TY - JOUR
T1 - Expression analysis of a mouse orthologue of HSFY, a candidate for the azoospermic factor on the human Y chromosome
AU - Kinoshita, Keigo
AU - Shinka, Toshikatsu
AU - Sato, Youichi
AU - Kurahashi, Hiroki
AU - Kowa, Hiroe
AU - Chen, Gang
AU - Umeno, Mayumi
AU - Toida, Kazunori
AU - Kiyokage, Emi
AU - Nakano, Takuro
AU - Ito, Susumu
AU - Nakahori, Yutaka
PY - 2006/2
Y1 - 2006/2
N2 - Heat shock transcription factor on Y (HSFY) is located in one of three candidate regions for azoospermic factor (AZF), AZFb on the Y chromosome. We and others have already revealed that some azoospermic males are missing the regions of the Y chromosome including HSFY. Previously, we showed that murine HSFY-like sequence (mHSFYL (Riken cDNA 4933413G11Rik)), which is the mouse orthologue of HSFY, is exclusively expressed in testis. The sequences encoding the presumed DNA-binding domain in HSFY and mHSFYL were found in other mammals such as dogs, cows and chickens. To elucidate mHSFYL expression in the testes in detail, we carried out in situ hybridization. mHSFYL was predominantly expressed in round spermatids. Furthermore, we clarified the intracellular distribution of mHSFYL in COS1 cells with HA-or GFP-tagged proteins. Both HA-mHSFYL and GFP-mHSFYL were located in the nucleus. Our results suggest that HSFY/mHSFYL may have evolutionarily conserved functions for spermatogenesis.
AB - Heat shock transcription factor on Y (HSFY) is located in one of three candidate regions for azoospermic factor (AZF), AZFb on the Y chromosome. We and others have already revealed that some azoospermic males are missing the regions of the Y chromosome including HSFY. Previously, we showed that murine HSFY-like sequence (mHSFYL (Riken cDNA 4933413G11Rik)), which is the mouse orthologue of HSFY, is exclusively expressed in testis. The sequences encoding the presumed DNA-binding domain in HSFY and mHSFYL were found in other mammals such as dogs, cows and chickens. To elucidate mHSFYL expression in the testes in detail, we carried out in situ hybridization. mHSFYL was predominantly expressed in round spermatids. Furthermore, we clarified the intracellular distribution of mHSFYL in COS1 cells with HA-or GFP-tagged proteins. Both HA-mHSFYL and GFP-mHSFYL were located in the nucleus. Our results suggest that HSFY/mHSFYL may have evolutionarily conserved functions for spermatogenesis.
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U2 - 10.2152/jmi.53.117
DO - 10.2152/jmi.53.117
M3 - Article
C2 - 16538004
AN - SCOPUS:33645798670
SN - 1343-1420
VL - 53
SP - 117
EP - 122
JO - Journal of Medical Investigation
JF - Journal of Medical Investigation
IS - 1-2
ER -