Establishment of a new cell line of endometrioid carcinoma of the ovary and its chemosensitivity

Tomokazu Umezu; Hiroaki Kajiyama; Mikio Terauchi; Kiyosumi Shibata; Kazuhiko Ino; Akihiro Nawa; Fumitaka Kikkawa

doi:10.1111/j.1749-0774.2007.00030.x

Establishment of a new cell line of endometrioid carcinoma of the ovary and its chemosensitivity

Tomokazu Umezu, Hiroaki Kajiyama, Mikio Terauchi, Kiyosumi Shibata, Kazuhiko Ino, Akihiro Nawa, Fumitaka Kikkawa

Research output: Contribution to journal › Article › peer-review

10 Citations (Scopus)

Abstract

A new cell line (NOE) of human ovarian endometrioid carcinoma was established and characterized. The cell line showed a short spindle-shaped morphology and continued to grow for more than 4 months without contact inhibition. The doubling time was approximately 15.5 h at the 10th passage. The chromosome number was aneuploid. The IC50 values of paclitaxel, cisplatin and carboplatin were 26.4 ng/mL, 2.4 μg/mL and 32.5 μg/mL, respectively. The NOE cells expressed estrogen receptor α. In nude mice, we confirmed tumor formation of NOE cells. These results indicated that NOE cells showed similar chemosensitivity and properties to those of the original tumor and might be useful in basic studies on the diagnosis, treatment and etiology of ovarian tumors.

Original language	English
Pages (from-to)	71-76
Number of pages	6
Journal	Human Cell
Volume	20
Issue number	3
DOIs	https://doi.org/10.1111/j.1749-0774.2007.00030.x
Publication status	Published - 08-2007
Externally published	Yes

All Science Journal Classification (ASJC) codes

Cell Biology
Cancer Research

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1111/j.1749-0774.2007.00030.x

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abstract = "A new cell line (NOE) of human ovarian endometrioid carcinoma was established and characterized. The cell line showed a short spindle-shaped morphology and continued to grow for more than 4 months without contact inhibition. The doubling time was approximately 15.5 h at the 10th passage. The chromosome number was aneuploid. The IC50 values of paclitaxel, cisplatin and carboplatin were 26.4 ng/mL, 2.4 μg/mL and 32.5 μg/mL, respectively. The NOE cells expressed estrogen receptor α. In nude mice, we confirmed tumor formation of NOE cells. These results indicated that NOE cells showed similar chemosensitivity and properties to those of the original tumor and might be useful in basic studies on the diagnosis, treatment and etiology of ovarian tumors.",

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AU - Umezu, Tomokazu

AU - Kajiyama, Hiroaki

AU - Terauchi, Mikio

AU - Shibata, Kiyosumi

AU - Ino, Kazuhiko

AU - Nawa, Akihiro

AU - Kikkawa, Fumitaka

PY - 2007/8

Y1 - 2007/8

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AB - A new cell line (NOE) of human ovarian endometrioid carcinoma was established and characterized. The cell line showed a short spindle-shaped morphology and continued to grow for more than 4 months without contact inhibition. The doubling time was approximately 15.5 h at the 10th passage. The chromosome number was aneuploid. The IC50 values of paclitaxel, cisplatin and carboplatin were 26.4 ng/mL, 2.4 μg/mL and 32.5 μg/mL, respectively. The NOE cells expressed estrogen receptor α. In nude mice, we confirmed tumor formation of NOE cells. These results indicated that NOE cells showed similar chemosensitivity and properties to those of the original tumor and might be useful in basic studies on the diagnosis, treatment and etiology of ovarian tumors.

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Establishment of a new cell line of endometrioid carcinoma of the ovary and its chemosensitivity

Abstract

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