Epithelial-mesenchymal transition of the eccrine glands is involved in skin fibrosis in morphea

Masayuki Takahashi, Hirohiko Akamatsu, Akiko Yagami, Seiji Hasegawa, Shiroh Ohgo, Masamichi Abe, Yohei Iwata, Masaru Arima, Hiroshi Mizutani, Satoru Nakata, Kayoko Matsunaga

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Morphea is a type of localized scleroderma. It is a skin disease involving the development of fibrosis in the dermis and subcutaneous fat tissue beneath without a visceral lesion, and the cause is still unclear. An involvement of epithelial-mesenchymal transition (EMT) has been reported as a cause of tissue fibrosis, but this was mostly observed in pulmonary and hepatic fibrosis, and the involvement of EMT in a skin disease, morphea, has not been studied. Thus, we analyzed the involvement of EMT in skin fibrosis in morphea patients using pathological techniques. Skin lesions of six morphea patients were analyzed (five female and one male patient). As a control, non-light-exposed skin lesions of 11 healthy females were analyzed. Concretely, tissue samples were prepared from these subjects and subjected to immunostaining of transforming growth factor (TGF)-β1, α-smooth muscle actin (α-SMA) and fibronectin, which have been reported to be associated with fibrosis, and Snail1 and E-cadherin, which are considered to be involved in EMT, and expressions of these were analyzed. In morphea patients, dermal expression of TGF-β1, α-SMA and fibronectin, which are involved in fibrosis, was enhanced, and, at the same time, enhanced expression of Snail1 and reduced expression of E-cadherin, which are involved in EMT, were observed in the dermal eccrine glands. These findings suggested the progression of EMT in the dermal eccrine glands in morphea.

Original languageEnglish
Pages (from-to)720-725
Number of pages6
JournalJournal of Dermatology
Volume40
Issue number9
DOIs
Publication statusPublished - 01-09-2013

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Eccrine Glands
Localized Scleroderma
Epithelial-Mesenchymal Transition
Fibrosis
Skin
Transforming Growth Factors
Cadherins
Fibronectins
Skin Diseases
Pulmonary Fibrosis
Subcutaneous Fat
Subcutaneous Tissue
Dermis
Smooth Muscle
Actins
Liver

All Science Journal Classification (ASJC) codes

  • Dermatology

Cite this

Takahashi, Masayuki ; Akamatsu, Hirohiko ; Yagami, Akiko ; Hasegawa, Seiji ; Ohgo, Shiroh ; Abe, Masamichi ; Iwata, Yohei ; Arima, Masaru ; Mizutani, Hiroshi ; Nakata, Satoru ; Matsunaga, Kayoko. / Epithelial-mesenchymal transition of the eccrine glands is involved in skin fibrosis in morphea. In: Journal of Dermatology. 2013 ; Vol. 40, No. 9. pp. 720-725.
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abstract = "Morphea is a type of localized scleroderma. It is a skin disease involving the development of fibrosis in the dermis and subcutaneous fat tissue beneath without a visceral lesion, and the cause is still unclear. An involvement of epithelial-mesenchymal transition (EMT) has been reported as a cause of tissue fibrosis, but this was mostly observed in pulmonary and hepatic fibrosis, and the involvement of EMT in a skin disease, morphea, has not been studied. Thus, we analyzed the involvement of EMT in skin fibrosis in morphea patients using pathological techniques. Skin lesions of six morphea patients were analyzed (five female and one male patient). As a control, non-light-exposed skin lesions of 11 healthy females were analyzed. Concretely, tissue samples were prepared from these subjects and subjected to immunostaining of transforming growth factor (TGF)-β1, α-smooth muscle actin (α-SMA) and fibronectin, which have been reported to be associated with fibrosis, and Snail1 and E-cadherin, which are considered to be involved in EMT, and expressions of these were analyzed. In morphea patients, dermal expression of TGF-β1, α-SMA and fibronectin, which are involved in fibrosis, was enhanced, and, at the same time, enhanced expression of Snail1 and reduced expression of E-cadherin, which are involved in EMT, were observed in the dermal eccrine glands. These findings suggested the progression of EMT in the dermal eccrine glands in morphea.",
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Takahashi, M, Akamatsu, H, Yagami, A, Hasegawa, S, Ohgo, S, Abe, M, Iwata, Y, Arima, M, Mizutani, H, Nakata, S & Matsunaga, K 2013, 'Epithelial-mesenchymal transition of the eccrine glands is involved in skin fibrosis in morphea', Journal of Dermatology, vol. 40, no. 9, pp. 720-725. https://doi.org/10.1111/1346-8138.12235

