Epstein-Barr virus genome packaging factors accumulate in BMRF1-cores within viral replication compartments

Atsuko Sugimoto, Yoriko Yamashita, Teru Kanda, Takayuki Murata, Tatsuya Tsurumi

Research output: Contribution to journalArticle

Abstract

Productive replication of Epstein-Barr virus (EBV) during the lytic cycle occurs in discrete sites within nuclei, termed replication compartments. We previously proposed that replication compartments consist of two subnuclear domains: "ongoing replication foci" and "BMRF1-cores". Viral genome replication takes place in ongoing replication foci, which are enriched with viral replication proteins, such as BALF5 and BALF2. Amplified DNA and BMRF1 protein accumulate in BMRF1-cores, which are surrounded by ongoing replication foci. We here determined the locations of procapsid and genome-packaging proteins of EBV via three-dimensional (3D) surface reconstruction and correlative fluorescence microscopy- electron microscopy (FM-EM). The results revealed that viral factors required for DNA packaging, such as BGLF1, BVRF1, and BFLF1 proteins, are located in the innermost subdomains of the BMRF1-cores. In contrast, capsid structural proteins, such as BBRF1, BORF1, BDLF1, and BVRF2, were found both outside and inside the BMRF1-cores. Based on these observations, we propose a model in which viral procapsids are assembled outside the BMRF1-cores and subsequently migrate therein, where viral DNA encapsidation occurs. To our knowledge, this is the first report describing capsid assembly sites in relation to EBV replication compartments.

Original languageEnglish
Article numbere0222519
JournalPloS one
Volume14
Issue number9
DOIs
Publication statusPublished - 01-01-2019

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Virus Assembly
Human herpesvirus 4
virus replication
Human Herpesvirus 4
Viruses
Packaging
Genes
Genome
DNA Packaging
Proteins
proteins
Viral Genome
Capsid
Viral DNA
Capsid Proteins
Viral Proteins
Product Packaging
Virus Replication
Fluorescence Microscopy
capsid

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)
  • General

Cite this

Sugimoto, Atsuko ; Yamashita, Yoriko ; Kanda, Teru ; Murata, Takayuki ; Tsurumi, Tatsuya. / Epstein-Barr virus genome packaging factors accumulate in BMRF1-cores within viral replication compartments. In: PloS one. 2019 ; Vol. 14, No. 9.
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Epstein-Barr virus genome packaging factors accumulate in BMRF1-cores within viral replication compartments. / Sugimoto, Atsuko; Yamashita, Yoriko; Kanda, Teru; Murata, Takayuki; Tsurumi, Tatsuya.

In: PloS one, Vol. 14, No. 9, e0222519, 01.01.2019.

Research output: Contribution to journalArticle

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