TY - JOUR
T1 - Expression cloning and intracellular localization of a human ZF5 homologue
AU - Sugiura, Kazumitsu
AU - Muro, Yoshinao
AU - Nagai, Yasuo
AU - Kamimoto, Takahiro
AU - Wakabayashi, Takashi
AU - Ohashi, Masaru
AU - Hagiwara, Masatoshi
N1 - Funding Information:
We thank Hiroshi Onogi and Jun Koizumi for the DNA sequencing. This work was supported by grants-in-aid from the Ministry of Education, Science and Culture of Japan.
PY - 1997/5/2
Y1 - 1997/5/2
N2 - We isolated a cDNA encoding a human homologue of ZF5 (hZF5), which has five Kruppel-like C2H2 type zinc fingers at carboxyl terminus and the BTB/POZ (poxvirus and zinc finger) at the amino terminus, using autoimmune sera from a patient with overlap syndrome (dermatomyositis and scleroderma). Sequencing of the entire cDNA revealed an open reading frame (ORF) of 1349 bp with a deduced protein sequence of 449 amino acid residues and a calculated molecular weight of 51.3 kDa. The deduced amino acid sequence of hZF5 is highly homologous to mouse ZF5 (99.3% identity). Immunofluorescence studies revealed that HA-tagged hZF5 transiently expressed in COS-7 cells showed the nuclear dot pattern in the BTB/POZ domain-dependent manner.
AB - We isolated a cDNA encoding a human homologue of ZF5 (hZF5), which has five Kruppel-like C2H2 type zinc fingers at carboxyl terminus and the BTB/POZ (poxvirus and zinc finger) at the amino terminus, using autoimmune sera from a patient with overlap syndrome (dermatomyositis and scleroderma). Sequencing of the entire cDNA revealed an open reading frame (ORF) of 1349 bp with a deduced protein sequence of 449 amino acid residues and a calculated molecular weight of 51.3 kDa. The deduced amino acid sequence of hZF5 is highly homologous to mouse ZF5 (99.3% identity). Immunofluorescence studies revealed that HA-tagged hZF5 transiently expressed in COS-7 cells showed the nuclear dot pattern in the BTB/POZ domain-dependent manner.
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U2 - 10.1016/S0167-4781(97)00045-6
DO - 10.1016/S0167-4781(97)00045-6
M3 - Article
C2 - 9177479
AN - SCOPUS:0030949758
SN - 0167-4781
VL - 1352
SP - 23
EP - 26
JO - Biochimica et Biophysica Acta - Gene Structure and Expression
JF - Biochimica et Biophysica Acta - Gene Structure and Expression
IS - 1
ER -