Exclusion of CD43 from the immunological synapce is mediated by phosphorylation-regulated relocation of the cytoskeletal adaptor Moesin

Jérôme Delon, Kozo Kaibuchi, Ronald N. Germain

Research output: Contribution to journalArticlepeer-review

218 Citations (Scopus)

Abstract

Formation of the immunological synapse requires TCR signal-dependent protein redistribution. However, the specific molecular mechanisms controlling protein relocation are not well defined. Moesin is a widely expressed phospho-protein that links many transmembrane molecules to the cortical actin cytoskeleton. Here, we demonstrate that TCR-induced exclusion of the large sialoprotein CD43 from the synapse is an active event mediated by its reversible binding to moesin. Our results also reveal that relocalization of moesin is associated with changes in the phosphorylation status of this cytoskeletal adaptor protein. Finally, these findings raise the possibility that the change in moesin localization resulting from TCR engagement modifies the overall topology of the lymphocyte membrane and facilitates molecular interactions at the site of presenting cell contact.

Original languageEnglish
Pages (from-to)691-701
Number of pages11
JournalImmunity
Volume15
Issue number5
DOIs
Publication statusPublished - 2001

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology
  • Infectious Diseases

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