TY - JOUR
T1 - Expression of N-acetylglucosaminyltransferase V in endometrial cancer correlates with poor prognosis
AU - Yamamoto, E.
AU - Ino, K.
AU - Miyoshi, E.
AU - Shibata, K.
AU - Takahashi, N.
AU - Kajiyama, H.
AU - Nawa, A.
AU - Nomura, S.
AU - Nagasaka, T.
AU - Kikkawa, F.
N1 - Funding Information:
This work was supported by Grants-in-aid no.18799005 (to EY) from the Japanese Ministry of Education, Culture, Sports, Science, and Technology.
PY - 2007/12/3
Y1 - 2007/12/3
N2 - N-acetylglucosaminyltransferase V (GnT-V) is an enzyme that catalyses β1-6 branching of N-acetylglucosamine on asparagine-linked oligosaccharides of cell proteins. The present study aimed to investigate GnT-V expression and its prognostic significance in endometrial cancer. N- acetylglucosaminyltransferase V expression was studied by immunohistochemistry in 74 surgically resected endometrial cancers, and the staining intensity was evaluated. High GnT-V expression in tumour cells was found in 43 (58.1%) of the 74 cases, and was positively correlated with advanced patient age, histological grade, and lymph vascular space involvement. Patients with high GnT-V expression had significantly impaired overall survival and progression-free survival (PFS) (P=0.0041 and P=0.0023, respectively) compared to patients with low expression of GnT-V. On multivariate analysis, GnT-V expression was an independent prognostic factor for PFS (P=0.0364). β1-6 branching of asparagine-linked oligosaccharides was also detected in GnT-V-positive endometrial cancer cells by leukoagglutinating phytohaemagglutinin (L4-PHA) staining, and the molecular size of the major glycoproteins recognised by L4-PHA was approximately 60-200 kDa by lectin blot analysis. These results suggested that high GnT-V expression was correlated with an unfavourable clinical outcome, and that GnT-V is involved in the malignant potential of endometrial cancer by increasing the synthesis of β1-6 branching of asparagine-linked oligosaccharides.
AB - N-acetylglucosaminyltransferase V (GnT-V) is an enzyme that catalyses β1-6 branching of N-acetylglucosamine on asparagine-linked oligosaccharides of cell proteins. The present study aimed to investigate GnT-V expression and its prognostic significance in endometrial cancer. N- acetylglucosaminyltransferase V expression was studied by immunohistochemistry in 74 surgically resected endometrial cancers, and the staining intensity was evaluated. High GnT-V expression in tumour cells was found in 43 (58.1%) of the 74 cases, and was positively correlated with advanced patient age, histological grade, and lymph vascular space involvement. Patients with high GnT-V expression had significantly impaired overall survival and progression-free survival (PFS) (P=0.0041 and P=0.0023, respectively) compared to patients with low expression of GnT-V. On multivariate analysis, GnT-V expression was an independent prognostic factor for PFS (P=0.0364). β1-6 branching of asparagine-linked oligosaccharides was also detected in GnT-V-positive endometrial cancer cells by leukoagglutinating phytohaemagglutinin (L4-PHA) staining, and the molecular size of the major glycoproteins recognised by L4-PHA was approximately 60-200 kDa by lectin blot analysis. These results suggested that high GnT-V expression was correlated with an unfavourable clinical outcome, and that GnT-V is involved in the malignant potential of endometrial cancer by increasing the synthesis of β1-6 branching of asparagine-linked oligosaccharides.
KW - Endometrial cancer
KW - N-acetylglucosaminyltransferase V
KW - Prognostic factor
KW - Progression-free survival (PFS)
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UR - http://www.scopus.com/inward/citedby.url?scp=36448985316&partnerID=8YFLogxK
U2 - 10.1038/sj.bjc.6604044
DO - 10.1038/sj.bjc.6604044
M3 - Article
C2 - 17971775
AN - SCOPUS:36448985316
SN - 0007-0920
VL - 97
SP - 1538
EP - 1544
JO - British Journal of Cancer
JF - British Journal of Cancer
IS - 11
ER -
