Expression of ret proto-oncogene products in the hypoganglionic segment of the small intestine of congenital aganglionosis rats

Yoshio Watanabe, Takahiro Ito, Toru Harada, Masahide Takahashi, Shigeru Kobayashi, Tuyoshi Ozaki, Yuji Nimura

Research output: Contribution to journalArticlepeer-review

7 Citations (Scopus)

Abstract

The ret proto-oncogene (proto-ret) encodes a receptor tyrosine kinase that contains a cadherin-related sequence in the extracellular domain, which plays an important role in control of normal cell growth and differentiation. Recent studies have shown that familial hirschsprung's disease is closely linked to the proto-ret locus. Immunohistological studies on congenital aganglionosis rats were made using polyclonal antibody generated for the carboxy-terminal sequence of the proto-ret products. Immunoreactivity was investigated in the small and large intestines of mutant and control rats. It was increased in the ganglia of the hypoganglionic segment of the small intestine and was minimal in the normoganglionic small intestinal segment of congenital aganglionosis in rats. The reactivity was also strong in the ganglia of the large intestine of normal rats. Because the proto-ret is preferentially expressed in the neuronal tissue and plays a critical role in differentiation of apparently multipotential neural crest cells to enteric neurons, the results suggest that abnormal development of local vagal neural crest cells, which were influenced by spatially regulating factors, results in the hypoganglionic segment.

Original languageEnglish
Pages (from-to)641-645
Number of pages5
JournalJournal of Pediatric Surgery
Volume30
Issue number5
DOIs
Publication statusPublished - 05-1995
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Surgery
  • Pediatrics, Perinatology, and Child Health

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