Expression level of enzymes related to in situ estrogen synthesis and clinicopathological parameters in breast cancer patients

Masayo Suzuki, Hiroyuki Ishida, Yukimasa Shiotsu, Taisuke Nakata, Shiro Akinaga, Shigemitsu Takashima, Toshiaki Utsumi, Toshiaki Saeki, Nobuhiro Harada

Research output: Contribution to journalArticlepeer-review

27 Citations (Scopus)

Abstract

In order to evaluate the importance of estrogen production in tumor and surrounding tissues, we measured mRNA expression levels of 5 enzymes participating to estrogen synthesis in situ and 4 breast cancer-related proteins in 27 pairs of tumor and non-malignant tissues. Steroid sulfatase (STS) mRNA was more frequently detected in tumor tissues rather than in their non-malignant counterparts. Estrogen sulfotransferase (EST) was constantly expressed with high level not only in tumor tissues but also in their surrounding non-malignant counterparts. In contrast, mRNA expression levels of aromatase, and 17β-hydroxysteroid dehydrogenase type I and II were relatively low and detected only in small proportion of the patients. We also measured the mRNA expression levels of the same nine genes in tumor tissues of 197 breast cancer patients, and analyzed relationship between the mRNA expression level and the clinicopathological parameters. The mRNA expression levels of STS, aromatase and erbB2 in tumor tissues increased as breast cancer progressed. The tumoral mRNA expression levels of STS, estrogen receptor β, and erbB2 in patients with recurrence were higher than those in patients without recurrence. Upregulation of STS expression plays an important role in tumor progression of human breast cancer and is considered to be responsible for estrogen production in tumor and surrounding tissues.

Original languageEnglish
Pages (from-to)195-201
Number of pages7
JournalJournal of Steroid Biochemistry and Molecular Biology
Volume113
Issue number3-5
DOIs
Publication statusPublished - 01-02-2009

All Science Journal Classification (ASJC) codes

  • Endocrinology, Diabetes and Metabolism
  • Biochemistry
  • Molecular Medicine
  • Molecular Biology
  • Endocrinology
  • Clinical Biochemistry
  • Cell Biology

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