Evaluation of the cross-reactivity of antigens in Glupearl 19S and other hydrolysed wheat proteins in cosmetics

Masashi Nakamura, Akiko Yagami, Kazuhiro Hara, Akiyo Sano-Nagai, Tsukane Kobayashi, Kayoko Matsunaga

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Background: In Japan, over 2000 users of a facial soap containing Glupearl 19S (GP19S), a hydrolysed wheat protein (HWP), developed immediate-type systemic wheat allergy (HWP-IWA), and ∼70% of them developed associated contact urticaria. Objectives: We investigated whether HWP-IWA patients cross-react with other HWPs, and analysed HWP antigenic characteristics. Methods: We used 10 types of HWP that are commercially available as cosmetic ingredients, and 16 subjects with HWP-IWA. We performed an enzyme-linked immunosorbent assay (ELISA) to evaluate the reactivity to each HWP, and western blotting to evaluate the characteristics of the antigens by using HWP-IWA patients' serum IgE antibodies. We also performed prick tests with the HWPs. Results: The patients reacted to four other HWPs in addition to GP19S, according to ELISA, and this was confirmed by strong reactions in the prick tests to the same four types of HWP. Smears of antigens with molecular weights ranging from the high range to the low range were seen on western blotting with the four HWPs that showed strong reactions in the ELISA and prick tests. Conclusions: HWP-IWA patients cross-react with other HWPs. The antigens that they cross-reacted to had a molecular weight distribution similar to that of GP19S present in the HWPs.

Original languageEnglish
Pages (from-to)346-352
Number of pages7
JournalContact Dermatitis
Volume74
Issue number6
DOIs
Publication statusPublished - 01-01-2016

Fingerprint

Cosmetics
Triticum
Antigens
Proteins
Enzyme-Linked Immunosorbent Assay
Wheat Hypersensitivity
Molecular Weight
Western Blotting
Glupearl 19S
Soaps
Urticaria
Immunoglobulin E
Japan
Antibodies
Serum

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Dermatology

Cite this

Nakamura, Masashi ; Yagami, Akiko ; Hara, Kazuhiro ; Sano-Nagai, Akiyo ; Kobayashi, Tsukane ; Matsunaga, Kayoko. / Evaluation of the cross-reactivity of antigens in Glupearl 19S and other hydrolysed wheat proteins in cosmetics. In: Contact Dermatitis. 2016 ; Vol. 74, No. 6. pp. 346-352.
@article{8d2f4eb9fa9a455eb375160b82c744ab,
title = "Evaluation of the cross-reactivity of antigens in Glupearl 19S and other hydrolysed wheat proteins in cosmetics",
abstract = "Background: In Japan, over 2000 users of a facial soap containing Glupearl 19S (GP19S), a hydrolysed wheat protein (HWP), developed immediate-type systemic wheat allergy (HWP-IWA), and ∼70{\%} of them developed associated contact urticaria. Objectives: We investigated whether HWP-IWA patients cross-react with other HWPs, and analysed HWP antigenic characteristics. Methods: We used 10 types of HWP that are commercially available as cosmetic ingredients, and 16 subjects with HWP-IWA. We performed an enzyme-linked immunosorbent assay (ELISA) to evaluate the reactivity to each HWP, and western blotting to evaluate the characteristics of the antigens by using HWP-IWA patients' serum IgE antibodies. We also performed prick tests with the HWPs. Results: The patients reacted to four other HWPs in addition to GP19S, according to ELISA, and this was confirmed by strong reactions in the prick tests to the same four types of HWP. Smears of antigens with molecular weights ranging from the high range to the low range were seen on western blotting with the four HWPs that showed strong reactions in the ELISA and prick tests. Conclusions: HWP-IWA patients cross-react with other HWPs. The antigens that they cross-reacted to had a molecular weight distribution similar to that of GP19S present in the HWPs.",
author = "Masashi Nakamura and Akiko Yagami and Kazuhiro Hara and Akiyo Sano-Nagai and Tsukane Kobayashi and Kayoko Matsunaga",
year = "2016",
month = "1",
day = "1",
doi = "10.1111/cod.12551",
language = "English",
volume = "74",
pages = "346--352",
journal = "Contact Dermatitis",
issn = "0105-1873",
publisher = "Wiley-Blackwell",
number = "6",

