Evaluation of reverse transcription loop- mediated isothermal amplification assays for rapid diagnosis of pandemic influenza A/H1N1 2009 virus

Mina Nakauchi, Tetsushi Yoshikawa, Hidetaka Nakai, Ken Sugata, Akiko Yoshikawa, Yoshizo Asano, Masaru Ihira, Masato Tashiro, Tsutomu Kageyama

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Two genetic diagnosis systems using reverse transcription- loop- mediated isothermal amplification (RT- LAMP) technology were evaluated: one for detecting the HA gene of the pandemic influenza A/H1N1 2009 virus (H1pdm RT- LAMP) and the other for detecting the matrix gene of the influenza A virus (TypeA RT- LAMP). The competence of these two RT- LAMP assay kits for the diagnosis of the pandemic influenza A/H1N1 2009 virus was compared using real- time RT- PCR assays developed recently on viruses isolated and clinical specimens collected from patients with suspected infection. TypeA RT- LAMP and H1pdm RT- LAMP showed almost the same sensitivity as real- time RT- PCR for viruses isolated. The sensitivity and specificity of TypeA RT- LAMP and H1pdm RT- LAMP were 96.3% and 88.9%, respectively, for clinical specimens. Considering that the ability of the two RT- LAMP assay kits for detection of the pandemic influenza A/H1N1 2009 virus was comparable to that of the real- time RT- PCR assays, and that the assays were completed within 1 hr and did not require any expensive equipment, these two RT- LAMP assays are promising rapid diagnostic tests for the pandemic influenza A/H1N1 2009 virus at the hospital bedside.

Original languageEnglish
Pages (from-to)10-15
Number of pages6
JournalJournal of Medical Virology
Volume83
Issue number1
DOIs
Publication statusPublished - 01-01-2011

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H1N1 Subtype Influenza A Virus
Pandemics
Human Influenza
Reverse Transcription
Real-Time Polymerase Chain Reaction
Viruses
Aptitude
Influenza A virus
Routine Diagnostic Tests
Mental Competency
Genes
Technology
Sensitivity and Specificity

All Science Journal Classification (ASJC) codes

  • Virology
  • Infectious Diseases

Cite this

Nakauchi, Mina ; Yoshikawa, Tetsushi ; Nakai, Hidetaka ; Sugata, Ken ; Yoshikawa, Akiko ; Asano, Yoshizo ; Ihira, Masaru ; Tashiro, Masato ; Kageyama, Tsutomu. / Evaluation of reverse transcription loop- mediated isothermal amplification assays for rapid diagnosis of pandemic influenza A/H1N1 2009 virus. In: Journal of Medical Virology. 2011 ; Vol. 83, No. 1. pp. 10-15.
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Evaluation of reverse transcription loop- mediated isothermal amplification assays for rapid diagnosis of pandemic influenza A/H1N1 2009 virus. / Nakauchi, Mina; Yoshikawa, Tetsushi; Nakai, Hidetaka; Sugata, Ken; Yoshikawa, Akiko; Asano, Yoshizo; Ihira, Masaru; Tashiro, Masato; Kageyama, Tsutomu.

In: Journal of Medical Virology, Vol. 83, No. 1, 01.01.2011, p. 10-15.

Research output: Contribution to journalArticle

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