Expansion of extrathymic T cells as well as granulocytes in the liver and other organs of granulocyte-colony stimulating factor transgenic mice: Why they lost the ability of hybrid resistance

Hiroki Kawamura, Toshihiko Kawamura, Yasuo Kokai, Michio Mori, Akihiro Matsuura, Hiroshi Oya, Shigeru Honda, Susumu Suzuki, Anura Weerashinghe, Hisami Watanabe, Toru Abo

Research output: Contribution to journalArticle

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Abstract

When we attempted to characterize the immunological state in G-CSF transgenic mice, a large number of not only granulocytes but also lymphoid cells expanded in various immune organs. Such lymphoid cells were present at unusual sites of these organs, e.g., the parenchymal space in the liver. We then determined the phenotype of these lymphoid cells by immunofluorescence tests. It was demonstrated that CD3(int)IL-2Rβ+ cells (i.e., extrathymic T cells), including the NK1.1+ subset of CD3(int) cells (i.e., NKT cells), increased in the liver and all other tested organs. These T cells as well as NK cells mediated NK and NK-like cytotoxicity, especially at youth. However, they were not able to mediate such cytotoxicity in the presence of granulocytes. This result might be associated with deficiency in the hybrid resistance previously ascribed to these mice. In other words, G-CSF transgenic mice had a large number of extrathymic T cells (including NKT cells) and NK cells that mediate hybrid resistance, but their function was suppressed by activated granulocytes. Indeed, these granulocytes showed an elevated level of Ca2+ influx upon stimulation. The present results suggest that, in parallel with overactivation of granulocytes, extrathymic T cells and NK cells are concomitantly activated in number but that their function is suppressed in G-CSF transgenic mice.

Original languageEnglish
Pages (from-to)5957-5964
Number of pages8
JournalJournal of Immunology
Volume162
Issue number10
Publication statusPublished - 15-05-1999

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Granulocyte Colony-Stimulating Factor
Granulocytes
Transgenic Mice
T-Lymphocytes
Natural Killer Cells
Liver
Natural Killer T-Cells
Lymphocytes
Fluorescent Antibody Technique
Phenotype

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology

Cite this

Kawamura, Hiroki ; Kawamura, Toshihiko ; Kokai, Yasuo ; Mori, Michio ; Matsuura, Akihiro ; Oya, Hiroshi ; Honda, Shigeru ; Suzuki, Susumu ; Weerashinghe, Anura ; Watanabe, Hisami ; Abo, Toru. / Expansion of extrathymic T cells as well as granulocytes in the liver and other organs of granulocyte-colony stimulating factor transgenic mice : Why they lost the ability of hybrid resistance. In: Journal of Immunology. 1999 ; Vol. 162, No. 10. pp. 5957-5964.
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abstract = "When we attempted to characterize the immunological state in G-CSF transgenic mice, a large number of not only granulocytes but also lymphoid cells expanded in various immune organs. Such lymphoid cells were present at unusual sites of these organs, e.g., the parenchymal space in the liver. We then determined the phenotype of these lymphoid cells by immunofluorescence tests. It was demonstrated that CD3(int)IL-2Rβ+ cells (i.e., extrathymic T cells), including the NK1.1+ subset of CD3(int) cells (i.e., NKT cells), increased in the liver and all other tested organs. These T cells as well as NK cells mediated NK and NK-like cytotoxicity, especially at youth. However, they were not able to mediate such cytotoxicity in the presence of granulocytes. This result might be associated with deficiency in the hybrid resistance previously ascribed to these mice. In other words, G-CSF transgenic mice had a large number of extrathymic T cells (including NKT cells) and NK cells that mediate hybrid resistance, but their function was suppressed by activated granulocytes. Indeed, these granulocytes showed an elevated level of Ca2+ influx upon stimulation. The present results suggest that, in parallel with overactivation of granulocytes, extrathymic T cells and NK cells are concomitantly activated in number but that their function is suppressed in G-CSF transgenic mice.",
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Kawamura, H, Kawamura, T, Kokai, Y, Mori, M, Matsuura, A, Oya, H, Honda, S, Suzuki, S, Weerashinghe, A, Watanabe, H & Abo, T 1999, 'Expansion of extrathymic T cells as well as granulocytes in the liver and other organs of granulocyte-colony stimulating factor transgenic mice: Why they lost the ability of hybrid resistance', Journal of Immunology, vol. 162, no. 10, pp. 5957-5964.

Expansion of extrathymic T cells as well as granulocytes in the liver and other organs of granulocyte-colony stimulating factor transgenic mice : Why they lost the ability of hybrid resistance. / Kawamura, Hiroki; Kawamura, Toshihiko; Kokai, Yasuo; Mori, Michio; Matsuura, Akihiro; Oya, Hiroshi; Honda, Shigeru; Suzuki, Susumu; Weerashinghe, Anura; Watanabe, Hisami; Abo, Toru.

In: Journal of Immunology, Vol. 162, No. 10, 15.05.1999, p. 5957-5964.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Expansion of extrathymic T cells as well as granulocytes in the liver and other organs of granulocyte-colony stimulating factor transgenic mice

T2 - Why they lost the ability of hybrid resistance

AU - Kawamura, Hiroki

AU - Kawamura, Toshihiko

AU - Kokai, Yasuo

AU - Mori, Michio

AU - Matsuura, Akihiro

AU - Oya, Hiroshi

AU - Honda, Shigeru

AU - Suzuki, Susumu

AU - Weerashinghe, Anura

AU - Watanabe, Hisami

AU - Abo, Toru

PY - 1999/5/15

Y1 - 1999/5/15

N2 - When we attempted to characterize the immunological state in G-CSF transgenic mice, a large number of not only granulocytes but also lymphoid cells expanded in various immune organs. Such lymphoid cells were present at unusual sites of these organs, e.g., the parenchymal space in the liver. We then determined the phenotype of these lymphoid cells by immunofluorescence tests. It was demonstrated that CD3(int)IL-2Rβ+ cells (i.e., extrathymic T cells), including the NK1.1+ subset of CD3(int) cells (i.e., NKT cells), increased in the liver and all other tested organs. These T cells as well as NK cells mediated NK and NK-like cytotoxicity, especially at youth. However, they were not able to mediate such cytotoxicity in the presence of granulocytes. This result might be associated with deficiency in the hybrid resistance previously ascribed to these mice. In other words, G-CSF transgenic mice had a large number of extrathymic T cells (including NKT cells) and NK cells that mediate hybrid resistance, but their function was suppressed by activated granulocytes. Indeed, these granulocytes showed an elevated level of Ca2+ influx upon stimulation. The present results suggest that, in parallel with overactivation of granulocytes, extrathymic T cells and NK cells are concomitantly activated in number but that their function is suppressed in G-CSF transgenic mice.

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