Evaluation of retinal function in streptozotocin-induced diabetic rats by using the electroretinography and immunohistochemistry methods

Noriko Hiramatsu, Saori Deguchi, Chiaki Yoshioka, Hiroko Otake, Naoki Yamamoto, Noriaki Nagai

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Streptozotocin-induced diabetic rat (STZ rat) was used in many studies for the diabetic mellitus. In this study, we demonstrated whether the electroretinograms (ERG) was changed in the retina of STZ rats. In addition, we investigated the histopathological alteration in the retina of STZ rats by using the immunological method. The 100 mg/kg of STZ was injected continuously for 2 d (100 mg/kg×2). The insulin level was decreased, and the glucose level was enhanced 14 d after the injection of STZ. Moreover, the levels of a-wave, b-wave and OP amplitude were decreased in the rat at 14 d after the injection of STZ. Although, the damage and apoptosis was not observed in the retinal ganglion cell of STZ rats by the immunological experiment using the phospho-H2A.X and cleaved caspase-3, the distance between cell and cell was increased in both of outer- and inner- nuclear (granule) layer in retina of STZ rats. In conclusion, we showed that the enhanced thickening in retina was caused by the injection of excessive STZ. The thickening in retina of STZ rats may lead to the dysfunction of retina, resulting in the decrease in ERG. These findings provide significant information that can be used in the design of a model of diabetic retinopathy.

Original languageEnglish
Pages (from-to)1169-1175
Number of pages7
JournalYakugaku Zasshi
Volume137
Issue number9
DOIs
Publication statusPublished - 01-01-2017

Fingerprint

Electroretinography
Streptozocin
Immunohistochemistry
Retina
Injections
Retinal Ganglion Cells
Diabetic Retinopathy
Caspase 3
Insulin
Apoptosis
Glucose

All Science Journal Classification (ASJC) codes

  • Pharmacology
  • Pharmaceutical Science

Cite this

@article{27aae724953643c893aad067d1097129,
title = "Evaluation of retinal function in streptozotocin-induced diabetic rats by using the electroretinography and immunohistochemistry methods",
abstract = "Streptozotocin-induced diabetic rat (STZ rat) was used in many studies for the diabetic mellitus. In this study, we demonstrated whether the electroretinograms (ERG) was changed in the retina of STZ rats. In addition, we investigated the histopathological alteration in the retina of STZ rats by using the immunological method. The 100 mg/kg of STZ was injected continuously for 2 d (100 mg/kg×2). The insulin level was decreased, and the glucose level was enhanced 14 d after the injection of STZ. Moreover, the levels of a-wave, b-wave and OP amplitude were decreased in the rat at 14 d after the injection of STZ. Although, the damage and apoptosis was not observed in the retinal ganglion cell of STZ rats by the immunological experiment using the phospho-H2A.X and cleaved caspase-3, the distance between cell and cell was increased in both of outer- and inner- nuclear (granule) layer in retina of STZ rats. In conclusion, we showed that the enhanced thickening in retina was caused by the injection of excessive STZ. The thickening in retina of STZ rats may lead to the dysfunction of retina, resulting in the decrease in ERG. These findings provide significant information that can be used in the design of a model of diabetic retinopathy.",
author = "Noriko Hiramatsu and Saori Deguchi and Chiaki Yoshioka and Hiroko Otake and Naoki Yamamoto and Noriaki Nagai",
year = "2017",
month = "1",
day = "1",
doi = "10.1248/yakushi.17-00115",
language = "English",
volume = "137",
pages = "1169--1175",
journal = "Yakugaku Zasshi",
issn = "0031-6903",
publisher = "Pharmaceutical Society of Japan",
number = "9",

}

Evaluation of retinal function in streptozotocin-induced diabetic rats by using the electroretinography and immunohistochemistry methods. / Hiramatsu, Noriko; Deguchi, Saori; Yoshioka, Chiaki; Otake, Hiroko; Yamamoto, Naoki; Nagai, Noriaki.

In: Yakugaku Zasshi, Vol. 137, No. 9, 01.01.2017, p. 1169-1175.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Evaluation of retinal function in streptozotocin-induced diabetic rats by using the electroretinography and immunohistochemistry methods

AU - Hiramatsu, Noriko

AU - Deguchi, Saori

AU - Yoshioka, Chiaki

AU - Otake, Hiroko

AU - Yamamoto, Naoki

AU - Nagai, Noriaki

PY - 2017/1/1

Y1 - 2017/1/1

N2 - Streptozotocin-induced diabetic rat (STZ rat) was used in many studies for the diabetic mellitus. In this study, we demonstrated whether the electroretinograms (ERG) was changed in the retina of STZ rats. In addition, we investigated the histopathological alteration in the retina of STZ rats by using the immunological method. The 100 mg/kg of STZ was injected continuously for 2 d (100 mg/kg×2). The insulin level was decreased, and the glucose level was enhanced 14 d after the injection of STZ. Moreover, the levels of a-wave, b-wave and OP amplitude were decreased in the rat at 14 d after the injection of STZ. Although, the damage and apoptosis was not observed in the retinal ganglion cell of STZ rats by the immunological experiment using the phospho-H2A.X and cleaved caspase-3, the distance between cell and cell was increased in both of outer- and inner- nuclear (granule) layer in retina of STZ rats. In conclusion, we showed that the enhanced thickening in retina was caused by the injection of excessive STZ. The thickening in retina of STZ rats may lead to the dysfunction of retina, resulting in the decrease in ERG. These findings provide significant information that can be used in the design of a model of diabetic retinopathy.

AB - Streptozotocin-induced diabetic rat (STZ rat) was used in many studies for the diabetic mellitus. In this study, we demonstrated whether the electroretinograms (ERG) was changed in the retina of STZ rats. In addition, we investigated the histopathological alteration in the retina of STZ rats by using the immunological method. The 100 mg/kg of STZ was injected continuously for 2 d (100 mg/kg×2). The insulin level was decreased, and the glucose level was enhanced 14 d after the injection of STZ. Moreover, the levels of a-wave, b-wave and OP amplitude were decreased in the rat at 14 d after the injection of STZ. Although, the damage and apoptosis was not observed in the retinal ganglion cell of STZ rats by the immunological experiment using the phospho-H2A.X and cleaved caspase-3, the distance between cell and cell was increased in both of outer- and inner- nuclear (granule) layer in retina of STZ rats. In conclusion, we showed that the enhanced thickening in retina was caused by the injection of excessive STZ. The thickening in retina of STZ rats may lead to the dysfunction of retina, resulting in the decrease in ERG. These findings provide significant information that can be used in the design of a model of diabetic retinopathy.

UR - http://www.scopus.com/inward/record.url?scp=85028635109&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85028635109&partnerID=8YFLogxK

U2 - 10.1248/yakushi.17-00115

DO - 10.1248/yakushi.17-00115

M3 - Article

VL - 137

SP - 1169

EP - 1175

JO - Yakugaku Zasshi

JF - Yakugaku Zasshi

SN - 0031-6903

IS - 9

ER -