Experimental research on stimulation of wound healing by n-3 fatty acids

Akihiko Futamura, Takashi Higashiguchi, Akihiro Ito, Yoshiyuki Kodama, Takeshi Chihara, Takaaki Kaneko, Akiko Tomatsu, Kan Shimpo

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Objexctive. The objective of this study was to investigate the wound healing effects of n-3 fatty acids and to identify factors that stimulate wound healing. Materials and Methods. Four-week-old male Wistar rats were subjected to full-thickness skin wounds and assigned to 3 experimental diet groups (an n-3 fatty acid-fortified diet, a diet with a 1:3 ratio of n-3 to n-6 fatty acids, and an n-6 fatty acid-fortified diet). Intergroup comparisons were conducted for the changes in wound areas, the number of days to wound healing, and blood cytokines, blood hydroxyproline, and blood chemistry test values on the day before and after wound healing. Results. The number of days to wound healing in the n-3/n-6 fatty acid group (18.4 ± 1.8 days) was significantly shorter than in the n-3 fatty acid-fortified diet (21.6 ± 1.6 days) and n-6 fatty acid-fortified diet groups (21.9 ± 1.8 days). This suggests that the n-3/n-6 fatty acid diet stimulates wound healing (P < 0.05). Changes in wound area, however, were not significantly different. The n-3 fatty acid-fortified diet was found to have potent immunopotentiating and anti-inflammatory effects in the group receiving this diet, as evidenced by total blood lymphocyte count and plasma levels of interleukin-1 beta (IL-1β) and sialic acid on day 1 after wounding. The plasma hydroxyproline concentrations noted in the groups with a diet containing n-3 fatty acids indicate that this fatty acid type stimulates wound healing. Conclusions. Findings suggest that n-3 fatty acids have anti-inflammatory and immunopotentiating effects, and are beneficial in the wound healing process, particularly during early inflammation.

Original languageEnglish
Pages (from-to)186-192
Number of pages7
JournalWounds
Volume25
Issue number7
Publication statusPublished - 01-07-2013

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Omega-3 Fatty Acids
Wound Healing
Diet
Research
Fatty Acids
Hydroxyproline
Wounds and Injuries
Anti-Inflammatory Agents
Lymphocyte Count
Hematologic Tests
N-Acetylneuraminic Acid
Interleukin-1beta
Wistar Rats
Cytokines
Inflammation
Skin

All Science Journal Classification (ASJC) codes

  • Surgery
  • Medical–Surgical

Cite this

Futamura, A., Higashiguchi, T., Ito, A., Kodama, Y., Chihara, T., Kaneko, T., ... Shimpo, K. (2013). Experimental research on stimulation of wound healing by n-3 fatty acids. Wounds, 25(7), 186-192.
Futamura, Akihiko ; Higashiguchi, Takashi ; Ito, Akihiro ; Kodama, Yoshiyuki ; Chihara, Takeshi ; Kaneko, Takaaki ; Tomatsu, Akiko ; Shimpo, Kan. / Experimental research on stimulation of wound healing by n-3 fatty acids. In: Wounds. 2013 ; Vol. 25, No. 7. pp. 186-192.
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abstract = "Objexctive. The objective of this study was to investigate the wound healing effects of n-3 fatty acids and to identify factors that stimulate wound healing. Materials and Methods. Four-week-old male Wistar rats were subjected to full-thickness skin wounds and assigned to 3 experimental diet groups (an n-3 fatty acid-fortified diet, a diet with a 1:3 ratio of n-3 to n-6 fatty acids, and an n-6 fatty acid-fortified diet). Intergroup comparisons were conducted for the changes in wound areas, the number of days to wound healing, and blood cytokines, blood hydroxyproline, and blood chemistry test values on the day before and after wound healing. Results. The number of days to wound healing in the n-3/n-6 fatty acid group (18.4 ± 1.8 days) was significantly shorter than in the n-3 fatty acid-fortified diet (21.6 ± 1.6 days) and n-6 fatty acid-fortified diet groups (21.9 ± 1.8 days). This suggests that the n-3/n-6 fatty acid diet stimulates wound healing (P < 0.05). Changes in wound area, however, were not significantly different. The n-3 fatty acid-fortified diet was found to have potent immunopotentiating and anti-inflammatory effects in the group receiving this diet, as evidenced by total blood lymphocyte count and plasma levels of interleukin-1 beta (IL-1β) and sialic acid on day 1 after wounding. The plasma hydroxyproline concentrations noted in the groups with a diet containing n-3 fatty acids indicate that this fatty acid type stimulates wound healing. Conclusions. Findings suggest that n-3 fatty acids have anti-inflammatory and immunopotentiating effects, and are beneficial in the wound healing process, particularly during early inflammation.",
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Futamura, A, Higashiguchi, T, Ito, A, Kodama, Y, Chihara, T, Kaneko, T, Tomatsu, A & Shimpo, K 2013, 'Experimental research on stimulation of wound healing by n-3 fatty acids', Wounds, vol. 25, no. 7, pp. 186-192.

