Expression of CD47/integrin-associated protein induces death of cultured cerebral cortical neurons

Hisatsugu Koshimizu, Toshiyuki Araki, Satomi Takai, Daisaku Yokomaku, Yasuyuki Ishikawa, Misae Kubota, Shin Ichiro Sano, Hiroshi Hatanaka, Masashi Yamada

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

The death and survival of neuronal cells are regulated by various signaling pathways during development of the brain and in neuronal diseases. Previously, we demonstrated that the neuronal adhesion molecule brain immunoglobulin-like molecule with tyrosine-based activation motifs/SHP substrate 1 (BIT/SHPS-1) is involved in brain-derived neurotrophic factor (BDNF)-promoted neuronal cell survival. Here, we report the apoptosis-inducing effect of CD47/integrin-associated protein (IAP), the heterophilic binding partner of BIT/SHPS-1, on neuronal cells. We generated a recombinant adenovirus vector expressing a neuronal form of CD47/IAP, and found that the expression of CD47/IAP by infection with CD47/IAP adeno- virus induced the death of cultured cerebral cortical neurons. The numbers of TdT-mediated biotin-dUTP nick-end labelling (TUNEL)-positive neurons and of cells displaying apoptotic nuclei increased by expression of CD47/IAP. Neuronal cell death was prevented by the addition of the broad-spectrum caspase inhibitor Z-VAD-fmk. Furthermore, we observed that co-expression of CD47/IAP with BIT/SHPS-1 enhanced neuronal cell death, and that BDNF prevented it. These results suggest that CD47/IAP is involved in a novel pathway which regulates caspase-dependent apoptosis of cultured cerebral cortical neurons. CD47/IAP-induced death of cultured cortical neurons may be regulated by the interaction of CD47/IAP with BIT/SHPS-1 and by BDNF.

Original languageEnglish
Pages (from-to)249-257
Number of pages9
JournalJournal of Neurochemistry
Volume82
Issue number2
DOIs
Publication statusPublished - 25-07-2002

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Integrins
Neurons
Proteins
Brain-Derived Neurotrophic Factor
Chemical activation
Cell death
Substrates
Brain
Cell Survival
Cell Death
Cells
Apoptosis
Dependovirus
Molecules
Caspase Inhibitors
Biotin
Caspases
Viruses
Protein Binding
Adenoviridae

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Cellular and Molecular Neuroscience

Cite this

Koshimizu, Hisatsugu ; Araki, Toshiyuki ; Takai, Satomi ; Yokomaku, Daisaku ; Ishikawa, Yasuyuki ; Kubota, Misae ; Sano, Shin Ichiro ; Hatanaka, Hiroshi ; Yamada, Masashi. / Expression of CD47/integrin-associated protein induces death of cultured cerebral cortical neurons. In: Journal of Neurochemistry. 2002 ; Vol. 82, No. 2. pp. 249-257.
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Koshimizu, H, Araki, T, Takai, S, Yokomaku, D, Ishikawa, Y, Kubota, M, Sano, SI, Hatanaka, H & Yamada, M 2002, 'Expression of CD47/integrin-associated protein induces death of cultured cerebral cortical neurons', Journal of Neurochemistry, vol. 82, no. 2, pp. 249-257. https://doi.org/10.1046/j.1471-4159.2002.00965.x

Expression of CD47/integrin-associated protein induces death of cultured cerebral cortical neurons. / Koshimizu, Hisatsugu; Araki, Toshiyuki; Takai, Satomi; Yokomaku, Daisaku; Ishikawa, Yasuyuki; Kubota, Misae; Sano, Shin Ichiro; Hatanaka, Hiroshi; Yamada, Masashi.

In: Journal of Neurochemistry, Vol. 82, No. 2, 25.07.2002, p. 249-257.

Research output: Contribution to journalArticle

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T1 - Expression of CD47/integrin-associated protein induces death of cultured cerebral cortical neurons

AU - Koshimizu, Hisatsugu

AU - Araki, Toshiyuki

AU - Takai, Satomi

AU - Yokomaku, Daisaku

AU - Ishikawa, Yasuyuki

AU - Kubota, Misae

AU - Sano, Shin Ichiro

AU - Hatanaka, Hiroshi

AU - Yamada, Masashi

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