Establishment of a reverse genetics system for rotavirus

Satoshi Komoto; Koki Taniguchi

doi:10.2222/jsv.56.183

Establishment of a reverse genetics system for rotavirus

Satoshi Komoto, Koki Taniguchi

Virology

Research output: Contribution to journal › Review article › peer-review

1 Citation (Scopus)

Abstract

The rotavirus genome is composed of 11 segments of double-stranded RNA (dsRNA). Rotavirus is the leading etiological agent of severe gastroenteritis in infants and young children worldwide. Reverse genetics is the powerful and ideal methodology for the molecular study of virus replication, which enables the virus genome to be artificially manipulated. Very recently, we developed the first reverse genetics system for rotavirus, which enables one to generate an infectious rotavirus containing a novel gene segment derived from cDNA. In this review, we describe each steps of rotavirus replication to understand the background to the establishment of a reverse genetics system for rotavirus, and summarize the reverse genetics systems for segmented dsRNA viruses including rotavirus.

Original language	English
Pages (from-to)	183-192
Number of pages	10
Journal	Uirusu. Journal of virology
Volume	56
Issue number	2
DOIs	https://doi.org/10.2222/jsv.56.183
Publication status	Published - 12-2006

All Science Journal Classification (ASJC) codes

General Medicine

Access to Document

10.2222/jsv.56.183

Cite this

@article{d04e76d5d48c44539af1919eb6c77293,

title = "Establishment of a reverse genetics system for rotavirus",

abstract = "The rotavirus genome is composed of 11 segments of double-stranded RNA (dsRNA). Rotavirus is the leading etiological agent of severe gastroenteritis in infants and young children worldwide. Reverse genetics is the powerful and ideal methodology for the molecular study of virus replication, which enables the virus genome to be artificially manipulated. Very recently, we developed the first reverse genetics system for rotavirus, which enables one to generate an infectious rotavirus containing a novel gene segment derived from cDNA. In this review, we describe each steps of rotavirus replication to understand the background to the establishment of a reverse genetics system for rotavirus, and summarize the reverse genetics systems for segmented dsRNA viruses including rotavirus.",

author = "Satoshi Komoto and Koki Taniguchi",

note = "Copyright: This record is sourced from MEDLINE{\textregistered}/PubMed{\textregistered}, a database of the U.S. National Library of Medicine",

year = "2006",

month = dec,

doi = "10.2222/jsv.56.183",

language = "English",

volume = "56",

pages = "183--192",

journal = "Uirusu. Journal of virology",

issn = "0042-6857",

publisher = "Nihon Uirusu Gakkal/Japanese Society for Virology",

number = "2",

}

TY - JOUR

T1 - Establishment of a reverse genetics system for rotavirus

AU - Komoto, Satoshi

AU - Taniguchi, Koki

N1 - Copyright: This record is sourced from MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

PY - 2006/12

Y1 - 2006/12

N2 - The rotavirus genome is composed of 11 segments of double-stranded RNA (dsRNA). Rotavirus is the leading etiological agent of severe gastroenteritis in infants and young children worldwide. Reverse genetics is the powerful and ideal methodology for the molecular study of virus replication, which enables the virus genome to be artificially manipulated. Very recently, we developed the first reverse genetics system for rotavirus, which enables one to generate an infectious rotavirus containing a novel gene segment derived from cDNA. In this review, we describe each steps of rotavirus replication to understand the background to the establishment of a reverse genetics system for rotavirus, and summarize the reverse genetics systems for segmented dsRNA viruses including rotavirus.

AB - The rotavirus genome is composed of 11 segments of double-stranded RNA (dsRNA). Rotavirus is the leading etiological agent of severe gastroenteritis in infants and young children worldwide. Reverse genetics is the powerful and ideal methodology for the molecular study of virus replication, which enables the virus genome to be artificially manipulated. Very recently, we developed the first reverse genetics system for rotavirus, which enables one to generate an infectious rotavirus containing a novel gene segment derived from cDNA. In this review, we describe each steps of rotavirus replication to understand the background to the establishment of a reverse genetics system for rotavirus, and summarize the reverse genetics systems for segmented dsRNA viruses including rotavirus.

UR - http://www.scopus.com/inward/record.url?scp=34447271746&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=34447271746&partnerID=8YFLogxK

U2 - 10.2222/jsv.56.183

DO - 10.2222/jsv.56.183

M3 - Review article

C2 - 17446667

AN - SCOPUS:34447271746

SN - 0042-6857

VL - 56

SP - 183

EP - 192

JO - Uirusu. Journal of virology

JF - Uirusu. Journal of virology

IS - 2

ER -

Establishment of a reverse genetics system for rotavirus

Abstract

All Science Journal Classification (ASJC) codes

Access to Document

Other files and links

Fingerprint

Cite this