Expression of indoleamine 2,3-dioxygenase in leukemic cells indicates an unfavorable prognosis in acute myeloid leukemia patients with intermediate-risk cytogenetics

Kenji Fukuno, Takeshi Hara, Hisashi Tsurumi, Yuhei Shibata, Ryoko Mabuchi, Nobuhiko Nakamura, Junichi Kitagawa, Masahito Shimizu, Hiroyasu Ito, Kuniaki Saito, Hisataka Moriwaki

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22 Citations (Scopus)

Abstract

The immunomodulatory effects of indoleamine 2,3-dioxygenase (IDO) are ascribed to its ability to catalyze breakdown of the essential amino acid l-tryptophan. We applied reverse transcription-polymerase chain reaction (RT-PCR) to examine IDO mRNA expression in acute myeloid leukemia (AML) blasts, and investigated its clinical significance. We enrolled 62 patients with AML between April 2005 and March 2013. Bone marrow-derived mononuclear fractions were separated and extracted mRNA was amplified by PCR. RT-PCR showed that the bone marrow of 23 patients expressed IDO mRNA but not in 39. IDO mRNA expression did not significantly differ among cytogenetic risk profiles. The 3-year overall survival rates for patients with and without IDO mRNA expression were 39% and 74%, respectively (p < 0.005). The rates for patients with intermediate-risk cytogenetics with and without IDO mRNA expression were 16% and 70%, respectively (p < 0.005). The expression of IDO mRNA was associated with a poor prognosis of AML.

Original languageEnglish
Pages (from-to)1398-1405
Number of pages8
JournalLeukemia and Lymphoma
Volume56
Issue number5
DOIs
Publication statusPublished - 01-05-2015

All Science Journal Classification (ASJC) codes

  • Hematology
  • Oncology
  • Cancer Research

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