Abstract
The potential toxicity resulting from combinatorial effects of organophosphorus and pyrethroid insecticides are not completely known. We evaluated male reproductive toxicity in mice co-exposed to diazinon and cis-permethrin. Nine-week-old male Sv/129 mice were exposed to diazinon (10 μmol/kg/day) or cis-permethrin (90 μmol/kg/day) alone or in combination (100 μmol/kg/day), or vehicle (corn oil), for 6 weeks. Diazinon and the diazinon-permethrin mixture inhibited plasma and liver carboxylesterase activities. In the mixture group, urinary excretion of cis-permethrin metabolite 3-phenoxybenzoic acid decreased along with increased plasma and testicular concentrations of cis-permethrin, while excretion of diazinon metabolites, diethylphosphate and diethylthiophosphate, did not change, versus mice exposed to each chemical alone, which suggested that inhibition of carboxylesterase decreased the metabolic capacity to cis-permethrin. Though the co-exposure decreased testosterone biosynthesis, increased degenerate germ cells in seminiferous tubule and sperm morphological abnormalities versus controls more clearly than exposure to cis-permethrin alone, the expected potentiation of toxicity was not evident.
| Original language | English |
|---|---|
| Pages (from-to) | 489-497 |
| Number of pages | 9 |
| Journal | Reproductive Toxicology |
| Volume | 34 |
| Issue number | 4 |
| DOIs | |
| Publication status | Published - 01-12-2012 |
| Externally published | Yes |
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All Science Journal Classification (ASJC) codes
- Toxicology
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Evidence for diazinon-mediated inhibition of cis-permethrin metabolism and its effects on reproductive toxicity in adult male mice. / Wang, Dong; Kamijima, Michihiro; Okamura, Ai; Ito, Yuki; Yanagiba, Yukie; Jia, Xiao Fang; Naito, Hisao; Ueyama, Jun; Nakajima, Tamie.
In: Reproductive Toxicology, Vol. 34, No. 4, 01.12.2012, p. 489-497.Research output: Contribution to journal › Article
TY - JOUR
T1 - Evidence for diazinon-mediated inhibition of cis-permethrin metabolism and its effects on reproductive toxicity in adult male mice
AU - Wang, Dong
AU - Kamijima, Michihiro
AU - Okamura, Ai
AU - Ito, Yuki
AU - Yanagiba, Yukie
AU - Jia, Xiao Fang
AU - Naito, Hisao
AU - Ueyama, Jun
AU - Nakajima, Tamie
PY - 2012/12/1
Y1 - 2012/12/1
N2 - The potential toxicity resulting from combinatorial effects of organophosphorus and pyrethroid insecticides are not completely known. We evaluated male reproductive toxicity in mice co-exposed to diazinon and cis-permethrin. Nine-week-old male Sv/129 mice were exposed to diazinon (10 μmol/kg/day) or cis-permethrin (90 μmol/kg/day) alone or in combination (100 μmol/kg/day), or vehicle (corn oil), for 6 weeks. Diazinon and the diazinon-permethrin mixture inhibited plasma and liver carboxylesterase activities. In the mixture group, urinary excretion of cis-permethrin metabolite 3-phenoxybenzoic acid decreased along with increased plasma and testicular concentrations of cis-permethrin, while excretion of diazinon metabolites, diethylphosphate and diethylthiophosphate, did not change, versus mice exposed to each chemical alone, which suggested that inhibition of carboxylesterase decreased the metabolic capacity to cis-permethrin. Though the co-exposure decreased testosterone biosynthesis, increased degenerate germ cells in seminiferous tubule and sperm morphological abnormalities versus controls more clearly than exposure to cis-permethrin alone, the expected potentiation of toxicity was not evident.
AB - The potential toxicity resulting from combinatorial effects of organophosphorus and pyrethroid insecticides are not completely known. We evaluated male reproductive toxicity in mice co-exposed to diazinon and cis-permethrin. Nine-week-old male Sv/129 mice were exposed to diazinon (10 μmol/kg/day) or cis-permethrin (90 μmol/kg/day) alone or in combination (100 μmol/kg/day), or vehicle (corn oil), for 6 weeks. Diazinon and the diazinon-permethrin mixture inhibited plasma and liver carboxylesterase activities. In the mixture group, urinary excretion of cis-permethrin metabolite 3-phenoxybenzoic acid decreased along with increased plasma and testicular concentrations of cis-permethrin, while excretion of diazinon metabolites, diethylphosphate and diethylthiophosphate, did not change, versus mice exposed to each chemical alone, which suggested that inhibition of carboxylesterase decreased the metabolic capacity to cis-permethrin. Though the co-exposure decreased testosterone biosynthesis, increased degenerate germ cells in seminiferous tubule and sperm morphological abnormalities versus controls more clearly than exposure to cis-permethrin alone, the expected potentiation of toxicity was not evident.
UR - http://www.scopus.com/inward/record.url?scp=84865559153&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84865559153&partnerID=8YFLogxK
U2 - 10.1016/j.reprotox.2012.07.007
DO - 10.1016/j.reprotox.2012.07.007
M3 - Article
C2 - 22944209
AN - SCOPUS:84865559153
VL - 34
SP - 489
EP - 497
JO - Reproductigve Toxicoloy
JF - Reproductigve Toxicoloy
SN - 0890-6238
IS - 4
ER -