TY - JOUR
T1 - Experimental Induction of Uterine Cancer in Rats by N-ethyl-N'-nitro-N-nitrosoguanidine Dissolved in Polyethylene Glycol
AU - Ogino, H.
AU - Fujimoto, M.
AU - Oshiro, H.
AU - Matsumoto, K.
AU - Funahashi, M.
AU - Kaneko, C.
AU - Hirono, I.
N1 - Funding Information:
This study was supported by Research Grant from the FujitaGakuen Health University.
PY - 1989
Y1 - 1989
N2 - In the present experiment we attempted to experimentally induce uterine cancer in rats by injecting into the uterine cavity N-ethyl-N'-nitro-N-nitrosoguanidine (ENNG) dissolved in polyethylene glycol (PEG). Fifty-nine female F-344 rats, 7–8 weeks old, were divided into three groups and each received in the left uterine cavity with laparotomy a single dose of ENNG dissolved in PEG according to the following schedule: Group 1 received 75 mg ENNG/kg body wt.; Group 2 had 20 mg ENNG/kg body wt.: and Group 3 was given only PEG. In Group 1 it was observed that adenocarcinoma and sarcoma were present in the uterine corpus while squamous cell carcinoma occurred in the uterine cervix. In Group 2, although tumors such as adenocarcinoma, adenoma and sarcoma were observed in the uterine corpus, no tumor was present in the uterine cervix. No tumor growth whatsoever was observed in Group 3. From the above results it is apparent that the present method is an efficient means for experimentally inducing uterine cancer and that the site of tumor generation varies according to the concentration of ENNG administered.
AB - In the present experiment we attempted to experimentally induce uterine cancer in rats by injecting into the uterine cavity N-ethyl-N'-nitro-N-nitrosoguanidine (ENNG) dissolved in polyethylene glycol (PEG). Fifty-nine female F-344 rats, 7–8 weeks old, were divided into three groups and each received in the left uterine cavity with laparotomy a single dose of ENNG dissolved in PEG according to the following schedule: Group 1 received 75 mg ENNG/kg body wt.; Group 2 had 20 mg ENNG/kg body wt.: and Group 3 was given only PEG. In Group 1 it was observed that adenocarcinoma and sarcoma were present in the uterine corpus while squamous cell carcinoma occurred in the uterine cervix. In Group 2, although tumors such as adenocarcinoma, adenoma and sarcoma were observed in the uterine corpus, no tumor was present in the uterine cervix. No tumor growth whatsoever was observed in Group 3. From the above results it is apparent that the present method is an efficient means for experimentally inducing uterine cancer and that the site of tumor generation varies according to the concentration of ENNG administered.
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U2 - 10.1016/S0344-0338(89)80254-7
DO - 10.1016/S0344-0338(89)80254-7
M3 - Article
C2 - 2798222
AN - SCOPUS:0024378220
SN - 0344-0338
VL - 185
SP - 214
EP - 217
JO - Pathology Research and Practice
JF - Pathology Research and Practice
IS - 2
ER -
