Expression cloning and intracellular localization of a human ZF5 homologue

Kazumitsu Sugiura, Yoshinao Muro, Yasuo Nagai, Takahiro Kamimoto, Takashi Wakabayashi, Masaru Ohashi, Masatoshi Hagiwara

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

We isolated a cDNA encoding a human homologue of ZF5 (hZF5), which has five Kruppel-like C2H2 type zinc fingers at carboxyl terminus and the BTB/POZ (poxvirus and zinc finger) at the amino terminus, using autoimmune sera from a patient with overlap syndrome (dermatomyositis and scleroderma). Sequencing of the entire cDNA revealed an open reading frame (ORF) of 1349 bp with a deduced protein sequence of 449 amino acid residues and a calculated molecular weight of 51.3 kDa. The deduced amino acid sequence of hZF5 is highly homologous to mouse ZF5 (99.3% identity). Immunofluorescence studies revealed that HA-tagged hZF5 transiently expressed in COS-7 cells showed the nuclear dot pattern in the BTB/POZ domain-dependent manner.

Original languageEnglish
Pages (from-to)23-26
Number of pages4
JournalBiochimica et Biophysica Acta - Gene Structure and Expression
Volume1352
Issue number1
DOIs
Publication statusPublished - 02-05-1997
Externally publishedYes

Fingerprint

Cloning
Zinc
Organism Cloning
Complementary DNA
Poxviridae
Amino Acids
Amino Acid Sequence Homology
Dermatomyositis
COS Cells
Zinc Fingers
Open Reading Frames
Fluorescent Antibody Technique
Amino Acid Sequence
Molecular Weight
Molecular weight
Serum
Proteins
BTB-POZ Domain
CYS2-HIS2 Zinc Fingers

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Genetics
  • Structural Biology
  • Biophysics

Cite this

Sugiura, Kazumitsu ; Muro, Yoshinao ; Nagai, Yasuo ; Kamimoto, Takahiro ; Wakabayashi, Takashi ; Ohashi, Masaru ; Hagiwara, Masatoshi. / Expression cloning and intracellular localization of a human ZF5 homologue. In: Biochimica et Biophysica Acta - Gene Structure and Expression. 1997 ; Vol. 1352, No. 1. pp. 23-26.
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Expression cloning and intracellular localization of a human ZF5 homologue. / Sugiura, Kazumitsu; Muro, Yoshinao; Nagai, Yasuo; Kamimoto, Takahiro; Wakabayashi, Takashi; Ohashi, Masaru; Hagiwara, Masatoshi.

In: Biochimica et Biophysica Acta - Gene Structure and Expression, Vol. 1352, No. 1, 02.05.1997, p. 23-26.

Research output: Contribution to journalArticle

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AU - Sugiura, Kazumitsu

AU - Muro, Yoshinao

AU - Nagai, Yasuo

AU - Kamimoto, Takahiro

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AU - Ohashi, Masaru

AU - Hagiwara, Masatoshi

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