Evaluation of asialoglycoprotein receptor imaging agent as a marker of hepatic ischemia-reperfusion injury and recovery

Hiroshi Toyama, Kazuo Suzuki, Aiko Naito, Makoto Kuroda, Kaoru Kikukawa, Yoshiyuki Komori, Akitake Hasumi, Kaname Matsumura, Toshiteru Fujiwara, Kiyonobu Ito, Kazutaka Ejiri, Kohei Senda, Akira Takeuchi, Sukehiko Koga

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Protection of hepatocytes from ischemia-reperfusion injury is a clinically important issue. The purpose of this study was to evaluate changes in acute liver damage and recovery after ischemia-reperfusion in rats with asialoglycoprotein receptor (ASGP-R) ligand. Ischemia was induced by clamping the hepatoduodenal ligament for 90 min. At 1, 3, 24, 48 hr, 1 and 2 wk after reperfusion, I-125-GSA was injected. Five min after injection, blood samples were obtained and the liver was removed. Several regions from each lobe were dissected, weighed and counted. Mean uptakes (% dose/g) in the liver and blood samples were calculated. Histologic sections stained with hematoxylin- eosin (H-E) stain showed ischemic damage at 1 and 3 hr, and focal hepatocyte necrosis at 24 hr. Predominant massive necrosis was not seen. The mitotic index with H-E stain and proliferating cell nuclear antigen (PCNA) labeling index were highest at 1 wk, indicating liver regeneration. At 1 and 3 hr, liver uptake was significantly decreased, and blood uptake was significantly increased, indicating decreased tissue blood flow and ischemic damage. Liver uptake showed significant increases at 48 hr and 1 wk, and was the highest at 1 wk, indicating liver regeneration during the convalescence stage. ASGP-R binding may provide valuable information on ischemia-reperfusion injury and recovery.

Original languageEnglish
Pages (from-to)155-160
Number of pages6
JournalAnnals of Nuclear Medicine
Volume13
Issue number3
DOIs
Publication statusPublished - 01-01-1999

Fingerprint

Asialoglycoprotein Receptor
Reperfusion Injury
Liver
Liver Regeneration
Hematoxylin
Eosine Yellowish-(YS)
Reperfusion
Hepatocytes
Necrosis
Coloring Agents
Ischemia
Mitotic Index
Proliferating Cell Nuclear Antigen
Ligaments
Constriction
Ligands
Injections

All Science Journal Classification (ASJC) codes

  • Radiology Nuclear Medicine and imaging

Cite this

Toyama, Hiroshi ; Suzuki, Kazuo ; Naito, Aiko ; Kuroda, Makoto ; Kikukawa, Kaoru ; Komori, Yoshiyuki ; Hasumi, Akitake ; Matsumura, Kaname ; Fujiwara, Toshiteru ; Ito, Kiyonobu ; Ejiri, Kazutaka ; Senda, Kohei ; Takeuchi, Akira ; Koga, Sukehiko. / Evaluation of asialoglycoprotein receptor imaging agent as a marker of hepatic ischemia-reperfusion injury and recovery. In: Annals of Nuclear Medicine. 1999 ; Vol. 13, No. 3. pp. 155-160.
@article{b33c3da7e87e46f8a6e3c265466303fd,
title = "Evaluation of asialoglycoprotein receptor imaging agent as a marker of hepatic ischemia-reperfusion injury and recovery",
abstract = "Protection of hepatocytes from ischemia-reperfusion injury is a clinically important issue. The purpose of this study was to evaluate changes in acute liver damage and recovery after ischemia-reperfusion in rats with asialoglycoprotein receptor (ASGP-R) ligand. Ischemia was induced by clamping the hepatoduodenal ligament for 90 min. At 1, 3, 24, 48 hr, 1 and 2 wk after reperfusion, I-125-GSA was injected. Five min after injection, blood samples were obtained and the liver was removed. Several regions from each lobe were dissected, weighed and counted. Mean uptakes ({\%} dose/g) in the liver and blood samples were calculated. Histologic sections stained with hematoxylin- eosin (H-E) stain showed ischemic damage at 1 and 3 hr, and focal hepatocyte necrosis at 24 hr. Predominant massive necrosis was not seen. The mitotic index with H-E stain and proliferating cell nuclear antigen (PCNA) labeling index were highest at 1 wk, indicating liver regeneration. At 1 and 3 hr, liver uptake was significantly decreased, and blood uptake was significantly increased, indicating decreased tissue blood flow and ischemic damage. Liver uptake showed significant increases at 48 hr and 1 wk, and was the highest at 1 wk, indicating liver regeneration during the convalescence stage. ASGP-R binding may provide valuable information on ischemia-reperfusion injury and recovery.",
author = "Hiroshi Toyama and Kazuo Suzuki and Aiko Naito and Makoto Kuroda and Kaoru Kikukawa and Yoshiyuki Komori and Akitake Hasumi and Kaname Matsumura and Toshiteru Fujiwara and Kiyonobu Ito and Kazutaka Ejiri and Kohei Senda and Akira Takeuchi and Sukehiko Koga",
year = "1999",
month = "1",
day = "1",
doi = "10.1007/BF03164855",
language = "English",
volume = "13",
pages = "155--160",
journal = "Annals of Nuclear Medicine",
issn = "0914-7187",
publisher = "Springer Japan",
number = "3",