Experimental research on stimulation of wound healing by n-3 fatty acids. / Futamura, Akihiko; Higashiguchi, Takashi; Ito, Akihiro; Kodama, Yoshiyuki; Chihara, Takeshi; Kaneko, Takaaki; Tomatsu, Akiko; Shimpo, Kan.

In: Wounds, Vol. 25, No. 7, 01.07.2013, p. 186-192.

Research output: Contribution to journalArticle

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T1 - Experimental research on stimulation of wound healing by n-3 fatty acids

AU - Futamura, Akihiko

AU - Higashiguchi, Takashi

AU - Ito, Akihiro

AU - Kodama, Yoshiyuki

AU - Chihara, Takeshi

AU - Kaneko, Takaaki

AU - Tomatsu, Akiko

AU - Shimpo, Kan

PY - 2013/7/1

Y1 - 2013/7/1

N2 - Objexctive. The objective of this study was to investigate the wound healing effects of n-3 fatty acids and to identify factors that stimulate wound healing. Materials and Methods. Four-week-old male Wistar rats were subjected to full-thickness skin wounds and assigned to 3 experimental diet groups (an n-3 fatty acid-fortified diet, a diet with a 1:3 ratio of n-3 to n-6 fatty acids, and an n-6 fatty acid-fortified diet). Intergroup comparisons were conducted for the changes in wound areas, the number of days to wound healing, and blood cytokines, blood hydroxyproline, and blood chemistry test values on the day before and after wound healing. Results. The number of days to wound healing in the n-3/n-6 fatty acid group (18.4 ± 1.8 days) was significantly shorter than in the n-3 fatty acid-fortified diet (21.6 ± 1.6 days) and n-6 fatty acid-fortified diet groups (21.9 ± 1.8 days). This suggests that the n-3/n-6 fatty acid diet stimulates wound healing (P < 0.05). Changes in wound area, however, were not significantly different. The n-3 fatty acid-fortified diet was found to have potent immunopotentiating and anti-inflammatory effects in the group receiving this diet, as evidenced by total blood lymphocyte count and plasma levels of interleukin-1 beta (IL-1β) and sialic acid on day 1 after wounding. The plasma hydroxyproline concentrations noted in the groups with a diet containing n-3 fatty acids indicate that this fatty acid type stimulates wound healing. Conclusions. Findings suggest that n-3 fatty acids have anti-inflammatory and immunopotentiating effects, and are beneficial in the wound healing process, particularly during early inflammation.

AB - Objexctive. The objective of this study was to investigate the wound healing effects of n-3 fatty acids and to identify factors that stimulate wound healing. Materials and Methods. Four-week-old male Wistar rats were subjected to full-thickness skin wounds and assigned to 3 experimental diet groups (an n-3 fatty acid-fortified diet, a diet with a 1:3 ratio of n-3 to n-6 fatty acids, and an n-6 fatty acid-fortified diet). Intergroup comparisons were conducted for the changes in wound areas, the number of days to wound healing, and blood cytokines, blood hydroxyproline, and blood chemistry test values on the day before and after wound healing. Results. The number of days to wound healing in the n-3/n-6 fatty acid group (18.4 ± 1.8 days) was significantly shorter than in the n-3 fatty acid-fortified diet (21.6 ± 1.6 days) and n-6 fatty acid-fortified diet groups (21.9 ± 1.8 days). This suggests that the n-3/n-6 fatty acid diet stimulates wound healing (P < 0.05). Changes in wound area, however, were not significantly different. The n-3 fatty acid-fortified diet was found to have potent immunopotentiating and anti-inflammatory effects in the group receiving this diet, as evidenced by total blood lymphocyte count and plasma levels of interleukin-1 beta (IL-1β) and sialic acid on day 1 after wounding. The plasma hydroxyproline concentrations noted in the groups with a diet containing n-3 fatty acids indicate that this fatty acid type stimulates wound healing. Conclusions. Findings suggest that n-3 fatty acids have anti-inflammatory and immunopotentiating effects, and are beneficial in the wound healing process, particularly during early inflammation.

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