Evaluation of endocrine disrupting activity of plasticizers in polyvinyl chloride tubes by estrogen receptor alpha binding assay

Atsushi Ohashi, Hirohisa Kotera, Hideo Hori, Makoto Hibiya, Koji Watanabe, Kazutaka Murakami, Midori Hasegawa, Makoto Tomita, Yoshinobu Hiki, Satoshi Sugiyama

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

Polyvinyl chloride (PVC) tubing is an indispensable medical material for extracorporeal circulation therapy. However, di(2-ethylhexyl)phthalate (DEHP), a suspected endocrine disruptor, can be eluted from PVC, suggesting that an alternative material that does not contain DEHP is needed for clinical applications. First, we evaluated the endocrine disrupting risks of the plasticizers contained in PVC tubes by investigating their binding affinities for the human estrogen receptor alpha (ERα). Our results revealed that, while DEHP has some binding affinity for ERα, neither epoxidized soybean oil nor tris(2-ethylhexyl)trimellitate (an alternative to DEHP) has any affinity for ERα. Second, we evaluated the endocrine disrupting risks of a tube made of newly developed plasticizer-free (PF) materials. We confirmed the presence of DEHP and detected several unidentified substances in plasma stored within the PVC tube. This plasma's competitive binding affinity for ERα was significantly higher than that of control plasma (P < 0.01). In contrast, the profile of plasma stored in the PF tube was similar to that of the control, both in terms of high-performance liquid chromatography chromatograms and competitive binding capacity for ERα, suggesting that the PF tube is biocompatible and is useful for reducing the elution of substances capable of binding to ERα.

Original languageEnglish
Pages (from-to)252-256
Number of pages5
JournalJournal of Artificial Organs
Volume8
Issue number4
DOIs
Publication statusPublished - 01-12-2005

Fingerprint

Plasticizers
Estrogen Receptor alpha
Polyvinyl Chloride
Polyvinyl chlorides
Assays
Plasmas
Competitive Binding
Endocrine Disruptors
Extracorporeal Circulation
High performance liquid chromatography
Tubing
Soybean oil
High Pressure Liquid Chromatography
phthalic acid
Estrogens

All Science Journal Classification (ASJC) codes

  • Medicine (miscellaneous)
  • Biomaterials
  • Biomedical Engineering
  • Cardiology and Cardiovascular Medicine

Cite this

Ohashi, Atsushi ; Kotera, Hirohisa ; Hori, Hideo ; Hibiya, Makoto ; Watanabe, Koji ; Murakami, Kazutaka ; Hasegawa, Midori ; Tomita, Makoto ; Hiki, Yoshinobu ; Sugiyama, Satoshi. / Evaluation of endocrine disrupting activity of plasticizers in polyvinyl chloride tubes by estrogen receptor alpha binding assay. In: Journal of Artificial Organs. 2005 ; Vol. 8, No. 4. pp. 252-256.
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Evaluation of endocrine disrupting activity of plasticizers in polyvinyl chloride tubes by estrogen receptor alpha binding assay. / Ohashi, Atsushi; Kotera, Hirohisa; Hori, Hideo; Hibiya, Makoto; Watanabe, Koji; Murakami, Kazutaka; Hasegawa, Midori; Tomita, Makoto; Hiki, Yoshinobu; Sugiyama, Satoshi.

In: Journal of Artificial Organs, Vol. 8, No. 4, 01.12.2005, p. 252-256.

Research output: Contribution to journalArticle

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T1 - Evaluation of endocrine disrupting activity of plasticizers in polyvinyl chloride tubes by estrogen receptor alpha binding assay

AU - Ohashi, Atsushi

AU - Kotera, Hirohisa

AU - Hori, Hideo

AU - Hibiya, Makoto

AU - Watanabe, Koji

AU - Murakami, Kazutaka

AU - Hasegawa, Midori

AU - Tomita, Makoto

AU - Hiki, Yoshinobu

AU - Sugiyama, Satoshi

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