Expression analysis of gibberellin-regulated protein in peach by reverse transcription-quantitative PCR

Natsuki Kawai; Yuki Hirakawa; Shinya Matsumoto; Akihiro Itai; Kayoko Matsunaga; Hiroshi Narita; Keiko Momma

doi:10.1093/bbb/zbac123

Expression analysis of gibberellin-regulated protein in peach by reverse transcription-quantitative PCR

Natsuki Kawai, Yuki Hirakawa, Shinya Matsumoto, Akihiro Itai, Kayoko Matsunaga, Hiroshi Narita, Keiko Momma

integrative medical science for allergic disease

Research output: Contribution to journal › Article › peer-review

Abstract

Gibberellin-regulated protein (GRP) is a fruit severe allergen. The amounts of GRP expression normalized against actin in peach were determined by reverse transcription-quantitative PCR (RT-qPCR). The results were consistent with those determined by enzyme-linked immunosorbent assay (ELISA). The GRP expression was more evident in flesh than peel and increased rapidly in the maturing period. This approach is applicable to estimate the amount of GRP in other plants.

Original language	English
Pages (from-to)	1459-1461
Number of pages	3
Journal	Bioscience, Biotechnology and Biochemistry
Volume	86
Issue number	10
DOIs	https://doi.org/10.1093/bbb/zbac123
Publication status	Published - 01-10-2022

All Science Journal Classification (ASJC) codes

General Medicine

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10.1093/bbb/zbac123

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title = "Expression analysis of gibberellin-regulated protein in peach by reverse transcription-quantitative PCR",

abstract = "Gibberellin-regulated protein (GRP) is a fruit severe allergen. The amounts of GRP expression normalized against actin in peach were determined by reverse transcription-quantitative PCR (RT-qPCR). The results were consistent with those determined by enzyme-linked immunosorbent assay (ELISA). The GRP expression was more evident in flesh than peel and increased rapidly in the maturing period. This approach is applicable to estimate the amount of GRP in other plants.",

author = "Natsuki Kawai and Yuki Hirakawa and Shinya Matsumoto and Akihiro Itai and Kayoko Matsunaga and Hiroshi Narita and Keiko Momma",

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AU - Kawai, Natsuki

AU - Hirakawa, Yuki

AU - Matsumoto, Shinya

AU - Itai, Akihiro

AU - Matsunaga, Kayoko

AU - Narita, Hiroshi

AU - Momma, Keiko

PY - 2022/10/1

Y1 - 2022/10/1

N2 - Gibberellin-regulated protein (GRP) is a fruit severe allergen. The amounts of GRP expression normalized against actin in peach were determined by reverse transcription-quantitative PCR (RT-qPCR). The results were consistent with those determined by enzyme-linked immunosorbent assay (ELISA). The GRP expression was more evident in flesh than peel and increased rapidly in the maturing period. This approach is applicable to estimate the amount of GRP in other plants.

AB - Gibberellin-regulated protein (GRP) is a fruit severe allergen. The amounts of GRP expression normalized against actin in peach were determined by reverse transcription-quantitative PCR (RT-qPCR). The results were consistent with those determined by enzyme-linked immunosorbent assay (ELISA). The GRP expression was more evident in flesh than peel and increased rapidly in the maturing period. This approach is applicable to estimate the amount of GRP in other plants.

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IS - 10

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Expression analysis of gibberellin-regulated protein in peach by reverse transcription-quantitative PCR

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