TY - JOUR
T1 - Evaluation of intestinal microbiota, short-chain fatty acids, and immunoglobulin a in diversion colitis
AU - Tominaga, Kentaro
AU - Tsuchiya, Atsunori
AU - Mizusawa, Takeshi
AU - Matsumoto, Asami
AU - Minemura, Ayaka
AU - Oka, Kentaro
AU - Takahashi, Motomichi
AU - Yosida, Tomoaki
AU - Kawata, Yuzo
AU - Takahashi, Kazuya
AU - Sato, Hiroki
AU - Ikarashi, Satoshi
AU - Hayashi, Kazunao
AU - Mizuno, Ken ichi
AU - Tajima, Yosuke
AU - Nakano, Masato
AU - Shimada, Yoshifumi
AU - Kameyama, Hitoshi
AU - Yokoyama, Junji
AU - Wakai, Toshifumi
AU - Terai, Shuji
N1 - Funding Information:
These authors disclose the following: Shuji Terai received research funding from Miyarisan Pharmaceutical Co., Ltd. The remaining authors have no conflicts of interest to disclose.This work was supported by Takeda Japan Medical Office Funded Research Grant 2018, Tsukada Medical Funded Research Grant, Grant-in-Aid for Young Scientific Research (19K17393) from the Ministry of Education, Science, Technology, Sports, and Miyarisan Pharmaceutical Co., Ltd.The authors disclose the following: Shuji Terai received research funding from Miyarisan Pharmaceutical Co., Ltd. Asami Matsumoto, Ayaka Minemura, Kentaro Oka and Motomichi Takahashi are employees of Miyarisan Pharmaceutical Co., Ltd.The remaining authors disclose no conflicts.
Publisher Copyright:
© 2020 The Authors
PY - 2021/3
Y1 - 2021/3
N2 - It is reported that an increase in aerobic bacteria, a lack of short-chain fatty acids (SCFAs), and immune disorders in the diverted colon are major causes of diversion colitis. However, the precise pathogenesis of this condition remains unclear. The aim of the present study was to examine the microbiota, intestinal SCFAs, and immunoglobulin A (IgA) in the diverted colon. Eight patients underwent operative procedures for colostomies. We assessed the diverted colon using endoscopy and obtained intestinal samples from the diverted colon and oral colon in these patients. We analyzed the microbiota and SCFAs of the intestinal samples. The bacterial communities were investigated using a 16S rRNA gene sequencing method. The microbiota demonstrated a change in the proportion of some species, especially Lactobacillus, which significantly decreased in the diverted colon at the genus level. We also showed that intestinal SCFA values were significantly decreased in the diverted colon. Furthermore, intestinal IgA levels were significantly increased in the diverted colon. This study was the first to show that intestinal SCFAs were significantly decreased and intestinal IgA was significantly increased in the diverted colon. Our data suggest that SCFAs affect the microbiota and may play an immunological role in diversion colitis.
AB - It is reported that an increase in aerobic bacteria, a lack of short-chain fatty acids (SCFAs), and immune disorders in the diverted colon are major causes of diversion colitis. However, the precise pathogenesis of this condition remains unclear. The aim of the present study was to examine the microbiota, intestinal SCFAs, and immunoglobulin A (IgA) in the diverted colon. Eight patients underwent operative procedures for colostomies. We assessed the diverted colon using endoscopy and obtained intestinal samples from the diverted colon and oral colon in these patients. We analyzed the microbiota and SCFAs of the intestinal samples. The bacterial communities were investigated using a 16S rRNA gene sequencing method. The microbiota demonstrated a change in the proportion of some species, especially Lactobacillus, which significantly decreased in the diverted colon at the genus level. We also showed that intestinal SCFA values were significantly decreased in the diverted colon. Furthermore, intestinal IgA levels were significantly increased in the diverted colon. This study was the first to show that intestinal SCFAs were significantly decreased and intestinal IgA was significantly increased in the diverted colon. Our data suggest that SCFAs affect the microbiota and may play an immunological role in diversion colitis.
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U2 - 10.1016/j.bbrep.2020.100892
DO - 10.1016/j.bbrep.2020.100892
M3 - Article
AN - SCOPUS:85098543816
VL - 25
JO - Biochemistry and Biophysics Reports
JF - Biochemistry and Biophysics Reports
SN - 2405-5808
M1 - 100892
ER -