Expression cloning and intracellular localization of a human ZF5 homologue

Kazumitsu Sugiura, Yoshinao Muro, Yasuo Nagai, Takahiro Kamimoto, Takashi Wakabayashi, Masaru Ohashi, Masatoshi Hagiwara

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

We isolated a cDNA encoding a human homologue of ZF5 (hZF5), which has five Kruppel-like C2H2 type zinc fingers at carboxyl terminus and the BTB/POZ (poxvirus and zinc finger) at the amino terminus, using autoimmune sera from a patient with overlap syndrome (dermatomyositis and scleroderma). Sequencing of the entire cDNA revealed an open reading frame (ORF) of 1349 bp with a deduced protein sequence of 449 amino acid residues and a calculated molecular weight of 51.3 kDa. The deduced amino acid sequence of hZF5 is highly homologous to mouse ZF5 (99.3% identity). Immunofluorescence studies revealed that HA-tagged hZF5 transiently expressed in COS-7 cells showed the nuclear dot pattern in the BTB/POZ domain-dependent manner.

Original languageEnglish
Pages (from-to)23-26
Number of pages4
JournalBiochimica et Biophysica Acta - Gene Structure and Expression
Volume1352
Issue number1
DOIs
Publication statusPublished - 02-05-1997
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Structural Biology
  • Biophysics
  • Biochemistry
  • Genetics

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