolar disorder, as an example and proof of concept, in three different modalities of assessment: in silico predictions, in vitro biochemical assays, and clinical evaluation. We then tested whether deleterious variants in POLG1 contributed to the genetics of bipolar disorder. Methods: We searched for variants in the POLG1 gene in 796 Japanese patients with bipolar disorder and 767 controls and comprehensively investigated all 23 identified variants in the three modalities of assessment. POLG1 encodes mitochondrial DNA polymerase and is one of the causative genes for a Mendelian-inheritance mitochondrial disease, which is occasionally accompanied by mood disorders. The healthy control data from the Tohoku Medical Megabank Organization were also employed. Results: Although the frequency of carriers of deleterious variants varied from one method to another, every assessment achieved the same conclusion that deleterious POLG1 variants were significantly enriched in the variants identified in patients with bipolar disorder compared to those in controls. Conclusion: Together with mitochondrial dysfunction in bipolar disorder, the present results suggested deleterious POLG1 variants as a credible risk for the multifactorial disease.

AB - Aim: Rare missense variants, which likely account for a substantial portion of the genetic ‘dark matter’ for a common complex disease, are challenging because the impacts of variants on disease development are difficult to substantiate. This study aimed to examine the impacts of amino acid substitution variants in the POLG1 found in bipolar disorder, as an example and proof of concept, in three different modalities of assessment: in silico predictions, in vitro biochemical assays, and clinical evaluation. We then tested whether deleterious variants in POLG1 contributed to the genetics of bipolar disorder. Methods: We searched for variants in the POLG1 gene in 796 Japanese patients with bipolar disorder and 767 controls and comprehensively investigated all 23 identified variants in the three modalities of assessment. POLG1 encodes mitochondrial DNA polymerase and is one of the causative genes for a Mendelian-inheritance mitochondrial disease, which is occasionally accompanied by mood disorders. The healthy control data from the Tohoku Medical Megabank Organization were also employed. Results: Although the frequency of carriers of deleterious variants varied from one method to another, every assessment achieved the same conclusion that deleterious POLG1 variants were significantly enriched in the variants identified in patients with bipolar disorder compared to those in controls. Conclusion: Together with mitochondrial dysfunction in bipolar disorder, the present results suggested deleterious POLG1 variants as a credible risk for the multifactorial disease.

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