Epstein-barr virus BBRF2 is required for maximum infectivity

H. M.Abdullah Al Masud, Yusuke Yanagi, Takahiro Watanabe, Yoshitaka Sato, Hiroshi Kimura, Takayuki Murata

Research output: Contribution to journalArticle

Abstract

Epstein-Barr virus (EBV) is a member of the gammaherpesvirinae, which causes infectious mononucleosis and several types of cancer. BBRF2 is an uncharacterized gene of EBV and is expressed during the lytic phase. To evaluate its function, BBRF2-knockout EBV was prepared using bacterial artificial chromosome (BAC) technology and the CRISPR/Cas9 system. Although viral gene expression, DNA synthesis, and progeny secretion were not affected, the infectivity of progeny viruses was significantly reduced by the disruption of BBRF2. When expressed alone, BBRF2 protein localized to the nucleus and cytoplasm, while the coexpression of an interacting partner, BSRF1, resulted in its relocalization to the cytoplasm. Interestingly, the coexpression of BBRF2 protected BSRF1 from proteasome/ubiquitin-dependent degradation. Therefore, BBRF2, together with BSRF1, augments viral infectivity.

Original languageEnglish
Article number705
JournalMicroorganisms
Volume7
Issue number12
DOIs
Publication statusPublished - 12-2019

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Human Herpesvirus 4
Gammaherpesvirinae
Cytoplasm
Clustered Regularly Interspaced Short Palindromic Repeats
Bacterial Artificial Chromosomes
Infectious Mononucleosis
Viral Genes
Proteasome Endopeptidase Complex
Ubiquitin
Viruses
Technology
Gene Expression
DNA
Genes
Neoplasms
Proteins

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Virology
  • Microbiology (medical)

Cite this

Al Masud, H. M. A., Yanagi, Y., Watanabe, T., Sato, Y., Kimura, H., & Murata, T. (2019). Epstein-barr virus BBRF2 is required for maximum infectivity. Microorganisms, 7(12), [705]. https://doi.org/10.3390/microorganisms7120705
Al Masud, H. M.Abdullah ; Yanagi, Yusuke ; Watanabe, Takahiro ; Sato, Yoshitaka ; Kimura, Hiroshi ; Murata, Takayuki. / Epstein-barr virus BBRF2 is required for maximum infectivity. In: Microorganisms. 2019 ; Vol. 7, No. 12.
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Al Masud, HMA, Yanagi, Y, Watanabe, T, Sato, Y, Kimura, H & Murata, T 2019, 'Epstein-barr virus BBRF2 is required for maximum infectivity', Microorganisms, vol. 7, no. 12, 705. https://doi.org/10.3390/microorganisms7120705

Epstein-barr virus BBRF2 is required for maximum infectivity. / Al Masud, H. M.Abdullah; Yanagi, Yusuke; Watanabe, Takahiro; Sato, Yoshitaka; Kimura, Hiroshi; Murata, Takayuki.

In: Microorganisms, Vol. 7, No. 12, 705, 12.2019.

Research output: Contribution to journalArticle

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AU - Al Masud, H. M.Abdullah

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