Establishment of the culture model system that reflects the process of terminal differentiation of connective tissue-type mast cells

Hirotsugu Takano; Shunsuke Nakazawa; Yasushi Okuno; Naritoshi Shirata; Sohken Tsuchiya; Takayuki Kainoh; Seigoh Takamatsu; Kazuyuki Furuta; Yoshitaka Taketomi; Yuko Naito; Hiromu Takematsu; Yasunori Kozutsumi; Gozoh Tsujimoto; Makoto Murakami; Ichiro Kudo; Atsushi Ichikawa; Kazuhisa Nakayama; Yukihiko Sugimoto; Satoshi Tanaka

doi:10.1016/j.febslet.2008.03.033

Establishment of the culture model system that reflects the process of terminal differentiation of connective tissue-type mast cells

Hirotsugu Takano, Shunsuke Nakazawa, Yasushi Okuno, Naritoshi Shirata, Sohken Tsuchiya, Takayuki Kainoh, Seigoh Takamatsu, Kazuyuki Furuta, Yoshitaka Taketomi, Yuko Naito, Hiromu Takematsu, Yasunori Kozutsumi, Gozoh Tsujimoto, Makoto Murakami, Ichiro Kudo, Atsushi Ichikawa, Kazuhisa Nakayama, Yukihiko Sugimoto, Satoshi Tanaka

Molecular Cell Biology

Research output: Contribution to journal › Article › peer-review

39 Citations (Scopus)

Abstract

To understand physiological roles of tissue mast cells, we established a culture system where bone marrow-derived immature mast cells differentiate into the connective tissue-type mast cell (CTMC)-like cells through modifying the previous co-culture system with Swiss 3T3 fibroblasts. Our system was found to reproducibly mimic the differentiation of CTMCs on the basis of several criteria, such as granule maturation and sensitivity to cationic secretagogues. The gene expression profile obtained by the microarray analyses was found to reflect many aspects of the differentiation. Our system is thus helpful to gain deeper insights into terminal differentiation of CTMCs.

Original language	English
Pages (from-to)	1444-1450
Number of pages	7
Journal	FEBS Letters
Volume	582
Issue number	10
DOIs	https://doi.org/10.1016/j.febslet.2008.03.033
Publication status	Published - 30-04-2008

All Science Journal Classification (ASJC) codes

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

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Takano, H., Nakazawa, S., Okuno, Y., Shirata, N., Tsuchiya, S., Kainoh, T., Takamatsu, S., Furuta, K., Taketomi, Y., Naito, Y., Takematsu, H., Kozutsumi, Y., Tsujimoto, G., Murakami, M., Kudo, I., Ichikawa, A., Nakayama, K., Sugimoto, Y., & Tanaka, S. (2008). Establishment of the culture model system that reflects the process of terminal differentiation of connective tissue-type mast cells. FEBS Letters, 582(10), 1444-1450. https://doi.org/10.1016/j.febslet.2008.03.033

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title = "Establishment of the culture model system that reflects the process of terminal differentiation of connective tissue-type mast cells",

abstract = "To understand physiological roles of tissue mast cells, we established a culture system where bone marrow-derived immature mast cells differentiate into the connective tissue-type mast cell (CTMC)-like cells through modifying the previous co-culture system with Swiss 3T3 fibroblasts. Our system was found to reproducibly mimic the differentiation of CTMCs on the basis of several criteria, such as granule maturation and sensitivity to cationic secretagogues. The gene expression profile obtained by the microarray analyses was found to reflect many aspects of the differentiation. Our system is thus helpful to gain deeper insights into terminal differentiation of CTMCs.",

author = "Hirotsugu Takano and Shunsuke Nakazawa and Yasushi Okuno and Naritoshi Shirata and Sohken Tsuchiya and Takayuki Kainoh and Seigoh Takamatsu and Kazuyuki Furuta and Yoshitaka Taketomi and Yuko Naito and Hiromu Takematsu and Yasunori Kozutsumi and Gozoh Tsujimoto and Makoto Murakami and Ichiro Kudo and Atsushi Ichikawa and Kazuhisa Nakayama and Yukihiko Sugimoto and Satoshi Tanaka",

