Expression cloning and intracellular localization of a human ZF5 homologue

Kazumitsu Sugiura; Yoshinao Muro; Yasuo Nagai; Takahiro Kamimoto; Takashi Wakabayashi; Masaru Ohashi; Masatoshi Hagiwara

doi:10.1016/S0167-4781(97)00045-6

Expression cloning and intracellular localization of a human ZF5 homologue

Kazumitsu Sugiura
, Yoshinao Muro
, Yasuo Nagai
, Takahiro Kamimoto
, Takashi Wakabayashi
, Masaru Ohashi
, Masatoshi Hagiwara

Research output: Contribution to journal › Article › peer-review

19 Citations (Scopus)

Abstract

We isolated a cDNA encoding a human homologue of ZF5 (hZF5), which has five Kruppel-like C2H2 type zinc fingers at carboxyl terminus and the BTB/POZ (poxvirus and zinc finger) at the amino terminus, using autoimmune sera from a patient with overlap syndrome (dermatomyositis and scleroderma). Sequencing of the entire cDNA revealed an open reading frame (ORF) of 1349 bp with a deduced protein sequence of 449 amino acid residues and a calculated molecular weight of 51.3 kDa. The deduced amino acid sequence of hZF5 is highly homologous to mouse ZF5 (99.3% identity). Immunofluorescence studies revealed that HA-tagged hZF5 transiently expressed in COS-7 cells showed the nuclear dot pattern in the BTB/POZ domain-dependent manner.

Original language	English
Pages (from-to)	23-26
Number of pages	4
Journal	Biochimica et Biophysica Acta - Gene Structure and Expression
Volume	1352
Issue number	1
DOIs	https://doi.org/10.1016/S0167-4781(97)00045-6
Publication status	Published - 02-05-1997
Externally published	Yes

All Science Journal Classification (ASJC) codes

Structural Biology
Biophysics
Biochemistry
Genetics

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10.1016/S0167-4781(97)00045-6

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title = "Expression cloning and intracellular localization of a human ZF5 homologue",

abstract = "We isolated a cDNA encoding a human homologue of ZF5 (hZF5), which has five Kruppel-like C2H2 type zinc fingers at carboxyl terminus and the BTB/POZ (poxvirus and zinc finger) at the amino terminus, using autoimmune sera from a patient with overlap syndrome (dermatomyositis and scleroderma). Sequencing of the entire cDNA revealed an open reading frame (ORF) of 1349 bp with a deduced protein sequence of 449 amino acid residues and a calculated molecular weight of 51.3 kDa. The deduced amino acid sequence of hZF5 is highly homologous to mouse ZF5 (99.3\% identity). Immunofluorescence studies revealed that HA-tagged hZF5 transiently expressed in COS-7 cells showed the nuclear dot pattern in the BTB/POZ domain-dependent manner.",

keywords = "BTB/POZ domain, ZF5, nuclear localization, zinc finger",

author = "Kazumitsu Sugiura and Yoshinao Muro and Yasuo Nagai and Takahiro Kamimoto and Takashi Wakabayashi and Masaru Ohashi and Masatoshi Hagiwara",

year = "1997",

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doi = "10.1016/S0167-4781(97)00045-6",

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TY - JOUR

T1 - Expression cloning and intracellular localization of a human ZF5 homologue

AU - Sugiura, Kazumitsu

AU - Muro, Yoshinao

AU - Nagai, Yasuo

AU - Kamimoto, Takahiro

AU - Wakabayashi, Takashi

AU - Ohashi, Masaru

AU - Hagiwara, Masatoshi

PY - 1997/5/2

Y1 - 1997/5/2

N2 - We isolated a cDNA encoding a human homologue of ZF5 (hZF5), which has five Kruppel-like C2H2 type zinc fingers at carboxyl terminus and the BTB/POZ (poxvirus and zinc finger) at the amino terminus, using autoimmune sera from a patient with overlap syndrome (dermatomyositis and scleroderma). Sequencing of the entire cDNA revealed an open reading frame (ORF) of 1349 bp with a deduced protein sequence of 449 amino acid residues and a calculated molecular weight of 51.3 kDa. The deduced amino acid sequence of hZF5 is highly homologous to mouse ZF5 (99.3% identity). Immunofluorescence studies revealed that HA-tagged hZF5 transiently expressed in COS-7 cells showed the nuclear dot pattern in the BTB/POZ domain-dependent manner.

AB - We isolated a cDNA encoding a human homologue of ZF5 (hZF5), which has five Kruppel-like C2H2 type zinc fingers at carboxyl terminus and the BTB/POZ (poxvirus and zinc finger) at the amino terminus, using autoimmune sera from a patient with overlap syndrome (dermatomyositis and scleroderma). Sequencing of the entire cDNA revealed an open reading frame (ORF) of 1349 bp with a deduced protein sequence of 449 amino acid residues and a calculated molecular weight of 51.3 kDa. The deduced amino acid sequence of hZF5 is highly homologous to mouse ZF5 (99.3% identity). Immunofluorescence studies revealed that HA-tagged hZF5 transiently expressed in COS-7 cells showed the nuclear dot pattern in the BTB/POZ domain-dependent manner.

KW - BTB/POZ domain

KW - ZF5

KW - nuclear localization

KW - zinc finger

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UR - https://www.scopus.com/pages/publications/0030949758#tab=citedBy

U2 - 10.1016/S0167-4781(97)00045-6

DO - 10.1016/S0167-4781(97)00045-6

M3 - Article

C2 - 9177479

AN - SCOPUS:0030949758

SN - 0167-4781

VL - 1352

SP - 23

EP - 26

JO - Biochimica et Biophysica Acta - Gene Structure and Expression

JF - Biochimica et Biophysica Acta - Gene Structure and Expression

IS - 1

ER -

Expression cloning and intracellular localization of a human ZF5 homologue

Abstract

All Science Journal Classification (ASJC) codes

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