TY - JOUR
T1 - Establishment of thymoma-prone congenic rat strain, ACI.BUF/Mna-Tsr1/Tsr1
AU - Matsuyama, Mutsushi
AU - Kato, Kazuo
AU - Higo-Moriguchi, Kyoko
AU - Yamada, Tetsuji
AU - Kuramoto, Takashi
AU - Kuroda, Makoto
N1 - Funding Information:
Acknowledgments We thank Prof. T. Serikawa for his advice, and Miss K. Ishikawa and Miss J. Adachi for their technical assistance. This work was supported by the Imanaga Medical Foundation. We are also thankful to the National Bioresource-Rat for keeping rats of the congenic strain.
PY - 2008/7
Y1 - 2008/7
N2 - Purpose: To confirm the presence of the susceptible gene for the thymoma development in the region that was assumed by the previous linkage study by Oyabu et al. (J Natl Cancer Inst 91:279-282, 1999), we tried to establish a congenic strain of rats. Methods: Backcrossings between the BUF/Mna strain as a donor strain and the ACI/NMna strain as an inbred partner were repeated for 12 generations, examining whether rats had the thymoma development region, and then homozygous rats were yielded by mating among the heterozygotes. To detect the phenotypic expression, heterozygous ACI.BUF/Mna-Tsr1/+ (ACI-Tsr1/+) rats were generated by crossing female ACI.BUF/Mna-Tsr1/Tsr1 (ACI-Tsr1/Tsr1) rats with male ACI/NMna rats and were maintained for 24 months. Results: These ACI-Tsr1/+ rats produced thymoma in 71%, showing a dominant trait. The thymomas were of the lymphocyte predominant type, as those developed in rats of the original BUF/Mna strain. Conclusions: Thus, a new rat congenic strain, ACI-Tsr1/Tsr1, was established, revealing that thymoma develops in the dominant trait in ACI-Tsr1/+ rats.
AB - Purpose: To confirm the presence of the susceptible gene for the thymoma development in the region that was assumed by the previous linkage study by Oyabu et al. (J Natl Cancer Inst 91:279-282, 1999), we tried to establish a congenic strain of rats. Methods: Backcrossings between the BUF/Mna strain as a donor strain and the ACI/NMna strain as an inbred partner were repeated for 12 generations, examining whether rats had the thymoma development region, and then homozygous rats were yielded by mating among the heterozygotes. To detect the phenotypic expression, heterozygous ACI.BUF/Mna-Tsr1/+ (ACI-Tsr1/+) rats were generated by crossing female ACI.BUF/Mna-Tsr1/Tsr1 (ACI-Tsr1/Tsr1) rats with male ACI/NMna rats and were maintained for 24 months. Results: These ACI-Tsr1/+ rats produced thymoma in 71%, showing a dominant trait. The thymomas were of the lymphocyte predominant type, as those developed in rats of the original BUF/Mna strain. Conclusions: Thus, a new rat congenic strain, ACI-Tsr1/Tsr1, was established, revealing that thymoma develops in the dominant trait in ACI-Tsr1/+ rats.
KW - ACI.BUF/Mna-Trs1/Tsr1
KW - Rat model
KW - Thymoma
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U2 - 10.1007/s00432-007-0346-2
DO - 10.1007/s00432-007-0346-2
M3 - Article
C2 - 18210152
AN - SCOPUS:43449130905
SN - 0171-5216
VL - 134
SP - 789
EP - 792
JO - Journal of Cancer Research and Clinical Oncology
JF - Journal of Cancer Research and Clinical Oncology
IS - 7
ER -
