TY - JOUR
T1 - Expression of LY6D is induced at the surface of MCF10A cells by X-ray irradiation
AU - Kurosawa, Maiko
AU - Jeyasekharan, Anand D.
AU - Surmann, Eva Maria
AU - Hashimoto, Noboru
AU - Venkatraman, Vignesh
AU - Kurosawa, Gene
AU - Furukawa, Koichi
AU - Venkitaraman, Ashok R.
AU - Kurosawa, Yoshikazu
PY - 2012/12
Y1 - 2012/12
N2 - In order to identify membrane proteins whose expression is induced by X-ray irradiation, we developed an antibody (Ab)-directed strategy using a phage Ab library. X-Ray-irradiated cells were screened with a phage Ab library in the presence of a large excess of polyclonal Abs prepared against membrane proteins that are commonly present at the surface of both X-ray-irradiated and nonirradiated cells. After isolation of Ab that bound only to X-ray-irradiated cells, the antigen was identified using MS. Using this approach, we found that expression of LY6D is induced in MCF10A cells by X-ray irradiation. The induction of LY6D expression is triggered through a pathway regulated by ATM, CHK2 and p53. This method is a new Ab-directed proteomic strategy for analysis of membrane proteins, and is applicable to various biological phenomena in situations in which both target molecule-expressing cells and nonexpressing cells are available.
AB - In order to identify membrane proteins whose expression is induced by X-ray irradiation, we developed an antibody (Ab)-directed strategy using a phage Ab library. X-Ray-irradiated cells were screened with a phage Ab library in the presence of a large excess of polyclonal Abs prepared against membrane proteins that are commonly present at the surface of both X-ray-irradiated and nonirradiated cells. After isolation of Ab that bound only to X-ray-irradiated cells, the antigen was identified using MS. Using this approach, we found that expression of LY6D is induced in MCF10A cells by X-ray irradiation. The induction of LY6D expression is triggered through a pathway regulated by ATM, CHK2 and p53. This method is a new Ab-directed proteomic strategy for analysis of membrane proteins, and is applicable to various biological phenomena in situations in which both target molecule-expressing cells and nonexpressing cells are available.
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U2 - 10.1111/febs.12034
DO - 10.1111/febs.12034
M3 - Article
C2 - 23075424
AN - SCOPUS:84870523813
SN - 1742-464X
VL - 279
SP - 4479
EP - 4491
JO - FEBS Journal
JF - FEBS Journal
IS - 24
ER -