note = "Funding Information: This study was supported in part by 21st Century COE Program “Knowledge Information Infrastructure for Genome Science”, a grant from Uehara Memorial Foundation, Naito Foundation, Kao Foundation for Arts and Sciences, Sankyo Foundation of Life Science, and by Grants-in-Aid for Scientific Research from the Ministry of Education, Culture, Science, Sports and Technology of Japan and from the Ministry of Health and Labor of Japan. ",

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doi = "10.1016/j.febslet.2008.03.033",

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Takano, H, Nakazawa, S, Okuno, Y, Shirata, N, Tsuchiya, S, Kainoh, T, Takamatsu, S, Furuta, K, Taketomi, Y, Naito, Y, Takematsu, H, Kozutsumi, Y, Tsujimoto, G, Murakami, M, Kudo, I, Ichikawa, A, Nakayama, K, Sugimoto, Y & Tanaka, S 2008, 'Establishment of the culture model system that reflects the process of terminal differentiation of connective tissue-type mast cells', FEBS Letters, vol. 582, no. 10, pp. 1444-1450. https://doi.org/10.1016/j.febslet.2008.03.033

TY - JOUR

T1 - Establishment of the culture model system that reflects the process of terminal differentiation of connective tissue-type mast cells

AU - Takano, Hirotsugu

AU - Nakazawa, Shunsuke

AU - Okuno, Yasushi

AU - Shirata, Naritoshi

AU - Tsuchiya, Sohken

AU - Kainoh, Takayuki

AU - Takamatsu, Seigoh

AU - Furuta, Kazuyuki

AU - Taketomi, Yoshitaka

AU - Naito, Yuko

AU - Takematsu, Hiromu

AU - Kozutsumi, Yasunori

AU - Tsujimoto, Gozoh

AU - Murakami, Makoto

AU - Kudo, Ichiro

AU - Ichikawa, Atsushi

AU - Nakayama, Kazuhisa

AU - Sugimoto, Yukihiko

AU - Tanaka, Satoshi

N1 - Funding Information: This study was supported in part by 21st Century COE Program “Knowledge Information Infrastructure for Genome Science”, a grant from Uehara Memorial Foundation, Naito Foundation, Kao Foundation for Arts and Sciences, Sankyo Foundation of Life Science, and by Grants-in-Aid for Scientific Research from the Ministry of Education, Culture, Science, Sports and Technology of Japan and from the Ministry of Health and Labor of Japan.

PY - 2008/4/30

Y1 - 2008/4/30

N2 - To understand physiological roles of tissue mast cells, we established a culture system where bone marrow-derived immature mast cells differentiate into the connective tissue-type mast cell (CTMC)-like cells through modifying the previous co-culture system with Swiss 3T3 fibroblasts. Our system was found to reproducibly mimic the differentiation of CTMCs on the basis of several criteria, such as granule maturation and sensitivity to cationic secretagogues. The gene expression profile obtained by the microarray analyses was found to reflect many aspects of the differentiation. Our system is thus helpful to gain deeper insights into terminal differentiation of CTMCs.

AB - To understand physiological roles of tissue mast cells, we established a culture system where bone marrow-derived immature mast cells differentiate into the connective tissue-type mast cell (CTMC)-like cells through modifying the previous co-culture system with Swiss 3T3 fibroblasts. Our system was found to reproducibly mimic the differentiation of CTMCs on the basis of several criteria, such as granule maturation and sensitivity to cationic secretagogues. The gene expression profile obtained by the microarray analyses was found to reflect many aspects of the differentiation. Our system is thus helpful to gain deeper insights into terminal differentiation of CTMCs.

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JO - FEBS Letters

JF - FEBS Letters

IS - 10

ER -

Establishment of the culture model system that reflects the process of terminal differentiation of connective tissue-type mast cells

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