Epithelial-mesenchymal transition of the eccrine glands is involved in skin fibrosis in morphea. / Takahashi, Masayuki; Akamatsu, Hirohiko; Yagami, Akiko; Hasegawa, Seiji; Ohgo, Shiroh; Abe, Masamichi; Iwata, Yohei; Arima, Masaru; Mizutani, Hiroshi; Nakata, Satoru; Matsunaga, Kayoko.

In: Journal of Dermatology, Vol. 40, No. 9, 01.09.2013, p. 720-725.

Research output: Contribution to journalArticle

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T1 - Epithelial-mesenchymal transition of the eccrine glands is involved in skin fibrosis in morphea

AU - Takahashi, Masayuki

AU - Akamatsu, Hirohiko

AU - Yagami, Akiko

AU - Hasegawa, Seiji

AU - Ohgo, Shiroh

AU - Abe, Masamichi

AU - Iwata, Yohei

AU - Arima, Masaru

AU - Mizutani, Hiroshi

AU - Nakata, Satoru

AU - Matsunaga, Kayoko

PY - 2013/9/1

Y1 - 2013/9/1

N2 - Morphea is a type of localized scleroderma. It is a skin disease involving the development of fibrosis in the dermis and subcutaneous fat tissue beneath without a visceral lesion, and the cause is still unclear. An involvement of epithelial-mesenchymal transition (EMT) has been reported as a cause of tissue fibrosis, but this was mostly observed in pulmonary and hepatic fibrosis, and the involvement of EMT in a skin disease, morphea, has not been studied. Thus, we analyzed the involvement of EMT in skin fibrosis in morphea patients using pathological techniques. Skin lesions of six morphea patients were analyzed (five female and one male patient). As a control, non-light-exposed skin lesions of 11 healthy females were analyzed. Concretely, tissue samples were prepared from these subjects and subjected to immunostaining of transforming growth factor (TGF)-β1, α-smooth muscle actin (α-SMA) and fibronectin, which have been reported to be associated with fibrosis, and Snail1 and E-cadherin, which are considered to be involved in EMT, and expressions of these were analyzed. In morphea patients, dermal expression of TGF-β1, α-SMA and fibronectin, which are involved in fibrosis, was enhanced, and, at the same time, enhanced expression of Snail1 and reduced expression of E-cadherin, which are involved in EMT, were observed in the dermal eccrine glands. These findings suggested the progression of EMT in the dermal eccrine glands in morphea.

AB - Morphea is a type of localized scleroderma. It is a skin disease involving the development of fibrosis in the dermis and subcutaneous fat tissue beneath without a visceral lesion, and the cause is still unclear. An involvement of epithelial-mesenchymal transition (EMT) has been reported as a cause of tissue fibrosis, but this was mostly observed in pulmonary and hepatic fibrosis, and the involvement of EMT in a skin disease, morphea, has not been studied. Thus, we analyzed the involvement of EMT in skin fibrosis in morphea patients using pathological techniques. Skin lesions of six morphea patients were analyzed (five female and one male patient). As a control, non-light-exposed skin lesions of 11 healthy females were analyzed. Concretely, tissue samples were prepared from these subjects and subjected to immunostaining of transforming growth factor (TGF)-β1, α-smooth muscle actin (α-SMA) and fibronectin, which have been reported to be associated with fibrosis, and Snail1 and E-cadherin, which are considered to be involved in EMT, and expressions of these were analyzed. In morphea patients, dermal expression of TGF-β1, α-SMA and fibronectin, which are involved in fibrosis, was enhanced, and, at the same time, enhanced expression of Snail1 and reduced expression of E-cadherin, which are involved in EMT, were observed in the dermal eccrine glands. These findings suggested the progression of EMT in the dermal eccrine glands in morphea.

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