Erythrocyte spermidine as a biomarker for focal cerebral infarction in rat

Kenmei Mizutani, Kan Shimpo, Takeshi Chihara, Shigeru Sonoda

Research output: Contribution to journalArticle

Abstract

Polyamine metabolism has been studied in rat brains subjected to focal and global cerebral ischemia injury by measuring levels of the polyamines putrescine, spermidine and spermine in the brain. However, little is known about erythrocyte polyamine changes in ischemic rat. The aim of our study was to compare the sequential erythrocyte polyamine levels with brain injury volume after focal cerebral infarction following reperfusion. Middle cerebral artery was occluded for 2.5 hours in male Sprague-Dawley rats by an intraluminal suture method. The brain injury area was negatively correlated with erythrocyte spermidine levels from 48 to 96 hours after onset of the infarction event (n=14, r=-0.86, p<0.01). Time-dependent changes of erythrocyte spermidine were markedly decreased at 48, 72 and 96 hours after cerebral infarction, compared with sham-operated group or normal group. These results suggest that erythrocyte spermidine is a useful biomarker for measuring the infarct volume after middle cerebral artery occlusion following reperfusion in rat.

Original languageEnglish
Pages (from-to)169-176
Number of pages8
JournalBiogenic Amines
Volume21
Issue number3
Publication statusPublished - 01-12-2007

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Spermidine
Cerebral Infarction
Polyamines
Erythrocytes
Biomarkers
Brain Injuries
Reperfusion
Putrescine
Spermine
Middle Cerebral Artery Infarction
Middle Cerebral Artery
Brain
Brain Ischemia
Infarction
Sutures
Sprague Dawley Rats
Wounds and Injuries

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)
  • Pharmacology

Cite this

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Erythrocyte spermidine as a biomarker for focal cerebral infarction in rat. / Mizutani, Kenmei; Shimpo, Kan; Chihara, Takeshi; Sonoda, Shigeru.

In: Biogenic Amines, Vol. 21, No. 3, 01.12.2007, p. 169-176.

Research output: Contribution to journalArticle

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AU - Shimpo, Kan

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