Epstein-barr virus BBRF2 is required for maximum infectivity

H. M.Abdullah Al Masud; Yusuke Yanagi; Takahiro Watanabe; Yoshitaka Sato; Hiroshi Kimura; Takayuki Murata

doi:10.3390/microorganisms7120705

Epstein-barr virus BBRF2 is required for maximum infectivity

H. M.Abdullah Al Masud, Yusuke Yanagi, Takahiro Watanabe, Yoshitaka Sato, Hiroshi Kimura, Takayuki Murata

Research output: Contribution to journal › Article › peer-review

13 Citations (Scopus)

Abstract

Epstein-Barr virus (EBV) is a member of the gammaherpesvirinae, which causes infectious mononucleosis and several types of cancer. BBRF2 is an uncharacterized gene of EBV and is expressed during the lytic phase. To evaluate its function, BBRF2-knockout EBV was prepared using bacterial artificial chromosome (BAC) technology and the CRISPR/Cas9 system. Although viral gene expression, DNA synthesis, and progeny secretion were not affected, the infectivity of progeny viruses was significantly reduced by the disruption of BBRF2. When expressed alone, BBRF2 protein localized to the nucleus and cytoplasm, while the coexpression of an interacting partner, BSRF1, resulted in its relocalization to the cytoplasm. Interestingly, the coexpression of BBRF2 protected BSRF1 from proteasome/ubiquitin-dependent degradation. Therefore, BBRF2, together with BSRF1, augments viral infectivity.

Original language	English
Article number	705
Journal	Microorganisms
Volume	7
Issue number	12
DOIs	https://doi.org/10.3390/microorganisms7120705
Publication status	Published - 12-2019
Externally published	Yes

All Science Journal Classification (ASJC) codes

Microbiology
Microbiology (medical)
Virology

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.3390/microorganisms7120705

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title = "Epstein-barr virus BBRF2 is required for maximum infectivity",

abstract = "Epstein-Barr virus (EBV) is a member of the gammaherpesvirinae, which causes infectious mononucleosis and several types of cancer. BBRF2 is an uncharacterized gene of EBV and is expressed during the lytic phase. To evaluate its function, BBRF2-knockout EBV was prepared using bacterial artificial chromosome (BAC) technology and the CRISPR/Cas9 system. Although viral gene expression, DNA synthesis, and progeny secretion were not affected, the infectivity of progeny viruses was significantly reduced by the disruption of BBRF2. When expressed alone, BBRF2 protein localized to the nucleus and cytoplasm, while the coexpression of an interacting partner, BSRF1, resulted in its relocalization to the cytoplasm. Interestingly, the coexpression of BBRF2 protected BSRF1 from proteasome/ubiquitin-dependent degradation. Therefore, BBRF2, together with BSRF1, augments viral infectivity.",

keywords = "BAC, BBRF2, CRISPR/Cas9, EBV, Lytic cycle",

author = "{Al Masud}, {H. M.Abdullah} and Yusuke Yanagi and Takahiro Watanabe and Yoshitaka Sato and Hiroshi Kimura and Takayuki Murata",

note = "Publisher Copyright: {\textcopyright} 2019 by the authors. Licensee MDPI, Basel, Switzerland.",

year = "2019",

month = dec,

doi = "10.3390/microorganisms7120705",

language = "English",

volume = "7",

journal = "Microorganisms",

issn = "2076-2607",

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T1 - Epstein-barr virus BBRF2 is required for maximum infectivity

AU - Al Masud, H. M.Abdullah

AU - Yanagi, Yusuke

AU - Watanabe, Takahiro

AU - Sato, Yoshitaka

AU - Kimura, Hiroshi

AU - Murata, Takayuki

PY - 2019/12

Y1 - 2019/12

N2 - Epstein-Barr virus (EBV) is a member of the gammaherpesvirinae, which causes infectious mononucleosis and several types of cancer. BBRF2 is an uncharacterized gene of EBV and is expressed during the lytic phase. To evaluate its function, BBRF2-knockout EBV was prepared using bacterial artificial chromosome (BAC) technology and the CRISPR/Cas9 system. Although viral gene expression, DNA synthesis, and progeny secretion were not affected, the infectivity of progeny viruses was significantly reduced by the disruption of BBRF2. When expressed alone, BBRF2 protein localized to the nucleus and cytoplasm, while the coexpression of an interacting partner, BSRF1, resulted in its relocalization to the cytoplasm. Interestingly, the coexpression of BBRF2 protected BSRF1 from proteasome/ubiquitin-dependent degradation. Therefore, BBRF2, together with BSRF1, augments viral infectivity.

AB - Epstein-Barr virus (EBV) is a member of the gammaherpesvirinae, which causes infectious mononucleosis and several types of cancer. BBRF2 is an uncharacterized gene of EBV and is expressed during the lytic phase. To evaluate its function, BBRF2-knockout EBV was prepared using bacterial artificial chromosome (BAC) technology and the CRISPR/Cas9 system. Although viral gene expression, DNA synthesis, and progeny secretion were not affected, the infectivity of progeny viruses was significantly reduced by the disruption of BBRF2. When expressed alone, BBRF2 protein localized to the nucleus and cytoplasm, while the coexpression of an interacting partner, BSRF1, resulted in its relocalization to the cytoplasm. Interestingly, the coexpression of BBRF2 protected BSRF1 from proteasome/ubiquitin-dependent degradation. Therefore, BBRF2, together with BSRF1, augments viral infectivity.

KW - BAC

KW - BBRF2

KW - CRISPR/Cas9

KW - EBV

KW - Lytic cycle

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DO - 10.3390/microorganisms7120705

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VL - 7

JO - Microorganisms

JF - Microorganisms

IS - 12

M1 - 705

ER -

Epstein-barr virus BBRF2 is required for maximum infectivity

Abstract

All Science Journal Classification (ASJC) codes

UN SDGs

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