TY - JOUR
T1 - Expression of protein kinase c isozyme in epidermal langerhans cells of the mouse
AU - Koyama, Yoh ichi
AU - Hachiya, Takahisa
AU - Hagiwara, Masatoshi
AU - Kobayashi, Miya
AU - Ohashi, Koji
AU - Hoshino, Takeshi
AU - Hidaka, Hiroyoshi
AU - Marunouchi, Tohru
PY - 1990/5
Y1 - 1990/5
N2 - Protein kinase C (PKC) is encoded by a complex of a gene family, and its multiple isozymes are expressed in various mammalian tissues. We examined whether PKC is expressed in epidermal Langerhans cells (LC) of the mouse by using monoclonal antibodies specific to PKC I, PKC II, and PKC III isozymes (respective products of PKC genes γ, β, and α). Immunohistochemical and immunoblotting studies revealed that LC of adult C57BL/6 mice express PKC II, while PKC I and PKC III were not detected. In keratinocytes, none of the isozymes were detected. These results suggest that PKC II is a novel marker of LC and that it possibly plays a regulatory role in epidermal LC of the mouse in vivo.
AB - Protein kinase C (PKC) is encoded by a complex of a gene family, and its multiple isozymes are expressed in various mammalian tissues. We examined whether PKC is expressed in epidermal Langerhans cells (LC) of the mouse by using monoclonal antibodies specific to PKC I, PKC II, and PKC III isozymes (respective products of PKC genes γ, β, and α). Immunohistochemical and immunoblotting studies revealed that LC of adult C57BL/6 mice express PKC II, while PKC I and PKC III were not detected. In keratinocytes, none of the isozymes were detected. These results suggest that PKC II is a novel marker of LC and that it possibly plays a regulatory role in epidermal LC of the mouse in vivo.
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U2 - 10.1111/1523-1747.ep12876255
DO - 10.1111/1523-1747.ep12876255
M3 - Article
C2 - 2324524
AN - SCOPUS:0025267207
SN - 0022-202X
VL - 94
SP - 677
EP - 680
JO - Journal of Investigative Dermatology
JF - Journal of Investigative Dermatology
IS - 5
ER -