Establishment and characterization of a novel cell line, TK-6, derived from T cell blast crisis of chronic myelogenous leukemia, with the secretion of parathyroid hormone-related protein

T. Watanabe, T. Kataoka, S. Mizuta, M. Kobayashi, T. Uchida, K. Imai, H. Wada, T. Kinoshita, T. Murate, S. Mizutani, H. Saito, T. Hotta

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12 Citations (Scopus)

Abstract

We established a novel T cell line, designated TK-6, from a patient with T cell lineage blast crisis of chronic myelogenous leukemia (CML) complicated by hypercalcemia. A surface marker study showed T cell phenotype, cluster designation (CD)4, CD5 and CD7. Light and electron microscopic examination revealed myeloperoxidase (MPO)-negative, however, ultrastructural examination under certain specific conditions demonstrated that some cells were MPO-positive. The TK-6 cell karyotype carried a t(9;22)(q34;q11) and additional chromosome aberrations, including a deletion of the long arm of chromosome 6 and the abnormality of chromosome 7. Southern blot analysis showed rearrangement of the T cell receptor β-chain (TCRβ) gene and the major breakpoint cluster region (bcr) gene. Northern blot analysis detected the expression of the parathyroid hormone-related protein (PTHrP) gene, however, the proviral genome of human T cell leukemia virus type I (HTLV-I) was negative. This cell line will provide a valuable resource for the analysis of the relationship between T cell lineage crisis and myeloid differentiation and for the analysis of humoral hypercalcemia of malignancy (HHM) or leukemia.

Original languageEnglish
Pages (from-to)1926-1934
Number of pages9
JournalLeukemia
Volume9
Issue number11
Publication statusPublished - 11-1995

All Science Journal Classification (ASJC) codes

  • Hematology
  • Oncology
  • Cancer Research

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