Expression of protein kinase c isozyme in epidermal langerhans cells of the mouse

Yoh ichi Koyama, Takahisa Hachiya, Masatoshi Hagiwara, Miya Kobayashi, Koji Ohashi, Takeshi Hoshino, Hiroyoshi Hidaka, Tohru Marunouchi

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Protein kinase C (PKC) is encoded by a complex of a gene family, and its multiple isozymes are expressed in various mammalian tissues. We examined whether PKC is expressed in epidermal Langerhans cells (LC) of the mouse by using monoclonal antibodies specific to PKC I, PKC II, and PKC III isozymes (respective products of PKC genes γ, β, and α). Immunohistochemical and immunoblotting studies revealed that LC of adult C57BL/6 mice express PKC II, while PKC I and PKC III were not detected. In keratinocytes, none of the isozymes were detected. These results suggest that PKC II is a novel marker of LC and that it possibly plays a regulatory role in epidermal LC of the mouse in vivo.

Original languageEnglish
Pages (from-to)677-680
Number of pages4
JournalJournal of Investigative Dermatology
Volume94
Issue number5
DOIs
Publication statusPublished - 01-01-1990

Fingerprint

Langerhans Cells
Protein Kinases
Protein Kinase C
Isoenzymes
Cells
Genes
Inbred C57BL Mouse
Keratinocytes
Immunoblotting
Monoclonal Antibodies
Tissue

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Dermatology
  • Cell Biology

Cite this

Koyama, Y. I., Hachiya, T., Hagiwara, M., Kobayashi, M., Ohashi, K., Hoshino, T., ... Marunouchi, T. (1990). Expression of protein kinase c isozyme in epidermal langerhans cells of the mouse. Journal of Investigative Dermatology, 94(5), 677-680. https://doi.org/10.1111/1523-1747.ep12876255
Koyama, Yoh ichi ; Hachiya, Takahisa ; Hagiwara, Masatoshi ; Kobayashi, Miya ; Ohashi, Koji ; Hoshino, Takeshi ; Hidaka, Hiroyoshi ; Marunouchi, Tohru. / Expression of protein kinase c isozyme in epidermal langerhans cells of the mouse. In: Journal of Investigative Dermatology. 1990 ; Vol. 94, No. 5. pp. 677-680.
@article{07cdbcd77be14108ad27aa34e3aeb9d7,
title = "Expression of protein kinase c isozyme in epidermal langerhans cells of the mouse",
abstract = "Protein kinase C (PKC) is encoded by a complex of a gene family, and its multiple isozymes are expressed in various mammalian tissues. We examined whether PKC is expressed in epidermal Langerhans cells (LC) of the mouse by using monoclonal antibodies specific to PKC I, PKC II, and PKC III isozymes (respective products of PKC genes γ, β, and α). Immunohistochemical and immunoblotting studies revealed that LC of adult C57BL/6 mice express PKC II, while PKC I and PKC III were not detected. In keratinocytes, none of the isozymes were detected. These results suggest that PKC II is a novel marker of LC and that it possibly plays a regulatory role in epidermal LC of the mouse in vivo.",
author = "Koyama, {Yoh ichi} and Takahisa Hachiya and Masatoshi Hagiwara and Miya Kobayashi and Koji Ohashi and Takeshi Hoshino and Hiroyoshi Hidaka and Tohru Marunouchi",
year = "1990",
month = "1",
day = "1",
doi = "10.1111/1523-1747.ep12876255",
language = "English",
volume = "94",
pages = "677--680",
journal = "Journal of Investigative Dermatology",
issn = "0022-202X",
publisher = "Nature Publishing Group",
number = "5",

}

Koyama, YI, Hachiya, T, Hagiwara, M, Kobayashi, M, Ohashi, K, Hoshino, T, Hidaka, H & Marunouchi, T 1990, 'Expression of protein kinase c isozyme in epidermal langerhans cells of the mouse', Journal of Investigative Dermatology, vol. 94, no. 5, pp. 677-680. https://doi.org/10.1111/1523-1747.ep12876255

Expression of protein kinase c isozyme in epidermal langerhans cells of the mouse. / Koyama, Yoh ichi; Hachiya, Takahisa; Hagiwara, Masatoshi; Kobayashi, Miya; Ohashi, Koji; Hoshino, Takeshi; Hidaka, Hiroyoshi; Marunouchi, Tohru.

In: Journal of Investigative Dermatology, Vol. 94, No. 5, 01.01.1990, p. 677-680.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Expression of protein kinase c isozyme in epidermal langerhans cells of the mouse

AU - Koyama, Yoh ichi

AU - Hachiya, Takahisa

AU - Hagiwara, Masatoshi

AU - Kobayashi, Miya

AU - Ohashi, Koji

AU - Hoshino, Takeshi

AU - Hidaka, Hiroyoshi

AU - Marunouchi, Tohru

PY - 1990/1/1

Y1 - 1990/1/1

N2 - Protein kinase C (PKC) is encoded by a complex of a gene family, and its multiple isozymes are expressed in various mammalian tissues. We examined whether PKC is expressed in epidermal Langerhans cells (LC) of the mouse by using monoclonal antibodies specific to PKC I, PKC II, and PKC III isozymes (respective products of PKC genes γ, β, and α). Immunohistochemical and immunoblotting studies revealed that LC of adult C57BL/6 mice express PKC II, while PKC I and PKC III were not detected. In keratinocytes, none of the isozymes were detected. These results suggest that PKC II is a novel marker of LC and that it possibly plays a regulatory role in epidermal LC of the mouse in vivo.

AB - Protein kinase C (PKC) is encoded by a complex of a gene family, and its multiple isozymes are expressed in various mammalian tissues. We examined whether PKC is expressed in epidermal Langerhans cells (LC) of the mouse by using monoclonal antibodies specific to PKC I, PKC II, and PKC III isozymes (respective products of PKC genes γ, β, and α). Immunohistochemical and immunoblotting studies revealed that LC of adult C57BL/6 mice express PKC II, while PKC I and PKC III were not detected. In keratinocytes, none of the isozymes were detected. These results suggest that PKC II is a novel marker of LC and that it possibly plays a regulatory role in epidermal LC of the mouse in vivo.

UR - http://www.scopus.com/inward/record.url?scp=0025267207&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0025267207&partnerID=8YFLogxK

U2 - 10.1111/1523-1747.ep12876255

DO - 10.1111/1523-1747.ep12876255

M3 - Article

C2 - 2324524

AN - SCOPUS:0025267207

VL - 94

SP - 677

EP - 680

JO - Journal of Investigative Dermatology

JF - Journal of Investigative Dermatology

SN - 0022-202X

IS - 5

ER -