TY - JOUR
T1 - Expression of macrophage-derived chemokine (MDC)/CCL22 in human lung cancer
AU - Nakanishi, Toru
AU - Imaizumi, Kazuyoshi
AU - Hasegawa, Yoshinori
AU - Kawabe, Tsutomu
AU - Hashimoto, Naozumi
AU - Okamoto, Masakazu
AU - Shimokata, Kaoru
N1 - Funding Information:
Acknowledgements The authors thank Ms. Ayako Asai, Emi Kus-ama, Hiromi Isejima and Keiko Shimamoto for their continuous technical assistance. This work was supported in part by a Grant-in-Aid for the 21st Century COE Program ‘‘Integrated Molecular Medicine for Neuronal and Neoplastic Disorders’’ from the Ministry of Education, Culture, Sports, Science and Technology and a Grant-in-Aid for the Scientific Research from the Japan Society for the Promotion of Science.
PY - 2006/11
Y1 - 2006/11
N2 - Background: Ligands for CXCR3 chemokines [IFN-γ-inducible protein of 10 kD (IP-10/CXCL10), monokine induced by IFN-γ (Mig/CXCL9), IFN-inducible T cell α chemoattractant (I-TAC/CXCL11)] and those for CCR4 [macrophage-derived chemokine (MDC/CCL22), thymus- and activation-regulated chemokine (TARC/CCL17)] have been shown to play the central roles for T helper-cell recruitment into the tissues. To examine the role of these chemokines in tumor progression of lung cancer, we investigated their expression in human lung cancer tissues to determine the possible relationship between their expression and the prognosis of patients. Methods: Total RNA was prepared from lung cancer tissues of 40 patients (24 adenocarcinoma and 16 squamous cell carcinoma). We measured gene expression levels of chemokines (IP-10, Mig, I-TAC, MDC and TARC) by real-time quantitative RT-PCR. Results: Higher gene expression of MDC in lung cancer was significantly correlated with longer disease-free survival time and lower risk of recurrence after tumor resection. We could not find any significant relationship of IP-10, Mig, I-TAC and TARC gene expression with disease-free survival or lower risk of recurrence after surgery. Conclusions: These results suggest that increased gene expression of MDC in tumor tissues may be a predictive marker for improving the prognosis of lung cancer.
AB - Background: Ligands for CXCR3 chemokines [IFN-γ-inducible protein of 10 kD (IP-10/CXCL10), monokine induced by IFN-γ (Mig/CXCL9), IFN-inducible T cell α chemoattractant (I-TAC/CXCL11)] and those for CCR4 [macrophage-derived chemokine (MDC/CCL22), thymus- and activation-regulated chemokine (TARC/CCL17)] have been shown to play the central roles for T helper-cell recruitment into the tissues. To examine the role of these chemokines in tumor progression of lung cancer, we investigated their expression in human lung cancer tissues to determine the possible relationship between their expression and the prognosis of patients. Methods: Total RNA was prepared from lung cancer tissues of 40 patients (24 adenocarcinoma and 16 squamous cell carcinoma). We measured gene expression levels of chemokines (IP-10, Mig, I-TAC, MDC and TARC) by real-time quantitative RT-PCR. Results: Higher gene expression of MDC in lung cancer was significantly correlated with longer disease-free survival time and lower risk of recurrence after tumor resection. We could not find any significant relationship of IP-10, Mig, I-TAC and TARC gene expression with disease-free survival or lower risk of recurrence after surgery. Conclusions: These results suggest that increased gene expression of MDC in tumor tissues may be a predictive marker for improving the prognosis of lung cancer.
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U2 - 10.1007/s00262-006-0133-y
DO - 10.1007/s00262-006-0133-y
M3 - Article
C2 - 16453150
AN - SCOPUS:33746864838
SN - 0340-7004
VL - 55
SP - 1320
EP - 1329
JO - Cancer Immunology, Immunotherapy
JF - Cancer Immunology, Immunotherapy
IS - 11
ER -
