Environmental enrichment alters gene expression of steroidogenic enzymes in the rat hippocampus

Eiji Munetsuna, Minoru Hattori, Yuya Sakimoto, Atsuhiko Ishida, Shogo Sakata, Yasushi Hojo, Suguru Kawato, Takeshi Yamazaki

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Neuroactive steroids are synthesized in the central and peripheral nervous systems. The purpose of this study was to analyze the effects of environmental enrichment on neuroactive steroidogenesis in the rat hippocampus. Environmental enrichment rats were housed in a group of nine in a large cage and three groups of pair-housed rats were housed in a standard cage for 8. weeks. The levels of mRNAs for steroidogenic enzymes and proteins in hippocampus were quantified by real-time RT-PCR. Environmental enrichment increased the mRNA expression levels of 5α-reductase-1 and 3α-hydroxysteroid dehydrogenase, which catalyze synthesis of allopregnanolone from progesterone. Hence, environmental enrichment appears to affect allopregnanolone synthesis.

Original languageEnglish
Pages (from-to)28-32
Number of pages5
JournalGeneral and Comparative Endocrinology
Volume171
Issue number1
DOIs
Publication statusPublished - 01-03-2011
Externally publishedYes

Fingerprint

environmental enrichment
hippocampus
Pregnanolone
Hippocampus
Gene Expression
gene expression
rats
Enzymes
3-Hydroxysteroid Dehydrogenases
enzymes
Messenger RNA
cages
Peripheral Nervous System
hydroxysteroid dehydrogenases
peripheral nervous system
steroidogenesis
synthesis
Progesterone
Real-Time Polymerase Chain Reaction
Oxidoreductases

All Science Journal Classification (ASJC) codes

  • Animal Science and Zoology
  • Endocrinology

Cite this

Munetsuna, Eiji ; Hattori, Minoru ; Sakimoto, Yuya ; Ishida, Atsuhiko ; Sakata, Shogo ; Hojo, Yasushi ; Kawato, Suguru ; Yamazaki, Takeshi. / Environmental enrichment alters gene expression of steroidogenic enzymes in the rat hippocampus. In: General and Comparative Endocrinology. 2011 ; Vol. 171, No. 1. pp. 28-32.
@article{4a149794b043490bb6e943b86202a76a,
title = "Environmental enrichment alters gene expression of steroidogenic enzymes in the rat hippocampus",
abstract = "Neuroactive steroids are synthesized in the central and peripheral nervous systems. The purpose of this study was to analyze the effects of environmental enrichment on neuroactive steroidogenesis in the rat hippocampus. Environmental enrichment rats were housed in a group of nine in a large cage and three groups of pair-housed rats were housed in a standard cage for 8. weeks. The levels of mRNAs for steroidogenic enzymes and proteins in hippocampus were quantified by real-time RT-PCR. Environmental enrichment increased the mRNA expression levels of 5α-reductase-1 and 3α-hydroxysteroid dehydrogenase, which catalyze synthesis of allopregnanolone from progesterone. Hence, environmental enrichment appears to affect allopregnanolone synthesis.",
author = "Eiji Munetsuna and Minoru Hattori and Yuya Sakimoto and Atsuhiko Ishida and Shogo Sakata and Yasushi Hojo and Suguru Kawato and Takeshi Yamazaki",
year = "2011",
month = "3",
day = "1",
doi = "10.1016/j.ygcen.2010.12.007",
language = "English",
volume = "171",
pages = "28--32",
journal = "General and Comparative Endocrinology",
issn = "0016-6480",
publisher = "Academic Press Inc.",
number = "1",

}

Environmental enrichment alters gene expression of steroidogenic enzymes in the rat hippocampus. / Munetsuna, Eiji; Hattori, Minoru; Sakimoto, Yuya; Ishida, Atsuhiko; Sakata, Shogo; Hojo, Yasushi; Kawato, Suguru; Yamazaki, Takeshi.

In: General and Comparative Endocrinology, Vol. 171, No. 1, 01.03.2011, p. 28-32.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Environmental enrichment alters gene expression of steroidogenic enzymes in the rat hippocampus

AU - Munetsuna, Eiji

AU - Hattori, Minoru

AU - Sakimoto, Yuya

AU - Ishida, Atsuhiko

AU - Sakata, Shogo

AU - Hojo, Yasushi

AU - Kawato, Suguru

AU - Yamazaki, Takeshi

PY - 2011/3/1

Y1 - 2011/3/1

N2 - Neuroactive steroids are synthesized in the central and peripheral nervous systems. The purpose of this study was to analyze the effects of environmental enrichment on neuroactive steroidogenesis in the rat hippocampus. Environmental enrichment rats were housed in a group of nine in a large cage and three groups of pair-housed rats were housed in a standard cage for 8. weeks. The levels of mRNAs for steroidogenic enzymes and proteins in hippocampus were quantified by real-time RT-PCR. Environmental enrichment increased the mRNA expression levels of 5α-reductase-1 and 3α-hydroxysteroid dehydrogenase, which catalyze synthesis of allopregnanolone from progesterone. Hence, environmental enrichment appears to affect allopregnanolone synthesis.

AB - Neuroactive steroids are synthesized in the central and peripheral nervous systems. The purpose of this study was to analyze the effects of environmental enrichment on neuroactive steroidogenesis in the rat hippocampus. Environmental enrichment rats were housed in a group of nine in a large cage and three groups of pair-housed rats were housed in a standard cage for 8. weeks. The levels of mRNAs for steroidogenic enzymes and proteins in hippocampus were quantified by real-time RT-PCR. Environmental enrichment increased the mRNA expression levels of 5α-reductase-1 and 3α-hydroxysteroid dehydrogenase, which catalyze synthesis of allopregnanolone from progesterone. Hence, environmental enrichment appears to affect allopregnanolone synthesis.

UR - http://www.scopus.com/inward/record.url?scp=79951509852&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=79951509852&partnerID=8YFLogxK

U2 - 10.1016/j.ygcen.2010.12.007

DO - 10.1016/j.ygcen.2010.12.007

M3 - Article

VL - 171

SP - 28

EP - 32

JO - General and Comparative Endocrinology

JF - General and Comparative Endocrinology

SN - 0016-6480

IS - 1

ER -