Expression of a TGF-β1 inducible gene, TSC-36, causes growth inhibition in human lung cancer cell lines

Kenya Sumitomo, Akira Kurisaki, Norio Yamakawa, Kunihiro Tsuchida, Eiji Shimizu, Saburo Sone, Hiromu Sugino

Research output: Contribution to journalArticlepeer-review

64 Citations (Scopus)

Abstract

TSC-36 (TGF-β1-stimulated clone 36) is a TGF-β1 inducible gene whose product is an extracellular glycoprotein that contains a single follistatin module. TSC-36 is highly expressed in the lung, but its physiological function is unknown. In an attempt to elucidate it, we investigated the effect of TSC-36 on proliferation of human lung cancer cell lines. We found a correlation between expression of TSC-36 and cell growth: TSC-36 mRNA was not detected in cells derived from small cell lung cancer (SCLC) cells, a highly aggressive neoplasm, but was detected in some non-small cell lung cancer (NSCLC) cells, a moderately aggressive neoplasm. This suggested an antiproliferative function for TSC-36. To address this question, NSCLC PC-14 cells, which express very low level of TSC-36 protein, were transfected with TSC-36 cDNA and the proliferative capacity of stable transfectants was determined by measuring the doubling time, colony forming activity in soft agar and the level of incorporation of 3H-thymidine into DNA. Under normal culture conditions, the transfected cells showed a longer doubling time, lower plating efficiency and lower rate of DNA synthesis than the parental cells and the control neo transfectant cells. These findings suggested that expression of TSC-36 caused growth inhibition in human lung cancer cells. (C) 2000 Elsevier Science Ireland Ltd.

Original languageEnglish
Pages (from-to)37-46
Number of pages10
JournalCancer Letters
Volume155
Issue number1
DOIs
Publication statusPublished - 03-07-2000

All Science Journal Classification (ASJC) codes

  • Oncology
  • Cancer Research

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