Expression of human scavenger receptor class B type I in cultured human monocyte-derived macrophages and atherosclerotic lesions

Ken Ichi Hirano, Shizuya Yamashita, Yumiko Nakagawa, Takeshi Ohya, Fumihiko Matsuura, Kosuke Tsukamoto, Yoshihisa Okamoto, Akifumi Matsuyama, Kengo Matsumoto, Jun Ichiro Miyagawa, Yuji Matsuzawa

Research output: Contribution to journalArticle

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Abstract

The scavenger receptor class B type I (SR-BI) and its human homologue CLA-1 (CD36 and LIMPII Analogous-1) have recently been identified to bind HDL and mediate the selective uptake of HDL lipids. Tissue distribution of both murine and human receptors is quite similar, in that they are expressed abundantly in liver and steroidogenic tissues. However, expression and function of the human SR-BI (hSR-BI), in the periphery of reverse cholesterol transport such as macrophages, are still unclear. In the present study, we have raised two different kinds of anti-hSR-BI polypeptide antibodies (Abs): one against the extracellular domain and the other against the intracellular domain. We have investigated the expression of hSR-BI mRNA and immunoreactive mass in freshly isolated cultured human monocyte-derived macrophages (hMφ) and in atherosclerotic lesions. Contrary to the earlier report, hSR-BI mRNA was expressed in cultured hMφ and markedly upregulated with differentiation, determined by Northern blot and reverse transcriptase-based polymerase chain reaction analyses. The mRNA expression pattern during differentiation of hMφ was very similar to those of SR class A and another member of SR class B, CD36. Protein expression was confirmed by Western blot analyses with the above Abs to show a major 83-kDa band. Modified lipoproteins such as oxidized LDL and acetylated LDL induced a 5-fold increase in mRNA and protein expression of hSR-BI. Confocal immunofluorescence microscopy demonstrated that hSR-BI immunoreactive mass was detectable as a heterogeneous, punctate staining pattern. Furthermore, immunohistochemical analysis showed that immunoreactive mass of hSR-BI was detected in foam cells in human aortic atherosclerotic lesions and that there was no significant difference of staining patterns between the two Abs. This study clearly demonstrates that hSR-BI is expressed in the lipid-laden macrophages in human atherosclerotic lesions, suggesting that it is very important to know its function and regulation in hMφ to understand the biological utility of this molecule.

Original languageEnglish
Pages (from-to)108-116
Number of pages9
JournalCirculation Research
Volume85
Issue number1
DOIs
Publication statusPublished - 09-07-1999

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CD36 Antigens
Macrophages
Messenger RNA
Antibodies
Staining and Labeling
Lipids
Foam Cells
Tissue Distribution
Reverse Transcriptase Polymerase Chain Reaction
Fluorescence Microscopy
Confocal Microscopy
Northern Blotting
Lipoproteins

All Science Journal Classification (ASJC) codes

  • Physiology
  • Cardiology and Cardiovascular Medicine

Cite this

Hirano, K. I., Yamashita, S., Nakagawa, Y., Ohya, T., Matsuura, F., Tsukamoto, K., ... Matsuzawa, Y. (1999). Expression of human scavenger receptor class B type I in cultured human monocyte-derived macrophages and atherosclerotic lesions. Circulation Research, 85(1), 108-116. https://doi.org/10.1161/01.RES.85.1.108
Hirano, Ken Ichi ; Yamashita, Shizuya ; Nakagawa, Yumiko ; Ohya, Takeshi ; Matsuura, Fumihiko ; Tsukamoto, Kosuke ; Okamoto, Yoshihisa ; Matsuyama, Akifumi ; Matsumoto, Kengo ; Miyagawa, Jun Ichiro ; Matsuzawa, Yuji. / Expression of human scavenger receptor class B type I in cultured human monocyte-derived macrophages and atherosclerotic lesions. In: Circulation Research. 1999 ; Vol. 85, No. 1. pp. 108-116.
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abstract = "The scavenger receptor class B type I (SR-BI) and its human homologue CLA-1 (CD36 and LIMPII Analogous-1) have recently been identified to bind HDL and mediate the selective uptake of HDL lipids. Tissue distribution of both murine and human receptors is quite similar, in that they are expressed abundantly in liver and steroidogenic tissues. However, expression and function of the human SR-BI (hSR-BI), in the periphery of reverse cholesterol transport such as macrophages, are still unclear. In the present study, we have raised two different kinds of anti-hSR-BI polypeptide antibodies (Abs): one against the extracellular domain and the other against the intracellular domain. We have investigated the expression of hSR-BI mRNA and immunoreactive mass in freshly isolated cultured human monocyte-derived macrophages (hMφ) and in atherosclerotic lesions. Contrary to the earlier report, hSR-BI mRNA was expressed in cultured hMφ and markedly upregulated with differentiation, determined by Northern blot and reverse transcriptase-based polymerase chain reaction analyses. The mRNA expression pattern during differentiation of hMφ was very similar to those of SR class A and another member of SR class B, CD36. Protein expression was confirmed by Western blot analyses with the above Abs to show a major 83-kDa band. Modified lipoproteins such as oxidized LDL and acetylated LDL induced a 5-fold increase in mRNA and protein expression of hSR-BI. Confocal immunofluorescence microscopy demonstrated that hSR-BI immunoreactive mass was detectable as a heterogeneous, punctate staining pattern. Furthermore, immunohistochemical analysis showed that immunoreactive mass of hSR-BI was detected in foam cells in human aortic atherosclerotic lesions and that there was no significant difference of staining patterns between the two Abs. This study clearly demonstrates that hSR-BI is expressed in the lipid-laden macrophages in human atherosclerotic lesions, suggesting that it is very important to know its function and regulation in hMφ to understand the biological utility of this molecule.",
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Hirano, KI, Yamashita, S, Nakagawa, Y, Ohya, T, Matsuura, F, Tsukamoto, K, Okamoto, Y, Matsuyama, A, Matsumoto, K, Miyagawa, JI & Matsuzawa, Y 1999, 'Expression of human scavenger receptor class B type I in cultured human monocyte-derived macrophages and atherosclerotic lesions', Circulation Research, vol. 85, no. 1, pp. 108-116. https://doi.org/10.1161/01.RES.85.1.108

