Establishment of a new immortalized human corneal epithelial cell line (iHCE-NY1) for use in evaluating eye irritancy by in vitro test methods

Naoki Yamamoto, Yoshinao Kato, Atsushi Sato, Noriko Hiramatsu, Hiromi Yamashita, Mahito Ohkuma, Ei ichi Miyachi, Masayuki Horiguchi, Koji Hirano, Hajime Kojima

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Abstract

In vitro test methods that use human corneal epithelial cells to evaluate the eye irritation potency of chemical substances do not use human corneal epithelium because it has been difficult to maintain more than four passages. In this study, we make a new cell line comprising immortalized human corneal epithelial cells (iHCE-NY1). The IC50 of iHCE-NY1 cells is slightly higher than that of Statens Seruminstitut Rabbit Cornea (SIRC) cells, which are currently used in some in vitro test methods. CDKN1A in iHCE-NY1 cells was used as a marker of gene expression to indicate cell cycle activity. This enabled us to evaluate cell recovery characteristics at concentrations lower than the IC50 of cytotoxic tests.

Original languageEnglish
Pages (from-to)742-748
Number of pages7
JournalIn Vitro Cellular and Developmental Biology - Animal
Volume52
Issue number7
DOIs
Publication statusPublished - 01-08-2016

All Science Journal Classification (ASJC) codes

  • Developmental Biology
  • Cell Biology

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    Yamamoto, N., Kato, Y., Sato, A., Hiramatsu, N., Yamashita, H., Ohkuma, M., Miyachi, E. I., Horiguchi, M., Hirano, K., & Kojima, H. (2016). Establishment of a new immortalized human corneal epithelial cell line (iHCE-NY1) for use in evaluating eye irritancy by in vitro test methods. In Vitro Cellular and Developmental Biology - Animal, 52(7), 742-748. https://doi.org/10.1007/s11626-016-0038-9