Expression of the tyrosinase-encoding gene in a colorless melanophore mutant of the medaka fish, Oryzias latipes

Hidehito Inagaki, Bessho Yoshitaka, Koga Akihiko, Hori Hiroshi

Research output: Contribution to journalArticle

48 Citations (Scopus)

Abstract

In the medaka fish Oryzias latipes many mutants for body colors have been isolated. Among them, a colorless melanophore mutant b, carrying b alleles homozygously, has pigmented black eyes but orange-colored skin with amelanotic melanophores, suggesting the presence of a tissue-specific mechanism of melanin formation. To cast light on the molecular basis of the mechanism, we have cloned cDNAs for tyrosinase (Tyr), a key enzyme in melanin biosynthesis, from the wild-type (wt) fish. DNA sequence analysis revealed that all clones encode a protein of 540 amino acids, having five potential glycosylation sites and two copper-binding sites that are characteristic features of Tyr. Genomic DNA blot analysis disclosed that the Tyr gene is present as a single copy in the fish genome. Using a cDNA clone as a probe, RNA blot analysis was carried out. In the wt, the 2.2-kb Tyr mRNA was expressed in eyes and skin but not in liver, corresponding to tissue-specific melanin formation. In the b mutant, contrary to expectation, the mRNA was detected not only in eyes but also in amelanotic skin. Therefore, pigmentation of the skin controlled by b is not directly related to expression of the Tyr gene.

Original languageEnglish
Pages (from-to)319-324
Number of pages6
JournalGene
Volume150
Issue number2
DOIs
Publication statusPublished - 01-01-1994

Fingerprint

Melanophores
Oryzias
Monophenol Monooxygenase
Fishes
Melanins
Genes
Skin
Complementary DNA
Clone Cells
Skin Pigmentation
RNA Probes
Messenger RNA
DNA Sequence Analysis
Glycosylation
Copper
Color
Alleles
Binding Sites
Genome
Gene Expression

All Science Journal Classification (ASJC) codes

  • Genetics

Cite this

Inagaki, Hidehito ; Yoshitaka, Bessho ; Akihiko, Koga ; Hiroshi, Hori. / Expression of the tyrosinase-encoding gene in a colorless melanophore mutant of the medaka fish, Oryzias latipes. In: Gene. 1994 ; Vol. 150, No. 2. pp. 319-324.
@article{8d6d6f41850144f783aedfd342256509,
title = "Expression of the tyrosinase-encoding gene in a colorless melanophore mutant of the medaka fish, Oryzias latipes",
abstract = "In the medaka fish Oryzias latipes many mutants for body colors have been isolated. Among them, a colorless melanophore mutant b, carrying b alleles homozygously, has pigmented black eyes but orange-colored skin with amelanotic melanophores, suggesting the presence of a tissue-specific mechanism of melanin formation. To cast light on the molecular basis of the mechanism, we have cloned cDNAs for tyrosinase (Tyr), a key enzyme in melanin biosynthesis, from the wild-type (wt) fish. DNA sequence analysis revealed that all clones encode a protein of 540 amino acids, having five potential glycosylation sites and two copper-binding sites that are characteristic features of Tyr. Genomic DNA blot analysis disclosed that the Tyr gene is present as a single copy in the fish genome. Using a cDNA clone as a probe, RNA blot analysis was carried out. In the wt, the 2.2-kb Tyr mRNA was expressed in eyes and skin but not in liver, corresponding to tissue-specific melanin formation. In the b mutant, contrary to expectation, the mRNA was detected not only in eyes but also in amelanotic skin. Therefore, pigmentation of the skin controlled by b is not directly related to expression of the Tyr gene.",
author = "Hidehito Inagaki and Bessho Yoshitaka and Koga Akihiko and Hori Hiroshi",
year = "1994",
month = "1",
day = "1",
doi = "10.1016/0378-1119(94)90445-6",
language = "English",
volume = "150",
pages = "319--324",
journal = "Gene",
issn = "0378-1119",
publisher = "Elsevier",
number = "2",

}

Expression of the tyrosinase-encoding gene in a colorless melanophore mutant of the medaka fish, Oryzias latipes. / Inagaki, Hidehito; Yoshitaka, Bessho; Akihiko, Koga; Hiroshi, Hori.

In: Gene, Vol. 150, No. 2, 01.01.1994, p. 319-324.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Expression of the tyrosinase-encoding gene in a colorless melanophore mutant of the medaka fish, Oryzias latipes

AU - Inagaki, Hidehito

AU - Yoshitaka, Bessho

AU - Akihiko, Koga

AU - Hiroshi, Hori

PY - 1994/1/1

Y1 - 1994/1/1

N2 - In the medaka fish Oryzias latipes many mutants for body colors have been isolated. Among them, a colorless melanophore mutant b, carrying b alleles homozygously, has pigmented black eyes but orange-colored skin with amelanotic melanophores, suggesting the presence of a tissue-specific mechanism of melanin formation. To cast light on the molecular basis of the mechanism, we have cloned cDNAs for tyrosinase (Tyr), a key enzyme in melanin biosynthesis, from the wild-type (wt) fish. DNA sequence analysis revealed that all clones encode a protein of 540 amino acids, having five potential glycosylation sites and two copper-binding sites that are characteristic features of Tyr. Genomic DNA blot analysis disclosed that the Tyr gene is present as a single copy in the fish genome. Using a cDNA clone as a probe, RNA blot analysis was carried out. In the wt, the 2.2-kb Tyr mRNA was expressed in eyes and skin but not in liver, corresponding to tissue-specific melanin formation. In the b mutant, contrary to expectation, the mRNA was detected not only in eyes but also in amelanotic skin. Therefore, pigmentation of the skin controlled by b is not directly related to expression of the Tyr gene.

AB - In the medaka fish Oryzias latipes many mutants for body colors have been isolated. Among them, a colorless melanophore mutant b, carrying b alleles homozygously, has pigmented black eyes but orange-colored skin with amelanotic melanophores, suggesting the presence of a tissue-specific mechanism of melanin formation. To cast light on the molecular basis of the mechanism, we have cloned cDNAs for tyrosinase (Tyr), a key enzyme in melanin biosynthesis, from the wild-type (wt) fish. DNA sequence analysis revealed that all clones encode a protein of 540 amino acids, having five potential glycosylation sites and two copper-binding sites that are characteristic features of Tyr. Genomic DNA blot analysis disclosed that the Tyr gene is present as a single copy in the fish genome. Using a cDNA clone as a probe, RNA blot analysis was carried out. In the wt, the 2.2-kb Tyr mRNA was expressed in eyes and skin but not in liver, corresponding to tissue-specific melanin formation. In the b mutant, contrary to expectation, the mRNA was detected not only in eyes but also in amelanotic skin. Therefore, pigmentation of the skin controlled by b is not directly related to expression of the Tyr gene.

UR - http://www.scopus.com/inward/record.url?scp=0028559432&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0028559432&partnerID=8YFLogxK

U2 - 10.1016/0378-1119(94)90445-6

DO - 10.1016/0378-1119(94)90445-6

M3 - Article

C2 - 7821799

AN - SCOPUS:0028559432

VL - 150

SP - 319

EP - 324

JO - Gene

JF - Gene

SN - 0378-1119

IS - 2

ER -