Expression of cystatin C prevents oxidative stress-induced death in PC12 cells

Keiji Nishiyama, Akio Konishi, Chika Nishio, Kiyomi Araki-Yoshida, Hiroshi Hatanaka, Masami Kojima, Yoshihiro Ohmiya, Masashi Yamada, Hisatsugu Koshimizu

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

Cystatin C, an inhibitor of cysteine proteinases, is suggested to be involved in oxidative stress-induced apoptosis of cultured CNS neurons and various neuronal diseases in vivo; however, little is known about its mechanism of action. To address the role cystatin C plays in oxidative stress-induced neuronal cell death, we established PC12 cell lines that stably expressed rat cystatin C. These cystatin C-expressing PC12 cells showed remarkable resistance to high (50%) oxygen atmosphere. This resistance correlate with expression levels of cystatin C, demonstrating that cystatin C has a protective effect on high oxygen-induced cell death. In contrast, in a normal (20%) oxygen atmosphere neither control nor cystatin C-expressing PC12 cells showed a significant change in the number of living cells, indicating that cystatin C does not play an important role in the regulation of cellular proliferation. Furthermore, the cystatin C-expressing cell line also resisted other oxidative stresses, including glutamate- and 13-l-hydroperoxylinoleic acid (LOOH)-induced cell death. These results demonstrate that cystatin C has protective effects against various oxidative stresses that induce cell death.

Original languageEnglish
Pages (from-to)94-99
Number of pages6
JournalBrain Research Bulletin
Volume67
Issue number1-2
DOIs
Publication statusPublished - 30-09-2005

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Cystatin C
PC12 Cells
Oxidative Stress
Cell Death
Oxygen
Atmosphere
Cysteine Proteinase Inhibitors
Cell Line
Glutamic Acid
Cell Count
Cell Proliferation
Apoptosis
Neurons

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)

Cite this

Nishiyama, Keiji ; Konishi, Akio ; Nishio, Chika ; Araki-Yoshida, Kiyomi ; Hatanaka, Hiroshi ; Kojima, Masami ; Ohmiya, Yoshihiro ; Yamada, Masashi ; Koshimizu, Hisatsugu. / Expression of cystatin C prevents oxidative stress-induced death in PC12 cells. In: Brain Research Bulletin. 2005 ; Vol. 67, No. 1-2. pp. 94-99.
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Nishiyama, K, Konishi, A, Nishio, C, Araki-Yoshida, K, Hatanaka, H, Kojima, M, Ohmiya, Y, Yamada, M & Koshimizu, H 2005, 'Expression of cystatin C prevents oxidative stress-induced death in PC12 cells', Brain Research Bulletin, vol. 67, no. 1-2, pp. 94-99. https://doi.org/10.1016/j.brainresbull.2005.05.020

Expression of cystatin C prevents oxidative stress-induced death in PC12 cells. / Nishiyama, Keiji; Konishi, Akio; Nishio, Chika; Araki-Yoshida, Kiyomi; Hatanaka, Hiroshi; Kojima, Masami; Ohmiya, Yoshihiro; Yamada, Masashi; Koshimizu, Hisatsugu.

In: Brain Research Bulletin, Vol. 67, No. 1-2, 30.09.2005, p. 94-99.

Research output: Contribution to journalArticle

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AU - Konishi, Akio

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AU - Hatanaka, Hiroshi

AU - Kojima, Masami

AU - Ohmiya, Yoshihiro

AU - Yamada, Masashi

AU - Koshimizu, Hisatsugu

PY - 2005/9/30

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N2 - Cystatin C, an inhibitor of cysteine proteinases, is suggested to be involved in oxidative stress-induced apoptosis of cultured CNS neurons and various neuronal diseases in vivo; however, little is known about its mechanism of action. To address the role cystatin C plays in oxidative stress-induced neuronal cell death, we established PC12 cell lines that stably expressed rat cystatin C. These cystatin C-expressing PC12 cells showed remarkable resistance to high (50%) oxygen atmosphere. This resistance correlate with expression levels of cystatin C, demonstrating that cystatin C has a protective effect on high oxygen-induced cell death. In contrast, in a normal (20%) oxygen atmosphere neither control nor cystatin C-expressing PC12 cells showed a significant change in the number of living cells, indicating that cystatin C does not play an important role in the regulation of cellular proliferation. Furthermore, the cystatin C-expressing cell line also resisted other oxidative stresses, including glutamate- and 13-l-hydroperoxylinoleic acid (LOOH)-induced cell death. These results demonstrate that cystatin C has protective effects against various oxidative stresses that induce cell death.

AB - Cystatin C, an inhibitor of cysteine proteinases, is suggested to be involved in oxidative stress-induced apoptosis of cultured CNS neurons and various neuronal diseases in vivo; however, little is known about its mechanism of action. To address the role cystatin C plays in oxidative stress-induced neuronal cell death, we established PC12 cell lines that stably expressed rat cystatin C. These cystatin C-expressing PC12 cells showed remarkable resistance to high (50%) oxygen atmosphere. This resistance correlate with expression levels of cystatin C, demonstrating that cystatin C has a protective effect on high oxygen-induced cell death. In contrast, in a normal (20%) oxygen atmosphere neither control nor cystatin C-expressing PC12 cells showed a significant change in the number of living cells, indicating that cystatin C does not play an important role in the regulation of cellular proliferation. Furthermore, the cystatin C-expressing cell line also resisted other oxidative stresses, including glutamate- and 13-l-hydroperoxylinoleic acid (LOOH)-induced cell death. These results demonstrate that cystatin C has protective effects against various oxidative stresses that induce cell death.

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Nishiyama K, Konishi A, Nishio C, Araki-Yoshida K, Hatanaka H, Kojima M et al. Expression of cystatin C prevents oxidative stress-induced death in PC12 cells. Brain Research Bulletin. 2005 Sep 30;67(1-2):94-99. https://doi.org/10.1016/j.brainresbull.2005.05.020