Expression of mRNAs for c-myc and branched-chain aminotransferases in human gastric cancer cells and tissues

Tetsuya Kato, Hidemi Goto, Yasumasa Niwa, Naoki Omiya, Tetsuo Hayakawa, Taro Murakami, Naoya Nakai, Susan M. Hutson, Yoshiharu Shimomura

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

It has been established that there are two isoforms of mammalian branched-chain aminotransferases, cytosolic (BCATc) and mitochondrial (BCATm). Since the BCATc was reported to be a direct target for c-Myc regulation, we investigated expression of mRNAs for c-myc, BCATc, and BCATm in human gastric cancer cells and tissues., MKN45 and KATOIII were used as human gastric cancer cell lines. Human gastric mucosal tissues with cancer were obtained from 9 patients and those with non-ulcer dyspepsia (NUD) were from 8 patients by endoscopic biopsy. Expression of mRNA was measured by the method of polymerase-chain reaction coupled with reverse transcription. Expression of c-myc mRNA was detected in both cell lines but was greater in MKN45. Expression of BCATc mRNA was detected in KATOIII, but not in MKN45, whereas expression of BCATm mRNA was detected only in MKN45. In the analyses of human gastric tissues, it was found that c-myc mRNA was expressed in all of the tissues examined, but overexpression of the mRNA was detected in the 5 tissues with cancer. On the other hand, expression of BCATc mRNA was detected in 4 tissues with gastric cancer, in which only one tissue had the overexpression of c-myc mRNA. Expression of BCATc mRNA was not detected in all tissues with NUD. BCATm mRNA expression was detected in all tissues with cancer or NUD. These results suggest that there is no correlation between expression of mRNAs for c-myc and BCATc in these tissues and that expression of BCATc in gastric cancer cells and tissues is regulated in a manner independent of c-myc expression.

Original languageEnglish
Pages (from-to)29-36
Number of pages8
JournalJournal of Clinical Biochemistry and Nutrition
Volume29
DOIs
Publication statusPublished - 01-01-2000
Externally publishedYes

Fingerprint

Stomach Neoplasms
Cells
Tissue
Messenger RNA
Dyspepsia
Stomach
branched-chain-amino-acid transaminase
Cell Line
Neoplasms
Biopsy
Polymerase chain reaction
Transcription
Reverse Transcription
Protein Isoforms
Mucous Membrane
Polymerase Chain Reaction

All Science Journal Classification (ASJC) codes

  • Medicine (miscellaneous)
  • Nutrition and Dietetics
  • Clinical Biochemistry

Cite this

Kato, Tetsuya ; Goto, Hidemi ; Niwa, Yasumasa ; Omiya, Naoki ; Hayakawa, Tetsuo ; Murakami, Taro ; Nakai, Naoya ; Hutson, Susan M. ; Shimomura, Yoshiharu. / Expression of mRNAs for c-myc and branched-chain aminotransferases in human gastric cancer cells and tissues. In: Journal of Clinical Biochemistry and Nutrition. 2000 ; Vol. 29. pp. 29-36.
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abstract = "It has been established that there are two isoforms of mammalian branched-chain aminotransferases, cytosolic (BCATc) and mitochondrial (BCATm). Since the BCATc was reported to be a direct target for c-Myc regulation, we investigated expression of mRNAs for c-myc, BCATc, and BCATm in human gastric cancer cells and tissues., MKN45 and KATOIII were used as human gastric cancer cell lines. Human gastric mucosal tissues with cancer were obtained from 9 patients and those with non-ulcer dyspepsia (NUD) were from 8 patients by endoscopic biopsy. Expression of mRNA was measured by the method of polymerase-chain reaction coupled with reverse transcription. Expression of c-myc mRNA was detected in both cell lines but was greater in MKN45. Expression of BCATc mRNA was detected in KATOIII, but not in MKN45, whereas expression of BCATm mRNA was detected only in MKN45. In the analyses of human gastric tissues, it was found that c-myc mRNA was expressed in all of the tissues examined, but overexpression of the mRNA was detected in the 5 tissues with cancer. On the other hand, expression of BCATc mRNA was detected in 4 tissues with gastric cancer, in which only one tissue had the overexpression of c-myc mRNA. Expression of BCATc mRNA was not detected in all tissues with NUD. BCATm mRNA expression was detected in all tissues with cancer or NUD. These results suggest that there is no correlation between expression of mRNAs for c-myc and BCATc in these tissues and that expression of BCATc in gastric cancer cells and tissues is regulated in a manner independent of c-myc expression.",
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Expression of mRNAs for c-myc and branched-chain aminotransferases in human gastric cancer cells and tissues. / Kato, Tetsuya; Goto, Hidemi; Niwa, Yasumasa; Omiya, Naoki; Hayakawa, Tetsuo; Murakami, Taro; Nakai, Naoya; Hutson, Susan M.; Shimomura, Yoshiharu.

