Establishment of an ELISA to detect anti-glycyl-tRNA synthetase antibody (anti-EJ), a serological marker of dermatomyositis/polymyositis and interstitial lung disease

Hiroaki Hane, Yoshinao Muro, Kanako Watanabe, Yasushi Ogawa, Kazumitsu Sugiura, Masashi Akiyama

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Background: The aminoacyl transfer RNA synthetases (ARSs) are a group of enzymes that charge amino acids to the cognate transfer RNA during the translation process. Previous reports demonstrated autoantibodies to 8 different ARS. Although anti-glycyl-tRNA synthetase antibodies (anti-EJ) are mainly found in patients with inflammatory myopathy, information on their clinical significances is limited, partly due to a lack of commercially available tests. Methods: We developed an ELISA and immunoprecipitation method by using recombinant EJ protein to detect the anti-EJ of 453 patients with various autoimmune connective tissue diseases (ACTDs). We also studied the influence of 3 cytokines-IL-1β, IFN-γ and IFN-α-on the level of EJ mRNA and protein expressed by human fetal lung fibroblasts. Results: Five patients were positive for anti-EJ. Although 3 of these patients had dermatomyositis/polymyositis, the other 2 patients did not have myositis. The three patients with high levels of anti-EJ antibodies in ELISA were complicated with interstitial lung disease. There was no significant change in the level of EJ protein expressed by human fetal lung fibroblasts stimulated by the cytokines. Conclusion: We developed an ELISA to detect anti-EJ by using recombinant protein. This easy-to-use ELISA could help clarify the clinical significance of anti-EJ in ACTDs.

Original languageEnglish
Pages (from-to)9-14
Number of pages6
JournalClinica Chimica Acta
Volume431
DOIs
Publication statusPublished - 20-04-2014
Externally publishedYes

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Glycine-tRNA Ligase
Amino Acyl-tRNA Synthetases
Pulmonary diseases
Dermatomyositis
Interstitial Lung Diseases
Fibroblasts
Anti-Idiotypic Antibodies
Enzyme-Linked Immunosorbent Assay
Tissue
Cytokines
Recombinant proteins
Transfer RNA
Interleukin-1
Recombinant Proteins
Autoantibodies
Proteins
Myositis
Connective Tissue Diseases
Amino Acids
Messenger RNA

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Clinical Biochemistry
  • Biochemistry, medical

Cite this

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title = "Establishment of an ELISA to detect anti-glycyl-tRNA synthetase antibody (anti-EJ), a serological marker of dermatomyositis/polymyositis and interstitial lung disease",
abstract = "Background: The aminoacyl transfer RNA synthetases (ARSs) are a group of enzymes that charge amino acids to the cognate transfer RNA during the translation process. Previous reports demonstrated autoantibodies to 8 different ARS. Although anti-glycyl-tRNA synthetase antibodies (anti-EJ) are mainly found in patients with inflammatory myopathy, information on their clinical significances is limited, partly due to a lack of commercially available tests. Methods: We developed an ELISA and immunoprecipitation method by using recombinant EJ protein to detect the anti-EJ of 453 patients with various autoimmune connective tissue diseases (ACTDs). We also studied the influence of 3 cytokines-IL-1β, IFN-γ and IFN-α-on the level of EJ mRNA and protein expressed by human fetal lung fibroblasts. Results: Five patients were positive for anti-EJ. Although 3 of these patients had dermatomyositis/polymyositis, the other 2 patients did not have myositis. The three patients with high levels of anti-EJ antibodies in ELISA were complicated with interstitial lung disease. There was no significant change in the level of EJ protein expressed by human fetal lung fibroblasts stimulated by the cytokines. Conclusion: We developed an ELISA to detect anti-EJ by using recombinant protein. This easy-to-use ELISA could help clarify the clinical significance of anti-EJ in ACTDs.",
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Establishment of an ELISA to detect anti-glycyl-tRNA synthetase antibody (anti-EJ), a serological marker of dermatomyositis/polymyositis and interstitial lung disease. / Hane, Hiroaki; Muro, Yoshinao; Watanabe, Kanako; Ogawa, Yasushi; Sugiura, Kazumitsu; Akiyama, Masashi.

In: Clinica Chimica Acta, Vol. 431, 20.04.2014, p. 9-14.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Establishment of an ELISA to detect anti-glycyl-tRNA synthetase antibody (anti-EJ), a serological marker of dermatomyositis/polymyositis and interstitial lung disease

AU - Hane, Hiroaki

AU - Muro, Yoshinao

AU - Watanabe, Kanako

AU - Ogawa, Yasushi

AU - Sugiura, Kazumitsu

AU - Akiyama, Masashi

PY - 2014/4/20

Y1 - 2014/4/20

N2 - Background: The aminoacyl transfer RNA synthetases (ARSs) are a group of enzymes that charge amino acids to the cognate transfer RNA during the translation process. Previous reports demonstrated autoantibodies to 8 different ARS. Although anti-glycyl-tRNA synthetase antibodies (anti-EJ) are mainly found in patients with inflammatory myopathy, information on their clinical significances is limited, partly due to a lack of commercially available tests. Methods: We developed an ELISA and immunoprecipitation method by using recombinant EJ protein to detect the anti-EJ of 453 patients with various autoimmune connective tissue diseases (ACTDs). We also studied the influence of 3 cytokines-IL-1β, IFN-γ and IFN-α-on the level of EJ mRNA and protein expressed by human fetal lung fibroblasts. Results: Five patients were positive for anti-EJ. Although 3 of these patients had dermatomyositis/polymyositis, the other 2 patients did not have myositis. The three patients with high levels of anti-EJ antibodies in ELISA were complicated with interstitial lung disease. There was no significant change in the level of EJ protein expressed by human fetal lung fibroblasts stimulated by the cytokines. Conclusion: We developed an ELISA to detect anti-EJ by using recombinant protein. This easy-to-use ELISA could help clarify the clinical significance of anti-EJ in ACTDs.

AB - Background: The aminoacyl transfer RNA synthetases (ARSs) are a group of enzymes that charge amino acids to the cognate transfer RNA during the translation process. Previous reports demonstrated autoantibodies to 8 different ARS. Although anti-glycyl-tRNA synthetase antibodies (anti-EJ) are mainly found in patients with inflammatory myopathy, information on their clinical significances is limited, partly due to a lack of commercially available tests. Methods: We developed an ELISA and immunoprecipitation method by using recombinant EJ protein to detect the anti-EJ of 453 patients with various autoimmune connective tissue diseases (ACTDs). We also studied the influence of 3 cytokines-IL-1β, IFN-γ and IFN-α-on the level of EJ mRNA and protein expressed by human fetal lung fibroblasts. Results: Five patients were positive for anti-EJ. Although 3 of these patients had dermatomyositis/polymyositis, the other 2 patients did not have myositis. The three patients with high levels of anti-EJ antibodies in ELISA were complicated with interstitial lung disease. There was no significant change in the level of EJ protein expressed by human fetal lung fibroblasts stimulated by the cytokines. Conclusion: We developed an ELISA to detect anti-EJ by using recombinant protein. This easy-to-use ELISA could help clarify the clinical significance of anti-EJ in ACTDs.

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