Escherichia coli Producing CTX-M-2 β-Lactamase in Cattle, Japan

Yutaka Shiraki, Naohiro Shibata, Yohei Doi, Yoshichika Arakawa

Research output: Contribution to journalArticle

92 Citations (Scopus)

Abstract

From November 2000 to June 2001, Escherichia coli strains producing CTX-M-2 β-lactamase were isolated from 6 (1.5%) of 396 cattle fecal samples and 2 (0.7%) of 270 surface swabs of cattle carcasses in Japan. The blaCTX-M-2 gene responsible for CTX-M-2 production was encoded on transferable plasmids, and the gene was transferred to E. coli CSH2 with a very high frequency (2 × 10-4 to 6 × 10-1 per donor cells) by conjugation. Random amplified polymorphic DNA analysis of nine isolates showed at least five different patterns. These findings suggest that CTX-M-2 producers might have originated from cattle through the use of cephalosporins such as ceftiofur and that cattle could be a reservoir of CTX-M-2-producing E. coli. Continuous and strategic surveillance of antimicrobial-resistant bacteria in livestock is essential to suppress further dissemination of these bacteria into society at large.

Original languageEnglish
Pages (from-to)69-75
Number of pages7
JournalEmerging Infectious Diseases
Volume10
Issue number1
DOIs
Publication statusPublished - 01-01-2004

Fingerprint

Japan
Escherichia coli
Bacteria
Livestock
Cephalosporins
Genes
Plasmids
beta-lactamase CTX-2
DNA

All Science Journal Classification (ASJC) codes

  • Epidemiology
  • Microbiology (medical)
  • Infectious Diseases

Cite this

Shiraki, Yutaka ; Shibata, Naohiro ; Doi, Yohei ; Arakawa, Yoshichika. / Escherichia coli Producing CTX-M-2 β-Lactamase in Cattle, Japan. In: Emerging Infectious Diseases. 2004 ; Vol. 10, No. 1. pp. 69-75.
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Escherichia coli Producing CTX-M-2 β-Lactamase in Cattle, Japan. / Shiraki, Yutaka; Shibata, Naohiro; Doi, Yohei; Arakawa, Yoshichika.

In: Emerging Infectious Diseases, Vol. 10, No. 1, 01.01.2004, p. 69-75.

Research output: Contribution to journalArticle

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