Establishment of Three Types of Immortalized Human Skin Stem Cell Lines Derived from the Single Donor

Yu Inoue, Seiji Hasegawa, Yuichi Hasebe, Mika Kawagishi-Hotta, Ryosuke Okuno, Takaaki Yamada, Hiroaki Adachi, Katsuma Miyachi, Yoshie Ishii, Kazumitsu Sugiura, Hirohiko Akamatsu

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)

Abstract

Currently, human-skin derived cell culture is a basic technique essential for dermatological research, cellular engineering research, drug development, and cosmetic development. But the number of donors is limited, and primary cell function reduces through cell passage. In particular, since adult stem cells are present in a small amount in living tissues, it has been difficult to obtain a large amount of stem cells and to stably culture them. In this study, skin derived cells were isolated from the epidermis, dermis, and adipose tissue collected from single donor, and immortalization was induced through gene transfer. Subsequently, cell lines that could be used as stem cell models were selected using the differentiation potential and the expression of stem cell markers as indices, and it was confirmed that these could be stably cultured. The immortalized cell lines established in this study have the potential to be applied not only to basic dermatological research but also to a wide range of fields such as drug screening and cell engineering.

Original languageEnglish
Pages (from-to)1403-1412
Number of pages10
JournalBiological and Pharmaceutical Bulletin
Volume44
Issue number10
DOIs
Publication statusPublished - 10-2021

All Science Journal Classification (ASJC) codes

  • Pharmacology
  • Pharmaceutical Science

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