Evaluation of two different homogeneous assays for LDL-cholesterol in lipoprotein-X-positive serum

H. Fei, S. Maeda, H. Kirii, S. Fujigaki, N. Maekawa, H. Fujii, H. Wada, K. Saito, M. Seishima

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

Background: The purpose of this study was to evaluate the performance of two homogeneous assays for LDL-cholesterol (LDL-C), a polyethylene/cyclodextrin (PC) assay and a detergent (D) assay, which are based on different principles, in cholestatic serum. Methods: We compared serum LDL-C concentrations determined by the two assays for healthy normolipidemic subjects (n = 42) and cholestatic patients (n = 51). LDL-C concentrations obtained with the homogeneous assays were also compared with those obtained by HPLC for patients' sera. In the interference study, conjugated bile acids were added to normal serum, and their effects on the two assays were examined. The effects of lipoprotein-X (LP-X), intermediate-density lipoprotein (IDL), and apolipoprotein (apo) E-rich HDL on the LDL-C assays were also investigated by adding these lipoproteins to normal serum. Results: The LDL-C concentrations obtained with the D assay were higher than those obtained with the PC assay in the serum with high LP-X. The bias for LDL-C vs LP-X in cholestatic serum correlated with LP-X concentration (r = 0.582; P <0.0001; n = 51). In the interference study, no effect of bile acids on the LDL-C assays was observed. However, the D assay measured 51.0% of the cholesterol in LP-X, whereas no reactivity was observed for LP-X in the PC assay. In addition, the D assay and the PC assay measured IDL-cholesterol at 31.2% and 52.4%, respectively, and measured apo E-rich HDL-C at 7.6% and 17.8%, respectively. Conclusions: Although both homogeneous LDL-C assays are suitable for most cases, the present study showed that each homogeneous assay has a different limitation for cholestatic serum with gross alterations in lipoproteins. (C) 2000 American Association for Clinical Chemistry.

Original languageEnglish
Pages (from-to)1351-1356
Number of pages6
JournalClinical Chemistry
Volume46
Issue number9
Publication statusPublished - 26-09-2000
Externally publishedYes

Fingerprint

Lipoprotein-X
LDL Cholesterol
Assays
Cyclodextrins
Polyethylene
Serum
Bile Acids and Salts
Lipoproteins
IDL Lipoproteins
low density lipoprotein inhibitor
Detergents
Healthy Volunteers
High Pressure Liquid Chromatography

All Science Journal Classification (ASJC) codes

  • Clinical Biochemistry
  • Biochemistry, medical

Cite this

Fei, H., Maeda, S., Kirii, H., Fujigaki, S., Maekawa, N., Fujii, H., ... Seishima, M. (2000). Evaluation of two different homogeneous assays for LDL-cholesterol in lipoprotein-X-positive serum. Clinical Chemistry, 46(9), 1351-1356.
Fei, H. ; Maeda, S. ; Kirii, H. ; Fujigaki, S. ; Maekawa, N. ; Fujii, H. ; Wada, H. ; Saito, K. ; Seishima, M. / Evaluation of two different homogeneous assays for LDL-cholesterol in lipoprotein-X-positive serum. In: Clinical Chemistry. 2000 ; Vol. 46, No. 9. pp. 1351-1356.
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Fei, H, Maeda, S, Kirii, H, Fujigaki, S, Maekawa, N, Fujii, H, Wada, H, Saito, K & Seishima, M 2000, 'Evaluation of two different homogeneous assays for LDL-cholesterol in lipoprotein-X-positive serum', Clinical Chemistry, vol. 46, no. 9, pp. 1351-1356.

Evaluation of two different homogeneous assays for LDL-cholesterol in lipoprotein-X-positive serum. / Fei, H.; Maeda, S.; Kirii, H.; Fujigaki, S.; Maekawa, N.; Fujii, H.; Wada, H.; Saito, K.; Seishima, M.

