TY - JOUR
T1 - Etiological role of human papillomavirus infection in the development of penile cancer
AU - Sakamoto, Jiro
AU - Shigehara, Kazuyoshi
AU - Nakashima, Kazufumi
AU - Kawaguchi, Shohei
AU - Nakashima, Takao
AU - Shimamura, Masayoshi
AU - Yasuda, Mitsuru
AU - Kato, Taku
AU - Hasegawa, Toru
AU - Kobori, Yoshitomo
AU - Okada, Hiroshi
AU - Deguchi, Takashi
AU - Izumi, Kouji
AU - Kadono, Yoshifumi
AU - Mizokami, Atsushi
N1 - Publisher Copyright:
© 2018 The Authors
PY - 2019/1
Y1 - 2019/1
N2 - Objective: To examine the association between human papillomavirus (HPV) infection and penile cancer among Japanese patients. Methods: Thirty-four patients with penile cancer were enrolled in this study. DNA was extracted from paraffin-embedded tumor tissue samples, and HPV-DNA tests and genotyping were performed. For all of the samples, in situ hybridization (ISH) was performed to locate HPV-DNA in tumor tissue. Furthermore, expression levels of p16-INK4a, mini-chromosome maintenance protein 7 (mcm-7), HPV-L1, and Ki-67 were analyzed using immunohistochemical methods. Results: HPV and high-risk (HR)-HPV were detected in 14 (41.1%; 95% confidence interval (CI) 24.6–57.7%) and 12 (35.2%; 95% CI 19.2–51.4%) cases, respectively. HPV16 was the most frequently detected HPV type. Among the HR-HPV-positive cases, a punctate HR-HPV-DNA signal pattern was detected by ISH in tumor cell nuclei. P16-INK4a was expressed in 66.7% (95% CI 42.8–90.1%) of HR-HPV-positive cases and was significantly more frequent and stronger in HR-HPV-positive cases than in HPV-negative cases. There was no significant difference in the occurrence or distribution of mcm-7 or Ki-67 expression between HPV-positive and HPV-negative cases. HPV-L1 expression was not observed in any of the cases examined. Conclusions: HPV infection may have had an etiological role in 41% of the examined cases of penile cancer in Japan.
AB - Objective: To examine the association between human papillomavirus (HPV) infection and penile cancer among Japanese patients. Methods: Thirty-four patients with penile cancer were enrolled in this study. DNA was extracted from paraffin-embedded tumor tissue samples, and HPV-DNA tests and genotyping were performed. For all of the samples, in situ hybridization (ISH) was performed to locate HPV-DNA in tumor tissue. Furthermore, expression levels of p16-INK4a, mini-chromosome maintenance protein 7 (mcm-7), HPV-L1, and Ki-67 were analyzed using immunohistochemical methods. Results: HPV and high-risk (HR)-HPV were detected in 14 (41.1%; 95% confidence interval (CI) 24.6–57.7%) and 12 (35.2%; 95% CI 19.2–51.4%) cases, respectively. HPV16 was the most frequently detected HPV type. Among the HR-HPV-positive cases, a punctate HR-HPV-DNA signal pattern was detected by ISH in tumor cell nuclei. P16-INK4a was expressed in 66.7% (95% CI 42.8–90.1%) of HR-HPV-positive cases and was significantly more frequent and stronger in HR-HPV-positive cases than in HPV-negative cases. There was no significant difference in the occurrence or distribution of mcm-7 or Ki-67 expression between HPV-positive and HPV-negative cases. HPV-L1 expression was not observed in any of the cases examined. Conclusions: HPV infection may have had an etiological role in 41% of the examined cases of penile cancer in Japan.
KW - Human papillomavirus
KW - Ki-67
KW - Mini-chromosome maintenance protein 7
KW - Penile cancer
KW - p16-INK4a
UR - https://www.scopus.com/pages/publications/85057813985
UR - https://www.scopus.com/pages/publications/85057813985#tab=citedBy
U2 - 10.1016/j.ijid.2018.11.003
DO - 10.1016/j.ijid.2018.11.003
M3 - Article
C2 - 30423461
AN - SCOPUS:85057813985
SN - 1201-9712
VL - 78
SP - 148
EP - 154
JO - International Journal of Infectious Diseases
JF - International Journal of Infectious Diseases
ER -