}

Evaluation of the cross-reactivity of antigens in Glupearl 19S and other hydrolysed wheat proteins in cosmetics. / Nakamura, Masashi; Yagami, Akiko; Hara, Kazuhiro; Sano-Nagai, Akiyo; Kobayashi, Tsukane; Matsunaga, Kayoko.

In: Contact Dermatitis, Vol. 74, No. 6, 01.01.2016, p. 346-352.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Evaluation of the cross-reactivity of antigens in Glupearl 19S and other hydrolysed wheat proteins in cosmetics

AU - Nakamura, Masashi

AU - Yagami, Akiko

AU - Hara, Kazuhiro

AU - Sano-Nagai, Akiyo

AU - Kobayashi, Tsukane

AU - Matsunaga, Kayoko

PY - 2016/1/1

Y1 - 2016/1/1

N2 - Background: In Japan, over 2000 users of a facial soap containing Glupearl 19S (GP19S), a hydrolysed wheat protein (HWP), developed immediate-type systemic wheat allergy (HWP-IWA), and ∼70% of them developed associated contact urticaria. Objectives: We investigated whether HWP-IWA patients cross-react with other HWPs, and analysed HWP antigenic characteristics. Methods: We used 10 types of HWP that are commercially available as cosmetic ingredients, and 16 subjects with HWP-IWA. We performed an enzyme-linked immunosorbent assay (ELISA) to evaluate the reactivity to each HWP, and western blotting to evaluate the characteristics of the antigens by using HWP-IWA patients' serum IgE antibodies. We also performed prick tests with the HWPs. Results: The patients reacted to four other HWPs in addition to GP19S, according to ELISA, and this was confirmed by strong reactions in the prick tests to the same four types of HWP. Smears of antigens with molecular weights ranging from the high range to the low range were seen on western blotting with the four HWPs that showed strong reactions in the ELISA and prick tests. Conclusions: HWP-IWA patients cross-react with other HWPs. The antigens that they cross-reacted to had a molecular weight distribution similar to that of GP19S present in the HWPs.

AB - Background: In Japan, over 2000 users of a facial soap containing Glupearl 19S (GP19S), a hydrolysed wheat protein (HWP), developed immediate-type systemic wheat allergy (HWP-IWA), and ∼70% of them developed associated contact urticaria. Objectives: We investigated whether HWP-IWA patients cross-react with other HWPs, and analysed HWP antigenic characteristics. Methods: We used 10 types of HWP that are commercially available as cosmetic ingredients, and 16 subjects with HWP-IWA. We performed an enzyme-linked immunosorbent assay (ELISA) to evaluate the reactivity to each HWP, and western blotting to evaluate the characteristics of the antigens by using HWP-IWA patients' serum IgE antibodies. We also performed prick tests with the HWPs. Results: The patients reacted to four other HWPs in addition to GP19S, according to ELISA, and this was confirmed by strong reactions in the prick tests to the same four types of HWP. Smears of antigens with molecular weights ranging from the high range to the low range were seen on western blotting with the four HWPs that showed strong reactions in the ELISA and prick tests. Conclusions: HWP-IWA patients cross-react with other HWPs. The antigens that they cross-reacted to had a molecular weight distribution similar to that of GP19S present in the HWPs.

UR - http://www.scopus.com/inward/record.url?scp=84962915296&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84962915296&partnerID=8YFLogxK

U2 - 10.1111/cod.12551

DO - 10.1111/cod.12551

M3 - Article

C2 - 27027256

AN - SCOPUS:84962915296

VL - 74

SP - 346

EP - 352

JO - Contact Dermatitis

JF - Contact Dermatitis

SN - 0105-1873

IS - 6

ER -