Evaluation of asialoglycoprotein receptor imaging agent as a marker of hepatic ischemia-reperfusion injury and recovery

Hiroshi Toyama, Kazuo Suzuki, Aiko Naito, Makoto Kuroda, Kaoru Kikukawa, Yoshiyuki Komori, Akitake Hasumi, Kaname Matsumura, Toshiteru Fujiwara, Kiyonobu Ito, Kazutaka Ejiri, Kohei Senda, Akira Takeuchi, Sukehiko Koga

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4 Citations (Scopus)


Protection of hepatocytes from ischemia-reperfusion injury is a clinically important issue. The purpose of this study was to evaluate changes in acute liver damage and recovery after ischemia-reperfusion in rats with asialoglycoprotein receptor (ASGP-R) ligand. Ischemia was induced by clamping the hepatoduodenal ligament for 90 min. At 1, 3, 24, 48 hr, 1 and 2 wk after reperfusion, I-125-GSA was injected. Five min after injection, blood samples were obtained and the liver was removed. Several regions from each lobe were dissected, weighed and counted. Mean uptakes (% dose/g) in the liver and blood samples were calculated. Histologic sections stained with hematoxylin- eosin (H-E) stain showed ischemic damage at 1 and 3 hr, and focal hepatocyte necrosis at 24 hr. Predominant massive necrosis was not seen. The mitotic index with H-E stain and proliferating cell nuclear antigen (PCNA) labeling index were highest at 1 wk, indicating liver regeneration. At 1 and 3 hr, liver uptake was significantly decreased, and blood uptake was significantly increased, indicating decreased tissue blood flow and ischemic damage. Liver uptake showed significant increases at 48 hr and 1 wk, and was the highest at 1 wk, indicating liver regeneration during the convalescence stage. ASGP-R binding may provide valuable information on ischemia-reperfusion injury and recovery.

Original languageEnglish
Pages (from-to)155-160
Number of pages6
JournalAnnals of Nuclear Medicine
Issue number3
Publication statusPublished - 1999

All Science Journal Classification (ASJC) codes

  • Radiology Nuclear Medicine and imaging


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