In: Journal of Clinical Biochemistry and Nutrition, Vol. 29, 01.01.2000, p. 29-36.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Expression of mRNAs for c-myc and branched-chain aminotransferases in human gastric cancer cells and tissues

AU - Kato, Tetsuya

AU - Goto, Hidemi

AU - Niwa, Yasumasa

AU - Omiya, Naoki

AU - Hayakawa, Tetsuo

AU - Murakami, Taro

AU - Nakai, Naoya

AU - Hutson, Susan M.

AU - Shimomura, Yoshiharu

PY - 2000/1/1

Y1 - 2000/1/1

N2 - It has been established that there are two isoforms of mammalian branched-chain aminotransferases, cytosolic (BCATc) and mitochondrial (BCATm). Since the BCATc was reported to be a direct target for c-Myc regulation, we investigated expression of mRNAs for c-myc, BCATc, and BCATm in human gastric cancer cells and tissues., MKN45 and KATOIII were used as human gastric cancer cell lines. Human gastric mucosal tissues with cancer were obtained from 9 patients and those with non-ulcer dyspepsia (NUD) were from 8 patients by endoscopic biopsy. Expression of mRNA was measured by the method of polymerase-chain reaction coupled with reverse transcription. Expression of c-myc mRNA was detected in both cell lines but was greater in MKN45. Expression of BCATc mRNA was detected in KATOIII, but not in MKN45, whereas expression of BCATm mRNA was detected only in MKN45. In the analyses of human gastric tissues, it was found that c-myc mRNA was expressed in all of the tissues examined, but overexpression of the mRNA was detected in the 5 tissues with cancer. On the other hand, expression of BCATc mRNA was detected in 4 tissues with gastric cancer, in which only one tissue had the overexpression of c-myc mRNA. Expression of BCATc mRNA was not detected in all tissues with NUD. BCATm mRNA expression was detected in all tissues with cancer or NUD. These results suggest that there is no correlation between expression of mRNAs for c-myc and BCATc in these tissues and that expression of BCATc in gastric cancer cells and tissues is regulated in a manner independent of c-myc expression.

AB - It has been established that there are two isoforms of mammalian branched-chain aminotransferases, cytosolic (BCATc) and mitochondrial (BCATm). Since the BCATc was reported to be a direct target for c-Myc regulation, we investigated expression of mRNAs for c-myc, BCATc, and BCATm in human gastric cancer cells and tissues., MKN45 and KATOIII were used as human gastric cancer cell lines. Human gastric mucosal tissues with cancer were obtained from 9 patients and those with non-ulcer dyspepsia (NUD) were from 8 patients by endoscopic biopsy. Expression of mRNA was measured by the method of polymerase-chain reaction coupled with reverse transcription. Expression of c-myc mRNA was detected in both cell lines but was greater in MKN45. Expression of BCATc mRNA was detected in KATOIII, but not in MKN45, whereas expression of BCATm mRNA was detected only in MKN45. In the analyses of human gastric tissues, it was found that c-myc mRNA was expressed in all of the tissues examined, but overexpression of the mRNA was detected in the 5 tissues with cancer. On the other hand, expression of BCATc mRNA was detected in 4 tissues with gastric cancer, in which only one tissue had the overexpression of c-myc mRNA. Expression of BCATc mRNA was not detected in all tissues with NUD. BCATm mRNA expression was detected in all tissues with cancer or NUD. These results suggest that there is no correlation between expression of mRNAs for c-myc and BCATc in these tissues and that expression of BCATc in gastric cancer cells and tissues is regulated in a manner independent of c-myc expression.

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