Expression and functional characterization of the system L amino acid transporter in KB human oral epidermoid carcinoma cells

Jung Hoon Yoon; Youn Bae Kim; Myong Soo Kim; Joo Cheol Park; Joong Ki Kook; Hae Man Jung; Saeng Gon Kim; Hoon Yoo; Yeong Mu Ko; Sang Ho Lee; Bong Young Kim; Hong Sung Chun; Yoshikatsu Kanai; Hitoshi Endou; Do Kyung Kim

doi:10.1016/j.canlet.2003.10.009

Expression and functional characterization of the system L amino acid transporter in KB human oral epidermoid carcinoma cells

Jung Hoon Yoon, Youn Bae Kim, Myong Soo Kim, Joo Cheol Park, Joong Ki Kook, Hae Man Jung, Saeng Gon Kim, Hoon Yoo, Yeong Mu Ko, Sang Ho Lee, Bong Young Kim, Hong Sung Chun, Yoshikatsu Kanai, Hitoshi Endou, Do Kyung Kim

Research output: Contribution to journal › Article › peer-review

29 Citations (Scopus)

Abstract

We have examined the expression and function of system L amino acid transporter in KB human oral epidermoid carcinoma cells. The KB cells express L-type amino acid transporter 1 (LAT1) in plasma membrane, but not L-type amino acid transporter 2 (LAT2). The [¹⁴C]L-leucine uptake by KB cells is inhibited by system L selective inhibitor BCH. The majority of [ ¹⁴C]L-leucine uptake is, therefore, mediated by LAT1. These results suggest that the transport of neutral amino acids including several essential amino acids into the KB cells mediated by LAT1 and the specific inhibition of LAT1 in oral cancer cells will be a new rationale for anti-cancer therapy.

Original language	English
Pages (from-to)	215-226
Number of pages	12
Journal	Cancer Letters
Volume	205
Issue number	2
DOIs	https://doi.org/10.1016/j.canlet.2003.10.009
Publication status	Published - 18-03-2004
Externally published	Yes

All Science Journal Classification (ASJC) codes

Oncology
Cancer Research

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.canlet.2003.10.009

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Yoon, J. H., Kim, Y. B., Kim, M. S., Park, J. C., Kook, J. K., Jung, H. M., Kim, S. G., Yoo, H., Ko, Y. M., Lee, S. H., Kim, B. Y., Chun, H. S., Kanai, Y., Endou, H., & Kim, D. K. (2004). Expression and functional characterization of the system L amino acid transporter in KB human oral epidermoid carcinoma cells. Cancer Letters, 205(2), 215-226. https://doi.org/10.1016/j.canlet.2003.10.009

@article{7298945b5e194548a83a0a127665ee9e,

title = "Expression and functional characterization of the system L amino acid transporter in KB human oral epidermoid carcinoma cells",

abstract = "We have examined the expression and function of system L amino acid transporter in KB human oral epidermoid carcinoma cells. The KB cells express L-type amino acid transporter 1 (LAT1) in plasma membrane, but not L-type amino acid transporter 2 (LAT2). The [14C]L-leucine uptake by KB cells is inhibited by system L selective inhibitor BCH. The majority of [ 14C]L-leucine uptake is, therefore, mediated by LAT1. These results suggest that the transport of neutral amino acids including several essential amino acids into the KB cells mediated by LAT1 and the specific inhibition of LAT1 in oral cancer cells will be a new rationale for anti-cancer therapy.",

keywords = "Anti-cancer therapy, Essential amino acids, KB human oral epidermoid carcinoma cells, L-Type amino acid transporter",

author = "Yoon, \{Jung Hoon\} and Kim, \{Youn Bae\} and Kim, \{Myong Soo\} and Park, \{Joo Cheol\} and Kook, \{Joong Ki\} and Jung, \{Hae Man\} and Kim, \{Saeng Gon\} and Hoon Yoo and Ko, \{Yeong Mu\} and Lee, \{Sang Ho\} and Kim, \{Bong Young\} and Chun, \{Hong Sung\} and Yoshikatsu Kanai and Hitoshi Endou and Kim, \{Do Kyung\}",

year = "2004",

month = mar,

day = "18",

doi = "10.1016/j.canlet.2003.10.009",

language = "English",

volume = "205",

pages = "215--226",

journal = "Cancer Letters",

issn = "0304-3835",

publisher = "Elsevier Ireland Ltd",

number = "2",

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TY - JOUR

T1 - Expression and functional characterization of the system L amino acid transporter in KB human oral epidermoid carcinoma cells

AU - Yoon, Jung Hoon

AU - Kim, Youn Bae

AU - Kim, Myong Soo

AU - Park, Joo Cheol

AU - Kook, Joong Ki

AU - Jung, Hae Man

AU - Kim, Saeng Gon

AU - Yoo, Hoon

AU - Ko, Yeong Mu

AU - Lee, Sang Ho

AU - Kim, Bong Young

AU - Chun, Hong Sung

AU - Kanai, Yoshikatsu

AU - Endou, Hitoshi

AU - Kim, Do Kyung

PY - 2004/3/18

Y1 - 2004/3/18

N2 - We have examined the expression and function of system L amino acid transporter in KB human oral epidermoid carcinoma cells. The KB cells express L-type amino acid transporter 1 (LAT1) in plasma membrane, but not L-type amino acid transporter 2 (LAT2). The [14C]L-leucine uptake by KB cells is inhibited by system L selective inhibitor BCH. The majority of [ 14C]L-leucine uptake is, therefore, mediated by LAT1. These results suggest that the transport of neutral amino acids including several essential amino acids into the KB cells mediated by LAT1 and the specific inhibition of LAT1 in oral cancer cells will be a new rationale for anti-cancer therapy.

AB - We have examined the expression and function of system L amino acid transporter in KB human oral epidermoid carcinoma cells. The KB cells express L-type amino acid transporter 1 (LAT1) in plasma membrane, but not L-type amino acid transporter 2 (LAT2). The [14C]L-leucine uptake by KB cells is inhibited by system L selective inhibitor BCH. The majority of [ 14C]L-leucine uptake is, therefore, mediated by LAT1. These results suggest that the transport of neutral amino acids including several essential amino acids into the KB cells mediated by LAT1 and the specific inhibition of LAT1 in oral cancer cells will be a new rationale for anti-cancer therapy.

KW - Anti-cancer therapy

KW - Essential amino acids

KW - KB human oral epidermoid carcinoma cells

KW - L-Type amino acid transporter

UR - https://www.scopus.com/pages/publications/10744221316

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DO - 10.1016/j.canlet.2003.10.009

M3 - Article

C2 - 15036654

AN - SCOPUS:10744221316

SN - 0304-3835

VL - 205

SP - 215

EP - 226

JO - Cancer Letters

JF - Cancer Letters

IS - 2

ER -

Expression and functional characterization of the system L amino acid transporter in KB human oral epidermoid carcinoma cells

Abstract

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