Abstract
In vitro test methods that use human corneal epithelial cells to evaluate the eye irritation potency of chemical substances do not use human corneal epithelium because it has been difficult to maintain more than four passages. In this study, we make a new cell line comprising immortalized human corneal epithelial cells (iHCE-NY1). The IC50 of iHCE-NY1 cells is slightly higher than that of Statens Seruminstitut Rabbit Cornea (SIRC) cells, which are currently used in some in vitro test methods. CDKN1A in iHCE-NY1 cells was used as a marker of gene expression to indicate cell cycle activity. This enabled us to evaluate cell recovery characteristics at concentrations lower than the IC50 of cytotoxic tests.
| Original language | English |
|---|---|
| Pages (from-to) | 742-748 |
| Number of pages | 7 |
| Journal | In Vitro Cellular and Developmental Biology - Animal |
| Volume | 52 |
| Issue number | 7 |
| DOIs | |
| Publication status | Published - 01-08-2016 |
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All Science Journal Classification (ASJC) codes
- Developmental Biology
- Cell Biology
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Establishment of a new immortalized human corneal epithelial cell line (iHCE-NY1) for use in evaluating eye irritancy by in vitro test methods. / Yamamoto, Naoki; Kato, Yoshinao; Sato, Atsushi; Hiramatsu, Noriko; Yamashita, Hiromi; Ohkuma, Mahito; Miyachi, Ei ichi; Horiguchi, Masayuki; Hirano, Koji; Kojima, Hajime.
In: In Vitro Cellular and Developmental Biology - Animal, Vol. 52, No. 7, 01.08.2016, p. 742-748.Research output: Contribution to journal › Article
TY - JOUR
T1 - Establishment of a new immortalized human corneal epithelial cell line (iHCE-NY1) for use in evaluating eye irritancy by in vitro test methods
AU - Yamamoto, Naoki
AU - Kato, Yoshinao
AU - Sato, Atsushi
AU - Hiramatsu, Noriko
AU - Yamashita, Hiromi
AU - Ohkuma, Mahito
AU - Miyachi, Ei ichi
AU - Horiguchi, Masayuki
AU - Hirano, Koji
AU - Kojima, Hajime
PY - 2016/8/1
Y1 - 2016/8/1
N2 - In vitro test methods that use human corneal epithelial cells to evaluate the eye irritation potency of chemical substances do not use human corneal epithelium because it has been difficult to maintain more than four passages. In this study, we make a new cell line comprising immortalized human corneal epithelial cells (iHCE-NY1). The IC50 of iHCE-NY1 cells is slightly higher than that of Statens Seruminstitut Rabbit Cornea (SIRC) cells, which are currently used in some in vitro test methods. CDKN1A in iHCE-NY1 cells was used as a marker of gene expression to indicate cell cycle activity. This enabled us to evaluate cell recovery characteristics at concentrations lower than the IC50 of cytotoxic tests.
AB - In vitro test methods that use human corneal epithelial cells to evaluate the eye irritation potency of chemical substances do not use human corneal epithelium because it has been difficult to maintain more than four passages. In this study, we make a new cell line comprising immortalized human corneal epithelial cells (iHCE-NY1). The IC50 of iHCE-NY1 cells is slightly higher than that of Statens Seruminstitut Rabbit Cornea (SIRC) cells, which are currently used in some in vitro test methods. CDKN1A in iHCE-NY1 cells was used as a marker of gene expression to indicate cell cycle activity. This enabled us to evaluate cell recovery characteristics at concentrations lower than the IC50 of cytotoxic tests.
UR - http://www.scopus.com/inward/record.url?scp=84964582957&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84964582957&partnerID=8YFLogxK
U2 - 10.1007/s11626-016-0038-9
DO - 10.1007/s11626-016-0038-9
M3 - Article
VL - 52
SP - 742
EP - 748
JO - In Vitro Cellular and Developmental Biology - Animal
JF - In Vitro Cellular and Developmental Biology - Animal
SN - 1071-2690
IS - 7
ER -