Expression of human scavenger receptor class B type I in cultured human monocyte-derived macrophages and atherosclerotic lesions. / Hirano, Ken Ichi; Yamashita, Shizuya; Nakagawa, Yumiko; Ohya, Takeshi; Matsuura, Fumihiko; Tsukamoto, Kosuke; Okamoto, Yoshihisa; Matsuyama, Akifumi; Matsumoto, Kengo; Miyagawa, Jun Ichiro; Matsuzawa, Yuji.

In: Circulation Research, Vol. 85, No. 1, 09.07.1999, p. 108-116.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Expression of human scavenger receptor class B type I in cultured human monocyte-derived macrophages and atherosclerotic lesions

AU - Hirano, Ken Ichi

AU - Yamashita, Shizuya

AU - Nakagawa, Yumiko

AU - Ohya, Takeshi

AU - Matsuura, Fumihiko

AU - Tsukamoto, Kosuke

AU - Okamoto, Yoshihisa

AU - Matsuyama, Akifumi

AU - Matsumoto, Kengo

AU - Miyagawa, Jun Ichiro

AU - Matsuzawa, Yuji

PY - 1999/7/9

Y1 - 1999/7/9

N2 - The scavenger receptor class B type I (SR-BI) and its human homologue CLA-1 (CD36 and LIMPII Analogous-1) have recently been identified to bind HDL and mediate the selective uptake of HDL lipids. Tissue distribution of both murine and human receptors is quite similar, in that they are expressed abundantly in liver and steroidogenic tissues. However, expression and function of the human SR-BI (hSR-BI), in the periphery of reverse cholesterol transport such as macrophages, are still unclear. In the present study, we have raised two different kinds of anti-hSR-BI polypeptide antibodies (Abs): one against the extracellular domain and the other against the intracellular domain. We have investigated the expression of hSR-BI mRNA and immunoreactive mass in freshly isolated cultured human monocyte-derived macrophages (hMφ) and in atherosclerotic lesions. Contrary to the earlier report, hSR-BI mRNA was expressed in cultured hMφ and markedly upregulated with differentiation, determined by Northern blot and reverse transcriptase-based polymerase chain reaction analyses. The mRNA expression pattern during differentiation of hMφ was very similar to those of SR class A and another member of SR class B, CD36. Protein expression was confirmed by Western blot analyses with the above Abs to show a major 83-kDa band. Modified lipoproteins such as oxidized LDL and acetylated LDL induced a 5-fold increase in mRNA and protein expression of hSR-BI. Confocal immunofluorescence microscopy demonstrated that hSR-BI immunoreactive mass was detectable as a heterogeneous, punctate staining pattern. Furthermore, immunohistochemical analysis showed that immunoreactive mass of hSR-BI was detected in foam cells in human aortic atherosclerotic lesions and that there was no significant difference of staining patterns between the two Abs. This study clearly demonstrates that hSR-BI is expressed in the lipid-laden macrophages in human atherosclerotic lesions, suggesting that it is very important to know its function and regulation in hMφ to understand the biological utility of this molecule.

AB - The scavenger receptor class B type I (SR-BI) and its human homologue CLA-1 (CD36 and LIMPII Analogous-1) have recently been identified to bind HDL and mediate the selective uptake of HDL lipids. Tissue distribution of both murine and human receptors is quite similar, in that they are expressed abundantly in liver and steroidogenic tissues. However, expression and function of the human SR-BI (hSR-BI), in the periphery of reverse cholesterol transport such as macrophages, are still unclear. In the present study, we have raised two different kinds of anti-hSR-BI polypeptide antibodies (Abs): one against the extracellular domain and the other against the intracellular domain. We have investigated the expression of hSR-BI mRNA and immunoreactive mass in freshly isolated cultured human monocyte-derived macrophages (hMφ) and in atherosclerotic lesions. Contrary to the earlier report, hSR-BI mRNA was expressed in cultured hMφ and markedly upregulated with differentiation, determined by Northern blot and reverse transcriptase-based polymerase chain reaction analyses. The mRNA expression pattern during differentiation of hMφ was very similar to those of SR class A and another member of SR class B, CD36. Protein expression was confirmed by Western blot analyses with the above Abs to show a major 83-kDa band. Modified lipoproteins such as oxidized LDL and acetylated LDL induced a 5-fold increase in mRNA and protein expression of hSR-BI. Confocal immunofluorescence microscopy demonstrated that hSR-BI immunoreactive mass was detectable as a heterogeneous, punctate staining pattern. Furthermore, immunohistochemical analysis showed that immunoreactive mass of hSR-BI was detected in foam cells in human aortic atherosclerotic lesions and that there was no significant difference of staining patterns between the two Abs. This study clearly demonstrates that hSR-BI is expressed in the lipid-laden macrophages in human atherosclerotic lesions, suggesting that it is very important to know its function and regulation in hMφ to understand the biological utility of this molecule.

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