Expression of cystatin C prevents oxidative stress-induced death in PC12 cells

Keiji Nishiyama, Akio Konishi, Chika Nishio, Kiyomi Araki-Yoshida, Hiroshi Hatanaka, Masami Kojima, Yoshihiro Ohmiya, Masashi Yamada, Hisatsugu Koshimizu

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32 Citations (Scopus)


Cystatin C, an inhibitor of cysteine proteinases, is suggested to be involved in oxidative stress-induced apoptosis of cultured CNS neurons and various neuronal diseases in vivo; however, little is known about its mechanism of action. To address the role cystatin C plays in oxidative stress-induced neuronal cell death, we established PC12 cell lines that stably expressed rat cystatin C. These cystatin C-expressing PC12 cells showed remarkable resistance to high (50%) oxygen atmosphere. This resistance correlate with expression levels of cystatin C, demonstrating that cystatin C has a protective effect on high oxygen-induced cell death. In contrast, in a normal (20%) oxygen atmosphere neither control nor cystatin C-expressing PC12 cells showed a significant change in the number of living cells, indicating that cystatin C does not play an important role in the regulation of cellular proliferation. Furthermore, the cystatin C-expressing cell line also resisted other oxidative stresses, including glutamate- and 13-l-hydroperoxylinoleic acid (LOOH)-induced cell death. These results demonstrate that cystatin C has protective effects against various oxidative stresses that induce cell death.

Original languageEnglish
Pages (from-to)94-99
Number of pages6
JournalBrain Research Bulletin
Issue number1-2
Publication statusPublished - 30-09-2005
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)


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