In: Clinical Chemistry, Vol. 46, No. 9, 26.09.2000, p. 1351-1356.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Evaluation of two different homogeneous assays for LDL-cholesterol in lipoprotein-X-positive serum

AU - Fei, H.

AU - Maeda, S.

AU - Kirii, H.

AU - Fujigaki, S.

AU - Maekawa, N.

AU - Fujii, H.

AU - Wada, H.

AU - Saito, K.

AU - Seishima, M.

PY - 2000/9/26

Y1 - 2000/9/26

N2 - Background: The purpose of this study was to evaluate the performance of two homogeneous assays for LDL-cholesterol (LDL-C), a polyethylene/cyclodextrin (PC) assay and a detergent (D) assay, which are based on different principles, in cholestatic serum. Methods: We compared serum LDL-C concentrations determined by the two assays for healthy normolipidemic subjects (n = 42) and cholestatic patients (n = 51). LDL-C concentrations obtained with the homogeneous assays were also compared with those obtained by HPLC for patients' sera. In the interference study, conjugated bile acids were added to normal serum, and their effects on the two assays were examined. The effects of lipoprotein-X (LP-X), intermediate-density lipoprotein (IDL), and apolipoprotein (apo) E-rich HDL on the LDL-C assays were also investigated by adding these lipoproteins to normal serum. Results: The LDL-C concentrations obtained with the D assay were higher than those obtained with the PC assay in the serum with high LP-X. The bias for LDL-C vs LP-X in cholestatic serum correlated with LP-X concentration (r = 0.582; P <0.0001; n = 51). In the interference study, no effect of bile acids on the LDL-C assays was observed. However, the D assay measured 51.0% of the cholesterol in LP-X, whereas no reactivity was observed for LP-X in the PC assay. In addition, the D assay and the PC assay measured IDL-cholesterol at 31.2% and 52.4%, respectively, and measured apo E-rich HDL-C at 7.6% and 17.8%, respectively. Conclusions: Although both homogeneous LDL-C assays are suitable for most cases, the present study showed that each homogeneous assay has a different limitation for cholestatic serum with gross alterations in lipoproteins. (C) 2000 American Association for Clinical Chemistry.

AB - Background: The purpose of this study was to evaluate the performance of two homogeneous assays for LDL-cholesterol (LDL-C), a polyethylene/cyclodextrin (PC) assay and a detergent (D) assay, which are based on different principles, in cholestatic serum. Methods: We compared serum LDL-C concentrations determined by the two assays for healthy normolipidemic subjects (n = 42) and cholestatic patients (n = 51). LDL-C concentrations obtained with the homogeneous assays were also compared with those obtained by HPLC for patients' sera. In the interference study, conjugated bile acids were added to normal serum, and their effects on the two assays were examined. The effects of lipoprotein-X (LP-X), intermediate-density lipoprotein (IDL), and apolipoprotein (apo) E-rich HDL on the LDL-C assays were also investigated by adding these lipoproteins to normal serum. Results: The LDL-C concentrations obtained with the D assay were higher than those obtained with the PC assay in the serum with high LP-X. The bias for LDL-C vs LP-X in cholestatic serum correlated with LP-X concentration (r = 0.582; P <0.0001; n = 51). In the interference study, no effect of bile acids on the LDL-C assays was observed. However, the D assay measured 51.0% of the cholesterol in LP-X, whereas no reactivity was observed for LP-X in the PC assay. In addition, the D assay and the PC assay measured IDL-cholesterol at 31.2% and 52.4%, respectively, and measured apo E-rich HDL-C at 7.6% and 17.8%, respectively. Conclusions: Although both homogeneous LDL-C assays are suitable for most cases, the present study showed that each homogeneous assay has a different limitation for cholestatic serum with gross alterations in lipoproteins. (C) 2000 American Association for Clinical Chemistry.

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Fei H, Maeda S, Kirii H, Fujigaki S, Maekawa N, Fujii H et al. Evaluation of two different homogeneous assays for LDL-cholesterol in lipoprotein-X-positive serum. Clinical Chemistry. 2000 Sep 26;46(9):1351-1356.