TY - JOUR
T1 - Expression of a TGF-β1 inducible gene, TSC-36, causes growth inhibition in human lung cancer cell lines
AU - Sumitomo, Kenya
AU - Kurisaki, Akira
AU - Yamakawa, Norio
AU - Tsuchida, Kunihiro
AU - Shimizu, Eiji
AU - Sone, Saburo
AU - Sugino, Hiromu
N1 - Funding Information:
We thank Dr K. Nose for providing mouse TSC-36 cDNA, Drs K. Tani, S. Yano, P.P. Ranjan and K. Sugino for helpful discussion and advise, and Dr G.R. Bousfield for critically reading the manuscript. This work was supported in part by grants from the Ministry of Education, Science, Sports and Culture, Japan (H.S., S.S.), the Takeda Science Foundation (K.T.) and Uehara Memorial Foundation (K.T.).
PY - 2000/7/3
Y1 - 2000/7/3
N2 - TSC-36 (TGF-β1-stimulated clone 36) is a TGF-β1 inducible gene whose product is an extracellular glycoprotein that contains a single follistatin module. TSC-36 is highly expressed in the lung, but its physiological function is unknown. In an attempt to elucidate it, we investigated the effect of TSC-36 on proliferation of human lung cancer cell lines. We found a correlation between expression of TSC-36 and cell growth: TSC-36 mRNA was not detected in cells derived from small cell lung cancer (SCLC) cells, a highly aggressive neoplasm, but was detected in some non-small cell lung cancer (NSCLC) cells, a moderately aggressive neoplasm. This suggested an antiproliferative function for TSC-36. To address this question, NSCLC PC-14 cells, which express very low level of TSC-36 protein, were transfected with TSC-36 cDNA and the proliferative capacity of stable transfectants was determined by measuring the doubling time, colony forming activity in soft agar and the level of incorporation of 3H-thymidine into DNA. Under normal culture conditions, the transfected cells showed a longer doubling time, lower plating efficiency and lower rate of DNA synthesis than the parental cells and the control neo transfectant cells. These findings suggested that expression of TSC-36 caused growth inhibition in human lung cancer cells. (C) 2000 Elsevier Science Ireland Ltd.
AB - TSC-36 (TGF-β1-stimulated clone 36) is a TGF-β1 inducible gene whose product is an extracellular glycoprotein that contains a single follistatin module. TSC-36 is highly expressed in the lung, but its physiological function is unknown. In an attempt to elucidate it, we investigated the effect of TSC-36 on proliferation of human lung cancer cell lines. We found a correlation between expression of TSC-36 and cell growth: TSC-36 mRNA was not detected in cells derived from small cell lung cancer (SCLC) cells, a highly aggressive neoplasm, but was detected in some non-small cell lung cancer (NSCLC) cells, a moderately aggressive neoplasm. This suggested an antiproliferative function for TSC-36. To address this question, NSCLC PC-14 cells, which express very low level of TSC-36 protein, were transfected with TSC-36 cDNA and the proliferative capacity of stable transfectants was determined by measuring the doubling time, colony forming activity in soft agar and the level of incorporation of 3H-thymidine into DNA. Under normal culture conditions, the transfected cells showed a longer doubling time, lower plating efficiency and lower rate of DNA synthesis than the parental cells and the control neo transfectant cells. These findings suggested that expression of TSC-36 caused growth inhibition in human lung cancer cells. (C) 2000 Elsevier Science Ireland Ltd.
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U2 - 10.1016/S0304-3835(00)00407-9
DO - 10.1016/S0304-3835(00)00407-9
M3 - Article
C2 - 10814877
AN - SCOPUS:0034600570
SN - 0304-3835
VL - 155
SP - 37
EP - 46
JO - Cancer Letters
JF - Cancer Letters
IS - 1
ER -
