Expression analysis of gibberellin-regulated protein in peach by reverse transcription-quantitative PCR

Natsuki Kawai, Yuki Hirakawa, Shinya Matsumoto, Akihiro Itai, Kayoko Matsunaga, Hiroshi Narita, Keiko Momma

Research output: Contribution to journalArticlepeer-review

Abstract

Gibberellin-regulated protein (GRP) is a fruit severe allergen. The amounts of GRP expression normalized against actin in peach were determined by reverse transcription-quantitative PCR (RT-qPCR). The results were consistent with those determined by enzyme-linked immunosorbent assay (ELISA). The GRP expression was more evident in flesh than peel and increased rapidly in the maturing period. This approach is applicable to estimate the amount of GRP in other plants.

Original languageEnglish
Pages (from-to)1459-1461
Number of pages3
JournalBioscience, Biotechnology, and Biochemistry
Volume86
Issue number10
DOIs
Publication statusPublished - 23-09-2022

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Analytical Chemistry
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry

Fingerprint

Dive into the research topics of 'Expression analysis of gibberellin-regulated protein in peach by reverse transcription-quantitative PCR'. Together they form a unique fingerprint.

Cite this