}

Toyama, H, Suzuki, K, Naito, A, Kuroda, M, Kikukawa, K, Komori, Y, Hasumi, A, Matsumura, K, Fujiwara, T, Ito, K, Ejiri, K, Senda, K, Takeuchi, A & Koga, S 1999, 'Evaluation of asialoglycoprotein receptor imaging agent as a marker of hepatic ischemia-reperfusion injury and recovery', Annals of Nuclear Medicine, vol. 13, no. 3, pp. 155-160. https://doi.org/10.1007/BF03164855

Evaluation of asialoglycoprotein receptor imaging agent as a marker of hepatic ischemia-reperfusion injury and recovery. / Toyama, Hiroshi; Suzuki, Kazuo; Naito, Aiko; Kuroda, Makoto; Kikukawa, Kaoru; Komori, Yoshiyuki; Hasumi, Akitake; Matsumura, Kaname; Fujiwara, Toshiteru; Ito, Kiyonobu; Ejiri, Kazutaka; Senda, Kohei; Takeuchi, Akira; Koga, Sukehiko.

In: Annals of Nuclear Medicine, Vol. 13, No. 3, 01.01.1999, p. 155-160.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Evaluation of asialoglycoprotein receptor imaging agent as a marker of hepatic ischemia-reperfusion injury and recovery

AU - Toyama, Hiroshi

AU - Suzuki, Kazuo

AU - Naito, Aiko

AU - Kuroda, Makoto

AU - Kikukawa, Kaoru

AU - Komori, Yoshiyuki

AU - Hasumi, Akitake

AU - Matsumura, Kaname

AU - Fujiwara, Toshiteru

AU - Ito, Kiyonobu

AU - Ejiri, Kazutaka

AU - Senda, Kohei

AU - Takeuchi, Akira

AU - Koga, Sukehiko

PY - 1999/1/1

Y1 - 1999/1/1

N2 - Protection of hepatocytes from ischemia-reperfusion injury is a clinically important issue. The purpose of this study was to evaluate changes in acute liver damage and recovery after ischemia-reperfusion in rats with asialoglycoprotein receptor (ASGP-R) ligand. Ischemia was induced by clamping the hepatoduodenal ligament for 90 min. At 1, 3, 24, 48 hr, 1 and 2 wk after reperfusion, I-125-GSA was injected. Five min after injection, blood samples were obtained and the liver was removed. Several regions from each lobe were dissected, weighed and counted. Mean uptakes (% dose/g) in the liver and blood samples were calculated. Histologic sections stained with hematoxylin- eosin (H-E) stain showed ischemic damage at 1 and 3 hr, and focal hepatocyte necrosis at 24 hr. Predominant massive necrosis was not seen. The mitotic index with H-E stain and proliferating cell nuclear antigen (PCNA) labeling index were highest at 1 wk, indicating liver regeneration. At 1 and 3 hr, liver uptake was significantly decreased, and blood uptake was significantly increased, indicating decreased tissue blood flow and ischemic damage. Liver uptake showed significant increases at 48 hr and 1 wk, and was the highest at 1 wk, indicating liver regeneration during the convalescence stage. ASGP-R binding may provide valuable information on ischemia-reperfusion injury and recovery.

AB - Protection of hepatocytes from ischemia-reperfusion injury is a clinically important issue. The purpose of this study was to evaluate changes in acute liver damage and recovery after ischemia-reperfusion in rats with asialoglycoprotein receptor (ASGP-R) ligand. Ischemia was induced by clamping the hepatoduodenal ligament for 90 min. At 1, 3, 24, 48 hr, 1 and 2 wk after reperfusion, I-125-GSA was injected. Five min after injection, blood samples were obtained and the liver was removed. Several regions from each lobe were dissected, weighed and counted. Mean uptakes (% dose/g) in the liver and blood samples were calculated. Histologic sections stained with hematoxylin- eosin (H-E) stain showed ischemic damage at 1 and 3 hr, and focal hepatocyte necrosis at 24 hr. Predominant massive necrosis was not seen. The mitotic index with H-E stain and proliferating cell nuclear antigen (PCNA) labeling index were highest at 1 wk, indicating liver regeneration. At 1 and 3 hr, liver uptake was significantly decreased, and blood uptake was significantly increased, indicating decreased tissue blood flow and ischemic damage. Liver uptake showed significant increases at 48 hr and 1 wk, and was the highest at 1 wk, indicating liver regeneration during the convalescence stage. ASGP-R binding may provide valuable information on ischemia-reperfusion injury and recovery.

UR - http://www.scopus.com/inward/record.url?scp=0032802696&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0032802696&partnerID=8YFLogxK

U2 - 10.1007/BF03164855

DO - 10.1007/BF03164855

M3 - Article

C2 - 10435375

AN - SCOPUS:0032802696

VL - 13

SP - 155

EP - 160

JO - Annals of Nuclear Medicine

JF - Annals of Nuclear Medicine

SN - 0914-7187

IS - 3

ER -