Evaluation of the Usefulness of Human Adipose-Derived Stem Cell Spheroids Formed Using SphereRing® and the Lethal Damage Sensitivity to Synovial Fluid In Vitro

Atsushi Fuku, Yasuhiko Taki, Yuka Nakamura, Hironori Kitajima, Takashi Takaki, Terutsugu Koya, Ikuhiro Tanida, Kaori Nozaki, Hiroshi Sunami, Hiroaki Hirata, Yoshiyuki Tachi, Togen Masauji, Naoki Yamamoto, Yasuhito Ishigaki, Shigetaka Shimodaira, Yusuke Shimizu, Toru Ichiseki, Ayumi Kaneuji, Satoshi Osawa, Norio Kawahara

Research output: Contribution to journalArticlepeer-review

Abstract

Osteoarthritis (OA) is an irreversible degenerative condition causing bone deformation in the joints and articular cartilage degeneration with chronic pain and impaired movement. Adipose-derived stem cell (ADSC) or crushed adipose tissue injection into the joint cavity reportedly improve knee function and symptoms, including pain. Stem cell spheroids may be promising treatment options due to their anti-inflammatory and enhanced tissue regeneration/repair effects. Herein, to form human ADSC spheroids, we used first SphereRing® (Fukoku Co., Ltd., Ageo, Japan), a newly developed rotating donut-shaped tube and determined their characteristics by DNA microarray of mRNA analysis. The variable gene expression cluster was then identified and validated by RT-PCR. Gene expression fluctuations were observed, such as COL15A1 and ANGPTL2, related to vascular endothelial cells and angiogenesis, and TNC, involved in tissue formation. In addition, multiplex cytokine analysis in the medium revealed significant cytokines and growth factors production increase of IL-6, IL-10, etc. However, ADSC administration into the joint cavity involves their contact with the synovial fluid (SF). Therefore, we examined how SF collected from OA patient joint cavities affect 2D-culture ADSCs and ADSC spheroids and observed SF induced cell death. ADSC spheroids could become promising OA treatment options, although studying the administration methods and consider their interaction with SF is essential.

Original languageEnglish
Article number337
JournalCells
Volume11
Issue number3
DOIs
Publication statusPublished - 01-02-2022

All Science Journal Classification (ASJC) codes

  • Medicine(all)

Fingerprint

Dive into the research topics of 'Evaluation of the Usefulness of Human Adipose-Derived Stem Cell Spheroids Formed Using SphereRing® and the Lethal Damage Sensitivity to Synovial Fluid In Vitro'. Together they form a unique fingerprint.

Cite this