Establishment of a monitoring system to detect inhibition of mRNA processing

Ken Ichi Fujita; Masumi Okamura; Sachiko Nishimoto; Tomoya Kurihara; Keiko Momma; Yusaku Miyamae; Taiho Kambe; Masaya Nagao; Hiroshi Narita; Seiji Masuda

doi:10.1271/bbb.120226

Establishment of a monitoring system to detect inhibition of mRNA processing

Ken Ichi Fujita
, Masumi Okamura
, Sachiko Nishimoto
, Tomoya Kurihara
, Keiko Momma
, Yusaku Miyamae
, Taiho Kambe
, Masaya Nagao
, Hiroshi Narita
, Seiji Masuda

Research output: Contribution to journal › Article › peer-review

6 Citations (Scopus)

Abstract

A number of proteins complete mRNA processing in the nucleus, thus, inhibitor of mRNA processing is worth finding to analyze the mechanism of mRNA maturation in detail. Here, we established a monitoring system for mRNA processing using a test compound, spliceostatin A (SSA), which inhibits mRNA splicing. This system should serve to facilitate the discovery of novel compounds from natural resources that inhibit mRNA processing.

Original language	English
Pages (from-to)	1248-1251
Number of pages	4
Journal	Bioscience, Biotechnology and Biochemistry
Volume	76
Issue number	6
DOIs	https://doi.org/10.1271/bbb.120226
Publication status	Published - 2012
Externally published	Yes

All Science Journal Classification (ASJC) codes

General Medicine

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10.1271/bbb.120226

Cite this

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title = "Establishment of a monitoring system to detect inhibition of mRNA processing",

abstract = "A number of proteins complete mRNA processing in the nucleus, thus, inhibitor of mRNA processing is worth finding to analyze the mechanism of mRNA maturation in detail. Here, we established a monitoring system for mRNA processing using a test compound, spliceostatin A (SSA), which inhibits mRNA splicing. This system should serve to facilitate the discovery of novel compounds from natural resources that inhibit mRNA processing.",

keywords = "Inhibitor, Luciferase reporter, MRNA processing, RNA-fluorescence in situ hybridyzation (FISH), Reverse transcription-polymerase chain reaction (RT-PCR)",

author = "Fujita, \{Ken Ichi\} and Masumi Okamura and Sachiko Nishimoto and Tomoya Kurihara and Keiko Momma and Yusaku Miyamae and Taiho Kambe and Masaya Nagao and Hiroshi Narita and Seiji Masuda",

note = "Funding Information: We thank Dr. M. Yoshida for kindly providing SSA. This work was supported in part by Grants-in-Aid from the Central Miso Research Institute, JST in research for adaptable and seamless technology transfer program through target-driven R\&D, exploratory research stage, and the Kieikai Research Foundation to S. M.",

year = "2012",

doi = "10.1271/bbb.120226",

language = "English",

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pages = "1248--1251",

journal = "Bioscience, Biotechnology and Biochemistry",

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T1 - Establishment of a monitoring system to detect inhibition of mRNA processing

AU - Fujita, Ken Ichi

AU - Okamura, Masumi

AU - Nishimoto, Sachiko

AU - Kurihara, Tomoya

AU - Momma, Keiko

AU - Miyamae, Yusaku

AU - Kambe, Taiho

AU - Nagao, Masaya

AU - Narita, Hiroshi

AU - Masuda, Seiji

N1 - Funding Information: We thank Dr. M. Yoshida for kindly providing SSA. This work was supported in part by Grants-in-Aid from the Central Miso Research Institute, JST in research for adaptable and seamless technology transfer program through target-driven R&D, exploratory research stage, and the Kieikai Research Foundation to S. M.

PY - 2012

Y1 - 2012

N2 - A number of proteins complete mRNA processing in the nucleus, thus, inhibitor of mRNA processing is worth finding to analyze the mechanism of mRNA maturation in detail. Here, we established a monitoring system for mRNA processing using a test compound, spliceostatin A (SSA), which inhibits mRNA splicing. This system should serve to facilitate the discovery of novel compounds from natural resources that inhibit mRNA processing.

AB - A number of proteins complete mRNA processing in the nucleus, thus, inhibitor of mRNA processing is worth finding to analyze the mechanism of mRNA maturation in detail. Here, we established a monitoring system for mRNA processing using a test compound, spliceostatin A (SSA), which inhibits mRNA splicing. This system should serve to facilitate the discovery of novel compounds from natural resources that inhibit mRNA processing.

KW - Inhibitor

KW - Luciferase reporter

KW - MRNA processing

KW - RNA-fluorescence in situ hybridyzation (FISH)

KW - Reverse transcription-polymerase chain reaction (RT-PCR)

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UR - https://www.scopus.com/pages/publications/84862748839#tab=citedBy

U2 - 10.1271/bbb.120226

DO - 10.1271/bbb.120226

M3 - Article

C2 - 22790958

AN - SCOPUS:84862748839

SN - 0916-8451

VL - 76

SP - 1248

EP - 1251

JO - Bioscience, Biotechnology and Biochemistry

JF - Bioscience, Biotechnology and Biochemistry

IS - 6

ER -

Establishment of a monitoring system to detect inhibition of mRNA processing

Abstract

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