Expression cloning of oligomerization-activated genes with cell-proliferating potency by pseudotype retrovirus vector

Akihiro Abe, Nobuhiko Emi, Tadaharu Kanie, Shizuka Imagama, Yoshie Kuno, Masahide Takahashi, Hidehiko Saito, Tomoki Naoe

Research output: Contribution to journalArticlepeer-review

15 Citations (Scopus)

Abstract

We developed a method of clone proliferation promoting fusion genes whose proteins were activated by protein oligomerization through the helix-loop-helix region (PNT domain) of TEL. We inserted a cDNA library downstream of the PNT domain with a retrovirus vector. The resulting retrovirus infected cytokine-dependent 32D cells and cells with cytokine-independent growth were analyzed for the inserted cDNA. We cloned 25 independent fusion genes including seven kinds of partner genes. Six of the seven were a fusion of TEL with protein tyrosine kinase, LYN, HCK, FGR, SYK, FLT3, and TYK2. A serine/threonine kinase, ARAF1, was also found to fuse with TEL. These kinase fusion proteins included kinase domains with proper reading frames. These fusions may be a useful model for clarifying the downstream signal transduction of constitutive active kinase and this expression cloning method may provide a new tool with which to study cell proliferation signalling.

Original languageEnglish
Pages (from-to)920-926
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume320
Issue number3
DOIs
Publication statusPublished - 30-07-2004
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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