ETV6-LPXN fusion transcript generated by t(11;12)(q12.1;p13) in a patient with relapsing acute myeloid leukemia with NUP98-HOXA9

Akihiro Abe, Yukiya Yamamoto, Sachiko Iba, Tadaharu Kanie, Akinao Okamoto, Masutaka Tokuda, Yoko Inaguma, Masamitsu Yanada, Satoko Morishima, Shuichi Mizuta, Yoshiki Akatsuka, Masataka Okamoto, Toshiki Kameyama, Akila Mayeda, Nobuhiko Emi

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

ETV6, which encodes an ETS family transcription factor, is frequently rearranged in human leukemias. We show here that a patient with acute myeloid leukemia with t(7;11)(p15;p15) gained, at the time of relapse, t(11;12)(q12.1;p13) with a split ETV6 FISH signal. Using 3′-RACE PCR analysis, we found that ETV6 was fused to LPXN at 11q12.1, which encodes leupaxin. ETV6-LPXN, an in-frame fusion between exon 4 of ETV6 and exon 2 of LPXN, did not transform the interleukin-3-dependent 32D myeloid cell line to cytokine independence; however, an enhanced proliferative response was observed when these cells were treated with G-CSF without inhibition of granulocytic differentiation. The 32D and human leukemia cell lines each transduced with ETV6-LPXN showed enhanced migration towards the chemokine CXCL12. We show here for the first time that LPXN is a fusion partner of ETV6 and present evidence indicating that ETV6-LPXN plays a crucial role in leukemia progression through enhancing the response to G-CSF and CXCL12.

Original languageEnglish
Pages (from-to)242-250
Number of pages9
JournalGenes Chromosomes and Cancer
Volume55
Issue number3
DOIs
Publication statusPublished - 01-03-2016

All Science Journal Classification (ASJC) codes

  • Genetics
  • Cancer Research

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